Enrichment of Acanthamoeba Culture Medium Using TYIS 33 Medium: a Step toward a Successful Axenic Cultivation
Novelty in Biomedicine,
Vol. 3 No. 2 (2015),
12 Farvardin 2015
,
Page 69-72
https://doi.org/10.22037/nbm.v3i2.7808
Abstract
Background: Acanthamoeba-related disease have a poor prognosis according to many previous studies. Thus researches regarding biochemical and molecular aspects of this organism are a high priority. To this end achieving high amount of amoebae in culture is the first step for such studies. The main aim of the present research was to address the usage of TYI-S-33 (Tripticase, Yeast extract, iron-serum) medium as an enrichment component for achieving high and fast growth of trophozoites in agar culture medium within 24 hours.Materials and Methods: Overall, 10 Acanthamoeba strains were cultured, cloned and genotyped and the cultures were then enriched with addition of TYI-S-33 medium. Amoebae growth was then monitored daily. Ten plates also were used without addition of TYI-S-33 medium.
Results: The result of the present research revealed that addition of TYI-S-33 medium is a promising approach for obtaining 100% trophozoites within 24 hours of culture.
Conclusion: To the best of our knowledge this is the first report of successful achieving high amount of trophozoites within short time that able researchers to arrange molecular and biochemical assays
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References
Rezaeian M, Niyyati M. Pathogenic Free Living Amebas In Human” 1st ed. Tehran: TUMS Publication. 2010.
Visvesvara GS, Moura H, Schuster FL. Pathogenic and opportunistic free living amoebae: Acanthamoeba spp, Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol and Med Microbiol. 2007;50(1):1-26.
Khan NA. Acanthamoeba, biology and pathogenesis”. 1st ed.Great Britine: Caister Academic Press. 2009.
Marciano-Cabral FM and Cabral G. Acanthamoeba spp. as Agents of Disease in Humans. Clin Microbiol Rev. 2003;16(2):273-307.
Khan NA. Pathogenesis of Acanthamoeba infections. Microb pathog. 2003;34:277-85.
Khan NA. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol Rev. 2006;30:564–95.
Khan NA, Jarroll EL, Paget TA. Molecular and physiological differentiation between pathogenic and nonpathogenic Acanthamoeba. Curr Microbiol. 2002;45:197–202.
Khan NA, Jarroll EL, Panjwani N, Cao Z, Pager TA. Proteases as markers for differentiation of pathogenic and non pathogenic species of Acanthamoeba. J Clin Microbiol. 2000;38(8):2858-61.
Schuster FL, Visvesvara GS. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Inter J Parasitol. 2004;34(9):1001-27.
Clark CG and Diamond LS. Methods for cultivation of luminal parasitic protists of clinical importance. Clin Microbiol Rev. 2002;15(3):328-41.
Schuster FL & Visvesvara GS. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Int J Parasitol. 2004;34:1001–27
Lorenzo-Morales J, Monteverde-Miranda CA, Jimenez C, Tejedor ML, Valladares B, Ortega-Rivas. Evaluation of Acanthamoeba isolates from environmental sources in Tenerife, Canary Islands, Spain. Ann Agric Environ Med. 2005;12(2):233-6.
Siddiqui R, Khan NA. Balamuthia amoebic encephalitis: An emerging disease with fatal consequences. Microb Pathog. 2008;44:89–97
Niyyati M, Lorenzo-Morales J, Rezaie S, Rahimi F, Mohebali M, Maghsood AH, Motevalli-Haghi A, Martín-Navarro CM, Farnia S, Valladares B, Rezaeian M. Genotyping of Acanthamoeba isolates from clinical and environmental specimens in Iran. Exp Parasitol. 2009;121(3):242-5.
Niyyati M, Lorenzo-Morales J, Rezaie S, Rahimi F, Martín-Navarro CM, Mohebali M, Maghsood AH, Farnia S, Valladares B, Rezaeian M. First report of a mixed infection due to Acanthamoeba genotype T3 and Vahlkampfia in a cosmetic soft contact lens wearer in Iran. Exp Parasitol. 2010;126(1):89-90.
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