Apoptosis Induction by Lactobacillus casei Acidic Proteins in the Colorectal Cancer Cell Line
Novelty in Biomedicine,
Vol. 10 No. 4 (2022),
Background: Colorectal cancer (CRC) ranks third in cancer prevalence. Lactobacillus casei, a probiotic
bacterium, can optimize the microbiota population of the gastrointestinal tract and prevent the growth of harmful bacteria that induce carcinogenesis. In this study, we investigated the effect of L. casei acidic proteins on apoptosis in the SW480 cell line to identify a drug protein for treating CRC.
Materials and Methods: We assayed the effect of the isolated acid-resistant proteins of the Chaperonin
bacterium, a metal-dependent Hydrolase, and Lysozyme on the SW480 colorectal cancer cell line apoptosis
pathway gene expression with a Real-Time RT-PCR.
Results: All three proteins induced apoptosis in the cells treated separately with each of the proteins. The
results showed that the up-regulation of BAX and P53 gene expression and the apoptosis pathway were
significantly induced. Also, BCL2 expression was down-regulated, and significant anti-apoptotic was observed
at p<0.0001. Moreover, the cells treated with these three proteins down-regulated the expression of MAP2K1
and provoked the opposite apoptosis pathway.
Conclusion: Our results found that these proteins would be a good choice for potential CRC treatment.
- Colorectal cancer, Lactobacillus casei, Acid-resistant proteins, Apoptosis
How to Cite
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