Investigating the effects of outer membrane vesicles derived from Campylobacter jejuni on the survival of HT-29 epithelial cells in vitro
Novelty in Biomedicine,
Vol. 13 No. 2 (2025),
19 April 2025
,
Page 93-99
https://doi.org/10.22037/nbm.v13i2.46890
Abstract
Background: Campylobacter jejuni is an important pathogenic bacterium that is associated with diarrhea and gastroenteritis in several animal species and humans. The secretion of virulence factors is a crucial strategy that enteric bacterial pathogens use to interact with host cells, promote their survival, and damage the host. Many bacterial pathogens utilize outer membrane vesicles (OMVs) to deliver virulence factors into host cells. C. jejuni can produce nanosized OMV cargos, which have been proposed to have a key role in disease progression, pathogenesis, and immune system modulation. This study aimed to assess the effect of OMVs derived from C. jejuni on the survival of HT-29 intestinal cells in vitro.
Materials and Methods: In this work, C. jejuni clinical strain RIGLD 4-151 was used. C. jejuni OMVs were isolated using ultracentrifugation and were analyzed by scanning electron microscopy (SEM) and dynamic light scattering (DLS). HT-29 human colon cancer epithelial cells were treated with OMVs for 24, 48, and 72 hours. Cell viability of HT-29 cells exposed to OMVs was measured by MTT assay.
Results: Our results showed that C. jejuni strain RIGLD 4-151 released round-shaped nanovesicles ranging from 10 to 250 nm. The cytotoxicity assays unveiled a dose-dependent reduction in cell viability following exposure to various concentrations of C. jejuni OMVs.
Conclusion: The present study demonstrated that OMVs derived from C. jejuni strain RIGLD 4-151 can significantly affect viability of HT-29 cells. Further research is required to elucidate the definite role of OMVs derived from C. jejuni strain RIGLD 4-151 in the pathogenesis of C. jejuni.
- Campylobacter jejuni
- Outer membrane vesicle
- Cell viability
- HT-29 cells
How to Cite
References
Kim SH, Chelliah R, Ramakrishnan SR, Perumal AS, Bang WS, Rubab M, Daliri EB, Barathikannan K, Elahi F, Park E, Jo HY. Review on stress tolerance in Campylobacter jejuni. Frontiers in cellular and infection microbiology. 2021 Feb 4;10:596570.
Xi D, Alter T, Einspanier R, Sharbati S, Gölz G. Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion. Gut pathogens. 2020 Dec;12:1-1.
Kreling V, Falcone FH, Kehrenberg C, Hensel A. Campylobacter sp.: Pathogenicity factors and prevention methods—new molecular targets for innovative antivirulence drugs?. Applied Microbiology and Biotechnology. 2020 Dec;104:10409-36.
Elmi A, Nasher F, Dorrell N, Wren B, Gundogdu O. Revisiting Campylobacter jejuni virulence and fitness factors: role in sensing, adapting, and competing. Frontiers in cellular and infection microbiology. 2021 Feb 3;10:607704.
Lopes GV, Ramires T, Kleinubing NR, Scheik LK, Fiorentini ÂM, da Silva WP. Virulence factors of foodborne pathogen Campylobacter jejuni. Microbial pathogenesis. 2021 Dec 1;161:105265.
Singh A, Khan A, Ghosh T, Mondal S, Mallick AI. Gut microbe-derived outer membrane vesicles: a potential platform to control cecal load of Campylobacter jejuni. ACS Infectious Diseases. 2021 Mar 16;7(5):1186-99.
Rezasoltani S, Sharafkhah M, Aghdaei HA, Olfatifar M, Mojarad EN, Yadegar A, Zali MR. The Association between Gut Microbiota and Intestinal Toll-like Receptors Genes Expression in Adenomatous and Colorectal Cancerous patients.
Antunes JC, Seabra CL, Domingues JM, Teixeira MO, Nunes C, Costa-Lima SA, Homem NC, Reis S, Amorim MT, Felgueiras HP. Drug targeting of inflammatory bowel diseases by biomolecules. Nanomaterials. 2021 Aug 10;11(8):2035.
Hameed A, Woodacre A, Machado LR, Marsden GL. An updated classification system and review of the lipooligosaccharide biosynthesis gene locus in Campylobacter jejuni. Frontiers in Microbiology. 2020 May 19;11:677.
Asiabar AS, Aghdaei HA, Zamani S, Bokaie S, Zali MR, Feizabadi MM. Molecular detection of Campylobacter jejuni in patients with Crohn’s disease in Iran. Medical Journal of the Islamic Republic of Iran. 2019;33:76.
Daulagala AC, Bridges MC, Kourtidis A. E-cadherin beyond structure: a signaling hub in colon homeostasis and disease. International journal of molecular sciences. 2019 Jun 5;20(11):2756.
Burnham PM, Hendrixson DR. Campylobacter jejuni: collective components promoting a successful enteric lifestyle. Nature Reviews Microbiology. 2018 Sep;16(9):551-65.
Taheri N, Fällman M, Wai SN, Fahlgren A. Accumulation of virulence-associated proteins in Campylobacter jejuni outer membrane vesicles at human body temperature. Journal of proteomics. 2019 Mar 20;195:33-40.
Mohan V. The role of probiotics in the inhibition of Campylobacter jejuni colonization and virulence attenuation. European Journal of Clinical Microbiology & Infectious Diseases. 2015 Aug;34:1503-13.
Macion A, Wyszyńska A, Godlewska R. Delivery of toxins and effectors by bacterial membrane vesicles. Toxins. 2021 Nov 26;13(12):845.
Talukdar PK, Negretti NM, Turner KL, Konkel ME. Molecular dissection of the Campylobacter jejuni CadF and FlpA virulence proteins in binding to host cell fibronectin. Microorganisms. 2020 Mar 11;8(3):389.
Jan AT. Outer membrane vesicles (OMVs) of gram-negative bacteria: a perspective update. Frontiers in microbiology. 2017 Jun 9;8:1053.
Singh A, Khan A, Ghosh T, Mondal S, Mallick AI. Naturally secreted bacterial outer membrane vesicles: potential platform for a vaccine against Campylobacter jejuni. bioRxiv. 2020 Oct 16:2020-10.
Furuyama N, Sircili MP. Outer membrane vesicles (OMVs) produced by gram‐negative bacteria: structure, functions, biogenesis, and vaccine application. BioMed Research International. 2021;2021(1):1490732.
Facciolà A, Riso R, Avventuroso E, Visalli G, Delia SA, Lagana P. Campylobacter: from microbiology to prevention. Journal of preventive medicine and hygiene. 2017 Jun;58(2):E79.
Davies C, Taylor AJ, Elmi A, Winter J, Liaw J, Grabowska AD, Gundogdu O, Wren BW, Kelly DJ, Dorrell N. Sodium taurocholate stimulates Campylobacter jejuni outer membrane vesicle production via down-regulation of the maintenance of lipid asymmetry pathway. Frontiers in cellular and infection microbiology. 2019 May 29;9:177.
Elmi A, Nasher F, Jagatia H, Gundogdu O, Bajaj‐Elliott M, Wren B, Dorrell N. Campylobacter jejuni outer membrane vesicle‐associated proteolytic activity promotes bacterial invasion by mediating cleavage of intestinal epithelial cell E‐cadherin and occludin. Cellular microbiology. 2016 Apr;18(4):561-72.
Elmi A, Dorey A, Watson E, Jagatia H, Inglis NF, Gundogdu O, Bajaj‐Elliott M, Wren BW, Smith DG, Dorrell N. The bile salt sodium taurocholate induces Campylobacter jejuni outer membrane vesicle production and increases OMV‐associated proteolytic activity. Cellular microbiology. 2018 Mar;20(3):e12814.
Konkel ME, Talukdar PK, Negretti NM, Klappenbach CM. Taking control: Campylobacter jejuni binding to fibronectin sets the stage for cellular adherence and invasion. Frontiers in Microbiology. 2020 Apr 9;11:564.
Kalluri R, Neilson EG. Epithelial-mesenchymal transition and its implications for fibrosis. The Journal of clinical investigation. 2003 Dec 15;112(12):1776-84.
Ellis TN, Kuehn MJ. Virulence and immunomodulatory roles of bacterial outer membrane vesicles. Microbiology and molecular biology reviews. 2010 Mar;74(1):81-94.
Bomberger JM, MacEachran DP, Coutermarsh BA, Ye S, O'Toole GA, Stanton BA. Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles. PLoS pathogens. 2009 Apr 10;5(4):e1000382.
Elmi A, Watson E, Sandu P, Gundogdu O, Mills DC, Inglis NF, Manson E, Imrie L, Bajaj-Elliott M, Wren BW, Smith DG. Campylobacter jejuni outer membrane vesicles play an important role in bacterial interactions with human intestinal epithelial cells. Infection and immunity. 2012 Dec;80(12):4089-98.
Lindmark B, Rompikuntal PK, Vaitkevicius K, Song T, Mizunoe Y, Uhlin BE, Guerry P, Wai SN. Outer membrane vesicle-mediated release of cytolethal distending toxin (CDT) from Campylobacter jejuni. BMC microbiology. 2009 Dec;9:1-0.
Kemper L, Hensel A. Campylobacter jejuni: targeting host cells, adhesion, invasion, and survival. Applied Microbiology and Biotechnology. 2023 May;107(9):2725-54.
Kemper L, Hensel A. Campylobacter jejuni: targeting host cells, adhesion, invasion, and survival. Applied Microbiology and Biotechnology. 2023 May;107(9):2725-54.
Linton D, Dorrell N, Hitchen PG, Amber S, Karlyshev AV, Morris HR, Dell A, Valvano MA, Aebi M, Wren BW. Functional analysis of the Campylobacter jejuni N‐linked protein glycosylation pathway. Molecular microbiology. 2005 Mar;55(6):1695-703.
Juodeikis R, Carding SR. Outer membrane vesicles: biogenesis, functions, and issues. Microbiology and Molecular Biology Reviews. 2022 Dec 21;86(4):e00032-22.
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