Seroprevalence Survey of Visceral Leishmaniasis among Children up to 12 Years old and Domestic Dogs in Rural Areas of Dehloran District, Ilam Province of West Part of Iran, 2014
Novelty in Biomedicine,
Vol. 5 No. 2 (2017),
24 April 2017
AbstractBackground: Visceral leishmaniasis (VL), caused by Leishmania infantum (L. infantum), is a life-threatening vector-borne parasitic disease is distributed in some parts of the world. The disease is endemic in some parts of Iran. This study was aimed to determine the seroprevalence of VL among children and domestic dogs (as a reservoir of the parasite) in Dehloran, west of Iran.
Materials and Methods: This cross-sectional study was carried out in Dehloran County. The blood samples of 872 children up to 12 years old and 52 dogs were collected from 10 villages of Dehloran using randomly-clustered sampling method. Sera were separated from all peripheral blood samples and tested by direct agglutination test (DAT). Anti-Leishmania infantum antibodies at titers of ≥1:800 and ≥1:80 were considered as Leishmania infantum infection in human and dog, respectively.
Results: In general, among 872 human samples, 1.03% of samples had anti-Leishmania antibody with 1:1600 titers and 1.26% had 1:800 titers. In addition, from 52 dog samples, 21.15% of dogs had a titer of ≥1:320 and 25% had 1:80 and 1:160 titers.
Conclusion: Our findings indicate that the seropositive dogs in the studied areas are considerable and L. infantum may be circulated between human and domestic dog in the studied area. Further study of isolation and molecular identification of Leishmania spp. is recommended.
- Visceral Leishmaniasis
- Direct Agglutination Test
How to Cite
Ardehali S, Rezaei H, Nadim A. Leishmania Parasite and leishmaniasis: Tehran: Tehran University Publishment Center; 1994.
Roberts L, Janovy J. Foundation of parasitology, 5th. WCB Company, UK. 2000:347-410.
Godal T, Ozcel A, Alkan M. New dimension for parasaitology in the 21st century. parasitology for 21st century CAB International. 1996:1-13.
Desjeux P. The increase in risk factors for leishmaniasis worldwide. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2001;95(3):239-43.
Organization WH. Working to overcome the global impact of neglected tropical diseases: first WHO report on neglected tropical diseases. 2010.
Organization WH. The leishmaniases: report of a WHO expert committee [meeting held in Geneva from 10 to 16 November 1982]. 1984.
Mohebali M. Epidemiological status of visceral leishmaniasis in Iran: experiences and review of literature. Journal of Clinical & Experimental Pathology. 2013;2012.
Mohebali M. Visceral leishmaniasis in Iran: review of the epidemiological and clinical features. Iranian journal of parasitology. 2013;8(3):348.
Mohebali M, Edrissian G, Nadim A. Application of direct agglutination test (DAT) for the diagnosis and seroepide-miological studies of visceral leishmaniasis in Iran. Iranian journal of parasitology. 2006;1(1):15-25.
Shirzadi M, Esfahania S, Mohebalia M, et al. Epidemiological status of leishmaniasis in the Islamic Republic of Iran, 1983–2012. EMHJ. 2015;21(10).
Moshfe A, Mohebali M, Edrissian G, et al. Seroepidemiological study on canine visceral leishmaniasis in Meshkin-Shahr district, Ardabil province, northwest of Iran during 2006-2007. Iranian Journal of Parasitology. 2008;3(3):1-10.
Fakhar M, Motazedian MH, Asgari Q, Kalantari M. Asymptomatic domestic dogs are carriers of Leishmania infantum: possible reservoirs host for human visceral leishmaniasis in southern Iran. Comp. Clin. Path. 2012;21(5):801-7.
Fakhar M, Kia AA, Gohardehi S, et al. Emergence of a new focus of visceral leishmaniasis due to Leishmania infantum in Golestan Province, north-eastern of Iran. J. Parasit. Dis. 2014;38(3):255-9.
Mohebali M, Hamzavi Y, Edrissian GH, Forouzani A. Seroepidemiological study of visceral leishmaniasis among humans and animal reservoirs in Bushehr province, Islamic Republic of Iran. 2001.
Abbaszadeh-Afshar M, Mohebali M, Sharifi I, et al. Seroepidemiological survey of Visceral leishmaniasis among nomadic tribes of Kerman Province, Southeastern Iran: An observational study for implication to health policy. Journal of Biostatistics and Epidemiology. 2015;1(2):105-11.
Rakhshanpour A, Mohebali M, Akhondi B, Rahimi MT, Rokni MB. Serological Survey and Associated Risk Factors of Visceral Leish-maniasis in Qom Province, Central Iran. Iranian journal of public health. 2014;43(1):50.
Fakhar M, Mohebali M, Barani M. Identification of Endemic Focus of Kala ـ azar and Seroepidemiologcial Study of Visceral Leishmania Infection in Human and Canine in Qom Province, Iran. Armaghane danesh. 2004;9(1):43-52.
Torabi V, Mohebali M, Edrissian G, et al. Seroepidemiological survey of visceral leishmaniasis by direct agglutination test in Bojnoord district, north Khorasan province in 2007. Iranian Journal of Epidemiology. 2009;4(3):43-50.
GhH E, Hajjaran H, Mohebali M, Soleimanzadeh G, Bokaei S. Application and evaluation of direct agglutination test in ser-diagnosis of visceral leishmaniasis in man and canine reservoirs in Iran. Iranian J Med Sci. 1996;21:119-24.
EDRISIAN GH, Nadim A, Alborzi A, Aredehali S. Visceral leishmaniasis; the Iranian experience. 1998.
Mohebali M, Fotouhi A, Hooshmand B, et al. Comparison of miltefosine and meglumine antimoniate for the treatment of zoonotic cutaneous leishmaniasis (ZCL) by a randomized clinical trial in Iran. Acta tropica. 2007;103(1):33-40.
Nadim A, Javadian E, Mohebali M, Momeni A. Leishmania parasite and leishmaniasis. Markaze Nashre Daneshgahi Publ. 2008.
Sundar S, Singh A, Rai M, et al. Efficacy of miltefosine in the treatment of visceral leishmaniasis in India after a decade of use. Clin. Infect. Dis. 2012:cis474.
Hailu A. Pre-and post-treatment antibody levels in visceral leishmaniasis. Trans. R. Soc. Trop. Med. Hyg. 1990;84(5):673-675.
Ozbel Y, Oskam L, Ozensoy S, et al. A survey on canine leishmaniasis in western Turkey by parasite, DNA and antibody detection assays. Acta Tropica. 2000;74(1):1-6.
EDRISIAN GH, Hajaran H, Mohebali M, Soleimanzaeh G, Bokaei S. Application and evaluation of direct agglutination test in sero-diagnosis of visceral leishmaniasis in man and canine reservoirs in Iran. 1996.
Mohebali M, Hajjaran H, Hamzavi Y, et al. Epidemiological aspects of canine visceral leishmaniosis in the Islamic Republic of Iran. Veterinary parasitology. 2005;129(3):243-51.
Jahangir A, Akhoundi B, Mohebali M, Ghaderipour A, Kakoee Z, Najafi F. Seroepidemiological Survey of Human Visceral Leishmaniasis in Ilam Province, West of Iran In 2013. Iranian journal of parasitology. 2015;10(1):56.
Www.WHO.int. Leishmaniasis. 2013.
Chegeni SA, Ourmazdi H, Mohebali M, Akhlaghi L, Sharafi M, Akhoundi B. Seroepidemiological study of visceral leishmaniasis (Human infection) in east Myankooh area, in Lorestan province by Direct Agglutination Test (DAT).
Kavarizadeh F, Vazirianzadeh B, Rassi Y, Jalali Glusang A, Moravvej SA. A faunistic study of sand flies of Musian District, Southwestern of Iran. Pakistan J Zool. 2013;45(2):549-54.
Vahabi A, Rassi Y, Oshaghi M, Sayyadi M, Rafizadeh S. Detection of Leishmania major DNA within wild caught Phlebotomus papatasi and species composition of sand flies in endemic focus of cutaneous leishmaniasis, in western Iran. Journal of Parasitic Diseases.1-6.
Mahmoudvand H, Mohebali M, Sharifi I, et al. Epidemiological aspects of visceral leishmaniasis in Baft District, Kerman province, southeast of Iran. Iranian journal of parasitology. 2011;6(1):1.
Arshi S, Mohebali M, Akhoundi B, et al. Idendification of a new endemic focus of kala-azar and seroepidemiological study of visceral Leishmania infection in Ardebil province. 2002.
Edrissian G, Hafizi A, Afshar A, Soleiman-Zadeh G, Movahed-Danesh A, Garoussi A. An endemic focus of visceral leishmaniasis in Meshkin-Shahr, east Azerbaijan province, north-west part of Iran and IFA serological survey of the disease in this area. Bulletin de la societé de pathologie exotique et de ses filiales. 1987;81(2):238-48.
Fakhar M, Rahmati B, Gohardehi S, et al. Molecular and seroepidemiological survey of visceral leishmaniasis among humans and domestic dogs in Mazandaran Province, North of Iran. Iranian journal of parasitology. 2011;6(4):51.
Avizeh R, Mohebali M, Sheikholslami M. Seroepidemiological investigation of visceral leishmaniasis in dogs of Ahvaz district, Iran. Archives of Razi Institute. 2007;62(1):31-7.
Malmasi A, Janitabar S, Mohebali M, et al. Seroepidemiologic survey of canine visceral leishmaniasis in Tehran and Alborz Provinces of Iran. Journal of arthropod-borne diseases. 2014;8(2):132.
Haddadzade H, Fattahi R, Mohebali M, Akhoundi B, Ebrahimzade E. Seroepidemiologcal investigation of visceral leishmaniasis in stray and owned dogs in Alborz province, Central Iran using direct agglutination test. Iranian journal of parasitology. 2013;8(1):152.
Bamorovat M, Sharifi I, Mohammadi MA, et al. Canine Visceral Leishmaniasis in Kerman, Southeast of Iran: A Seroepidemiological, Histopathological and Molecular Study. Iranian journal of parasitology. 2014;9(3):342.
Bokai S, Mobedi I, Edrissian GhH NA. Seroepidemiological study of canine visceral leishmaniasis in Meshkin-Shahr, northwest of Iran. Arch Inst Razi. 1998;41(6):48-9.
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