A Significant Upregulation of miR-886-5p in High Grade and Invasive Bladder Tumors
Urology Journal,
Vol. 12 No. 3 (2015),
1 July 2015
,
Page 2160-2164
https://doi.org/10.22037/uj.v12i3.2222
Abstract
Purpose: To investigate the expression alteration of miR-886-5p in bladder tumors and evaluating its expression level as a potential biomarker in this type of cancer.
Materials and Methods: Formalin-fixed paraffin-embedded (FFPE) samples of bladder tumors belonging to 70 patients whom had been referred to the Shahid Labbafi-Nejad medical center were obtained from the archival collection of pathology department. After RNA extraction and cDNA synthesis, expression levels of miR-886-5p were quantified by a real-time reverse transcription polymerase chain reaction (RT-PCR) approach.
Results: Our data revealed a significant upregulation (~3 times) of miR-886-5p in high grade bladder tumors, compared to the low grade ones (P < .05). Moreover, its expression level could significantly discriminate noninvasive (Ta, T1) from invasive (T2-T4) tumor stages.
Conclusion: Our data suggests a potential role for miR-886-5p in progression of bladder cancer.
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