The Inhibitory Effects of Lactobacillus Reuteri’s Cell Wall on Cell Proliferation in the HCT-116 Colorectal Cancer Cell Line
Novelty in Biomedicine,
Vol. 6 No. 3 (2018),
7 Mordad 2018
,
Page 126-130
https://doi.org/10.22037/nbm.v6i3.19415
Abstract
Background: Colorectal cancer (CRC) is the third and a second common cancer in men and women respectively in the world and about 1.4 million new cases diagnosed in 2012. The normal gut microflora consists of bacterial species. One group of them is probiotics, which confer a health benefit to the host. Lactobacillus reuteri (L.reuteri) is known as a probiotic, which lead to the prevention of colorectal cancer. The aim of this study was to assess the inhibitory effects of Lactobacillus reuteri’s cell wall on cell proliferation in the colorectal cancer HCT-116 cell line.Materials and Methods: The cells of HCT-116 cell line were grown at 37ᵒC, 5% CO2. L.reuteri was obtained from the Iranian Biological Resource Center and cultured in the MRS Broth at 37ᵒC for 48h anaerobically. The cell wall was prepared by the freezing-thawing procedure. So the inhibitory effect of L.reuteri on the growth and proliferation of HCT-116 cells was assessed by MTT assay.
Results: The cell wall from L.reuteri inhibited cell proliferation on colorectal cancer HCT-116 cell line. It showed dose- and –time dependent inhibition.
Conclusion: These results demonstrated that cell wall of L.reuteri inhibits cell proliferation of HCT-116 cell line.- Colorectal cancer
- Lactobacillus reuteri
- HCT-116 cell line
How to Cite
References
Kostic AD, Gevers D, Pedamallu CS, Michaud M, Duke F, Earl, AM, et al. Genomic analysis identifies association of Fusobacterium with colorectal carcinoma. Genome Res. 2012;22(2):292–8.
- Tenesa A, Dunlop MG. New insights into the aetiology of colorectal cancer from genome – wide association studies. Nat Rev Gent. 2009;10(6):353–8.
Garagnani P, Pirazzini C, Franceschi C. Colorectal cancer microenvironment: among nutrition, gut microbiota, inflammation and epigenetics. Curr Pharm Des. 2013;19:765–78.
Wickham R, Lassere Y, editors. The ABCs of colorectal cancer. Seminars in oncology nursing. 2007.
Siegel RL, Miller KD, Jemal A. Cancer statistics, 2017. CA Cancer J Clin. 2017;67:7-30.
Fakheri H, Janbabai G, Bari Z, Eshqi F. The Epidemiologic and Clinical-Pathologic Characteristics of Colorectal Cancers from 1999 to 2007 in Sari, Iran. JMUMS; 2008.18(67).
Larsson SC, Wolk A. Meat consumption and risk of colorectal cancer: a meta-analysis of prospective studies. Int J Cancer. 2006;119:2657-2664.
Riboli E, Norat T. Epidemiologic evidence of the protective effect of fruit and vegetables on cancer risk. Am J Clin Nutr. 2003;78:559-69.
Haggar FA, Boushey RP. Colorectal cancer epidemiology: incidence, mortality, survival, and risk factors. Clin Colon Rectal Surg. 2009;22:191-7.
Rowland IR. The role of the gastrointestinal microbiota in colorectal cancer. Curr Pharm Des. 2009;15:1524-7.
- Zhu Q, Gao R, Wu W, Qin H. The role of gut microbiota in the pathogenesis of colorectal cancer. Tumour Biol. 2013;34:1285-1300.
Sanges M, Valente G, Rea M, Della Gatta R, De Franchis G, Sollazzo R, et al. Probiotics in spondyloarthropathy associated with ulcerative colitis: a pilot study. Eur Rev Med Pharmacol Sci. 2009;13(3):233–4.
Akbari A, Mobini GR, Maghsoudi R, Akhtari J, Faghihloo E, Farahnejad Z. Modulation of transforming growth factor β signaling transducers in colon adenocarcinoma cells induced by staphylococcal enterotoxin B. 2016;13(1):909-14.
Chen CC, Lin WC, Kong MS, Shi HN, Walker WA, Lin CY, et al. Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumor growth both in segmental orthotopic colon cancer and extra – intestinal tissue. Br J Nutr. 2011;30:1–12.
Armaghany T, Wilson JD, Chu Q, Mills G. Genetic Alterations in Colorectal Cancer. Gastrointest Cancer Res. 2012;5(1):19–27.
Riki M, Farokhi F, Tukmechi A. The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line. TUMJ. 2013;70(11):691-9.
Riki M, Farokhi F, Tukmechi A. The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line. TUMJ. 2013;70(11):691-9.
KimY, Lee D, Kim D, Cho J, Yang J, Chung M, et al. Inhibition of proliferation in colon cancer cell lines and harmful enzyme activity of colon bacteria by Bifidobacterium adolescentis SPM0212. Arch Pharm Res. 2008;31:468-73.
Angeliki Tiptiri-Kourpeti, Katerina Spyridopoulou, Valentina Santarmaki1,Georgios Aindelis, Evgenia Tompoulidou amd et al. Lactobacillus casei Exerts Anti-Proliferation Effects Accompanied by Apoptotic Cell Death and Up-Regulation of TRAIL in Colon Carcinoma Cells. 2016.
Mohammad Mehdi Soltan Dallal, Majid Mojarrad, Fatemeh Baghbani, Reza Raoofian, Jalal Mardaneh, Zohreh Salehipour. Effects of probiotic Lactobacillus acidophilus and Lactobacillus casei on colorectal tumor cells activity (CaCo-2). Arch Iran Med. 2015;18(3):167-72.
Ying WAN, Yixin, Cuilizhang, Dachang WU, Dapeng DING, Li TANG, et al. Fermentation supernatants of Lactobacillus delbrueckii inhibit growth of human colon cancer cells and induce apoptosis through a caspase 3 dependent pathway. ONCOLOGY LETTERS. 2014;7:1738-42.
Mahmoudi Aslzadeh H., Fazeli M., Eaidi A., Samadi N., Jamalifar H. and Parsaseresht L. Study of probiotic effect of Bifidobacterium bifidum on CacoII cancer cell line. Journal of Molecular And Cellular Research (Iranian Journal of Biology). 2013;378-85.
Orlando A, Messa C, Linsalata M, Cavallini A, Russo F. Effects of Lactobacillus rhamnosus GG on proliferation and polyamine metabolism in HGC-27 human gastric and DLD-1 colonic cancer cell lines. Immunopharmacol Immunotoxicol. 2009;31:108-16.
Riki M, Farokhi F, Tukmechi A. The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line. TUMJ. 2013;70(11):691-9.
- Abstract Viewed: 417 times
- PDF Downloaded: 263 times