Cytoplasmic and membranous CD24 marker expression has indirect correlation with cAMP/cGMP ratio
Novelty in Biomedicine,
Vol. 1 No. 3 (2013),
15 February 2014
,
Page 73-77
https://doi.org/10.22037/nbm.v1i3.5429
Abstract
Background- CD24 is a cell adhesion molecule that has been implicated in metastatic tumor progression cells. Our aim was clarify correlation between CD24 expression and cAMP:cGMP ratio in murine colorectal cancer cell line (CT26) after using cholera toxin.
Materials and Methods- The CT26 cells were cultured in microtubes for assaying cAMP and cGMP; also the cells were cultured in flasks for assaying cytoplasmic and membranous CD24 expression. The Real-Time PCR was done for cDNA that was synthesized from CT26 cells’ mRNA. Also, expression CD24 marker of cells was determined by Anti-CD24 antibody and Goat Anti-Rabbit IgG-FITC (flow cytometry).
Results- The cholera toxin grew cAMP:cGMP ratio and it influenced cytoplasmic and membrane CD24 expression.
Conclusion- There are indirect correlation between cAMP:cGMP ratio and CD24 expression.
- CT26 cell line
- colorectal cancer
- cAMP
- cGMP
- CD24
How to Cite
References
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