Comparison the antimicrobial effect of methanolic total extracts and petroleum ether fractions of flowering aerial parts of Glaucium vitellinum Boiss. & Buhse and Gaillonia aucheri Jaub. & Spach
Novelty in Biomedicine,
Vol. 5 No. 1 (2017),
19 January 2017
,
Page 24-29
https://doi.org/10.22037/nbm.v5i1.9836
Abstract
Background: With increasing use of antibiotics, the number and type of microbial resistance to antibiotics have been raised and at result the need for synthesis of new antimicrobials or acquire new sources of antimicrobial is indisputable. In this study the antimicrobial effects of the flowering aerial parts of Glaucium vitellinum and Gaillonia aucheri were investigated.Materials and Methods: The antimicrobial effect of mentioned extracts against Escherichia coli PTCC 1399, Pseudomonas aeruginosa PTCC 1430, Salmonella typhimurium PTCC 1639, Staphylococcus aureus PTCC 1431 and Candida albicans PTCC 5027 were evaluated by disk diffusion and the microdilution method based on CLSI protocol 2012.
Results: No any zone of inhibition were detected by disk diffusion method against tested microbes for Glaucium vitellinum. After deletion of agar interference, the minimal inhibitory concentration (MIC) was determined by broth microdilution method for two plants. None of tested extracts were effective against Gram negative tested bacteria except alkaloid fraction of G. vitellinum which was effective against S. typhi. In contrast, the extracts and fractions of both plans were effective against tested gram positive bacteria especially S. aureus.
Conclusion: The best result of MIC was detected for alkaloid fraction of G. vitellinum (0.09 mg/ml) Vs. G. aucheri (125 mg/ml) against S.aureus.
- Gaillonia aucheri
- Glaucium vitellinum
- antibacterial agents
- antifungal agents
- MIC
How to Cite
References
Halakou M. Evaluation of antifungal activity of total extract and alkaloids of Glacium. Pharmacy Thesis, Faculty of Pharmacy, Islamic Azad University:7
Lalezari, letal. Major alkaloids of Glacium flvum crantz population Ghom. J. Pharm Sci. 1976, 56 (6): 923-4
Tyler, Brady & Robbers. Pharmacognosy. 9th Edition. Philadelphia; Lea & Fibiger.1988: 473.
Amin Gh. Traditional medicinal plants of Iran. Ministry of Health Research Deputy Publication.2005(1):23-24
Izaddoust M. Chemical Plant Tehran University Publication. 1984: 18-19
Momeni T. Plant extracts. Shahid Farhad Reza Publications. 2000. 10-20
Javasinghe VL, Jayasooriya CP. Antimicrobial activity of some Srilankan Rubiaceae and Meliaceae. Fititerapia. 2002, 73(5):424-427.
Ninoj, Narvaez DM, Mosquera OM. Antibacterial, antifungal and cytotoxic activities of eight Asteraceae and two Rubiaceae plants from Colombian Biodiversity. Brazilian J. microbial. 2006(37): 566-570.
Parthasarathy S, Azizi JB, Ramanathan S. Evaluation of antioxidant and antibacterial activities of aqueus, methanolic and alkaloid extracts from Mitragyna speciosa) Rubiaceae Family) leaves. Molecules. 2009, 14 (10): 3964-3974.
Comini LR. Montoya SCN, Paez PL. Antibacterial activity of anthraquinone derivatives from Hetrophyllaea pustulata (Rubiaceae). J. phytochem. phytobial. 2010, 102(2):108-114.
Rubiacea family. Available from: http://plantbio.ir/ftopic-107-next.html. Accessed August 2013.
Draelos Z.D. Cosmetics in dermatology. USA: Noyes publications; 1995: 214-220, 252.
11- Meyers Robert A. Encyclopedia of Physical Science and Technology- Alkaloids. 3rd edition. Academic Press, 2001: 0-12, 411-413.
Mallik [etal]. Phytochemical and Preliminary Toxicity study of Sesbania grandiflora (LiNN). Flowers. IJBAR. 2011, 02(11): 444-448.
Prashant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur. Phytochemical sceening & Extraction. International pharmaceutica sciencia.2011,(1) Available online http://www.ipharmsciencia.com.
Patrik R, Murray kens. Rosenthal George S. Kobayash. Medical Microbiology. 5th edi. Mosby. 2005: 202, 266, 288, 277.
Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial susceptibility testing. Twenty-second informational supplement. Document M100-S22 Wayne PC: 2012
Baren. Ellen Jo, [et al]. Diagnostic microbiology. 8th edi. C.V: Mosby company. 1990: 181-184.
Hugo W.B, Russell A.D. pharmaceutical microbiology. 6th ed. Black well science.1998: 242-244.
Comparison of disk diffusion, agar dilution, and broth microdilution for antimicrobial susceptibility testing of five chitosans.(2011). A Thesis submitted to the department of food and science, Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College. By Lin Jiang.
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