CRISPR-Cas9 Mediated Capsule Gene Silencing in Escherichia coli
Novelty in Biomedicine,
Vol. 6 No. 4 (2018),
31 October 2018
,
Page 162-166
https://doi.org/10.22037/nbm.v6i4.21672
Abstract
Background: Genomic engineering of Escherichia coli is applied to design and produce recombinant proteins as the new drugs. The aim of this study was to CRISPR-Cas9 mediated capsule gene silencing in E. coli.Materials and Methods: We suppressed genes involved in capsule expression of E.coli by CRISPR cas9 process. The constructed E.coli was confirmed by microscopic smear, transmission electron microscopy and T7 phage influence assay.
Results: The results were shown that the inhibition of capsule production was carried out successfully and there was not any capsule layer around the bacteria.
Conclusion: E. coli without any capsule around may proper for replacement of it with other molecules in future.
- capsule
- CEISPR
- Escherichia coli
- TEM
How to Cite
References
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