ITS2-rDNA fragments of Leishmania species isolated from the great gerbil in Iran, 2021
Novelty in Biomedicine,
Vol. 11 No. 1 (2023),
Background: The great gerbil (Rhombomys opimus), is widely distributed in Asia and is a natural reservoir for zoonotic cutaneous leishmaniasis in many endemic areas, as well as Iran.
Materials and Methods: In this study, infection to Leishmania species was investigated by two methods, parasitological and molecular survey, in the small number of R. opimus collected from Jovain, a Zoonotic Cutaneous Leishmaniasis (ZCL) focus located in North East of Iran.
Results: Parasitological observation showed infection in only one of five rodents. But, ITS2-Nested-PCR revealed Leishmania infection in three out of 5 gerbils, including the parasitological positive one. Based on the PCR amplified size, two cases of infections were Leishmania major and one Leishmania turanica, their sequences are accessible in GenBank. The results of sequence analysis were consistent with the results obtained based on the size of the PCR.
Conclusion: These findings re-confirm the important role of R. opimus in the natural circulation of Leishmania spp and indicate the need to be concerned about the disease in the study area.
- Iran, Leishmania turanica, Leishmania major, Nested-PCR, Rhombomys opimus
How to Cite
Bamorovat M, Sharifi I, Tavakoli Oliaee R, Jafarzadeh A, Khosravi A. Determinants of unresponsiveness to treatment in cutaneous leishmaniasis: a focus on anthroponotic form due to Leishmania tropica. Frontiers in Microbiology. 2021:1143.
Saraei M, Farash BR, Hajialilo E. Cutaneous leishmaniasis as an increasing threat for Iranian travellers attending religious ceremonies. Eastern Mediterranean Health Journal. 2021;27(1):90-5.
Hashiguchi Y, Nieto LV, Villegas NC, Gomez EA, Kato H. Topical Treatment of Cutaneous Leishmaniasis: A Case Treated with A Glucantime-Based Lotion Experienced in Ecuador and A Mini Review. Archives of Medical and Clinical Research. 2021;1:1-15.
Hajjaran H, Saberi R, Borjian A, et al. The geographical distribution of human cutaneous and visceral leishmania species identified by molecular methods in Iran: a systematic review with meta-analysis. Frontiers in Public Health. 2021;9:835.
World Health Organization. Leishmaniasis FactSheet. 2021. Available at https://www.who.int/news-room/fact-sheets/detail/leishmaniasis/ (Accessed 24 July 2022).
Mozafari O, Sofizadeh A, Shoraka HR. Distribution of Leishmania Infection in Humans, Animal Reservoir Hosts and Sandflies in Golestan Province, Northeastern Iran: A Systematic Review and Meta-Analysis. Iranian Journal of Public Health. 2020;49(12):2308.
Karimi A, Hanafi-Bojd AA, Yaghoobi-Ershadi MR, Akhavan AA, Ghezelbash Z. Spatial and temporal distributions of phlebotomine sand flies (Diptera: Psychodidae), vectors of leishmaniasis, in Iran. Acta tropica. 2014;132:131-9.
Jahanifard E, Hanafi-Bojd AA, Akhavan AA, Sharififard M, Khazeni A, Vazirianzadeh B. Modeling of at risk areas of Zoonotic Cutaneous Leishmaniasis (ZCL) using Hierarchical Analysis Process (AHP) and Geographic Information System (GIS) in Southwest of Iran. Journal of Entomological Research. 2020;44(2):315-22.
Sanei-Dehkordi A, Soleimani-Ahmadi M, Zare M, Mirzaei H. Epidemiological features of cutaneous leishmaniasis and distribution of sand flies in an endemic area in southeast of Iran. Parasite Epidemiology and Control. 2021;14:e00220.
Yaghoobi-Ershadi M. Phlebotomine sand flies (Diptera: Psychodidae) in Iran and their role on Leishmania transmission. Journal of arthropod-borne diseases. 2012;6(1):1.
Yaghoobi-Ershadi MR. Control of phlebotomine sand flies in Iran: a review article. Journal of arthropod-borne diseases. 2016;10(4):429.
Rassi Y, Sofizadeh A, Abai M, et al. Molecular detection of Leishmania major in the vectors and reservoir hosts of cutaneous leishmaniasis in Kalaleh District, Golestan Province, Iran. Journal of Arthropod-Borne Diseases. 2008;2(2):21-7.
Akhavan AA, Mirhendi H, Khamesipour A, et al. Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs. Experimental parasitology. 2010;126(4):552-556.
Parvizi P, Hedayati M. Leishmania infections in rodents, reservoir hosts of zoonotic cutaneous leismaniasis in turkemen sahara, gonbad, golastan province. 2010.
Sofizadeh A, Hanafi-Bojd A, Shoraka H. Modeling spatial distribution of Rhombomys opimus as the main reservoir host of zoonotic cutaneous leishmaniasis in northeastern Iran. Journal of vector borne diseases. 2018;55(4):297.
Asl FG, Mohebali M, Jafari R, et al. Leishmania spp. infection in Rhombomys opimus and Meriones libycus as main reservoirs of zoonotic cutaneous leishmaniasis in central parts of Iran: Progress and implications in health policy. Acta Tropica. 2022;226:106267.
Mohammadiha A, Dalimi A, Mahmoodi MR, Parian M, Pirestani M, Mohebali M. The PCR-RFLP-based detection and identification of the Leishmania species causing human cutaneous leishmaniasis in the Khorasan-Razavi Province, Northeast of Iran. Journal of Arthropod-Borne Diseases. 2017;11(3):383.
Yaghoobi Ershadi M, Akhavan A, Zahraei Ramazani A, et al. Epidemiological study in a new focus of cutaneous leishmaniasis in the Islamic Republic of Iran. EMHJ-Eastern Mediterranean Health Journal;9 (4):816-26.
). Wikidata. Available at https://www.wikidata.org/wiki/Q2610777/. (Accessed August 2022 ).
Darvish J, Siah SR, Mirshamsi Ko, Keyvan FN, Hashemi N, Sadeghi SF. Diversity of the rodents of northeastern Iran. 2006.
Sambrook J GMMCAlmCSHLP, New York, pp 2028
Akhavan A, Yaghoobi-Ershadi M, Khamesipour A, et al. Dynamics of Leishmania infection rates in Rhombomys opimus (Rodentia: Gerbillinae) population of an endemic focus of zoonotic cutaneous leishmaniasis in Iran. Bulletin de la Société de pathologie exotique. 2010;103(2):84-9.
Mohebali M, Javadian E, Yaghoobi Ershadi M, Akhavan A, Hajjaran H, Abaei M. Characterization of Leishmania infection in rodents from endemic areas of the Islamic Republic of Iran. EMHJ-Eastern Mediterranean Health Journal. 2004;10(4-5):591-9.
Parvizi P, Moradi G, Akbari G, et al. PCR detection and sequencing of parasite ITS-rDNA gene from reservoirs host of zoonotic cutaneous leishmaniasis in central Iran. Parasitology research. 2008;103(6):1273-8.
Moreira M, Luvizotto MCR, Garcia JF, Corbett C, Laurenti M. Comparison of parasitological, immunological and molecular methods for the diagnosis of leishmaniasis in dogs with different clinical signs. Veterinary parasitology. 2007;145(3-4):245-52.
Aghamolaei S, Behniafar H, Behravan M, Hajjaran H, Moin Vaziri V. Probability of false-negative results in microscopical detection of cutaneous leishmaniasis: more accurate screening by kDNA-PCR during epidemiological survey. Journal of Parasitic Diseases. 2020;44(4):781-784.
Schönian G, Nasereddin A, Dinse N, et al. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagnostic microbiology and infectious disease. 2003;47(1):349-358.
Hajjaran H, Mohebali M, Abaei MR, et al. Natural infection and phylogenetic classification of Leishmania spp. infecting Rhombomys opimus, a primary reservoir host of zoonotic cutaneous leishmaniasis in northeast Iran. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2013;107(9):550-557.
- Abstract Viewed: 30 times
- pdf Downloaded: 125 times