An investigation into the effect of eight weeks of aerobic exercise with orange juice consumption on antioxidant capacity and inflammatory factors in overweight middle-aged women
Researcher Bulletin of Medical Sciences,
Vol. 29 No. 1 (2024),
4 Dey 2025
,
Page e14
Abstract
Objective: Overweight and obesity are among the most important public health concerns associated with several negative health outcomes. Several studies have found that the positive impact of orange juice may be due to the presence of its antioxidants, especially flavonoids and vitamin C. Vitamin C as an antioxidant is capable of neutralizing water-soluble ROS. Therefore, this study aimed to investigate the effect of eight weeks of aerobic exercise with orange juice extract on antioxidant capacity and inflammatory factors in overweight women.
Materials and Methods: A total of 60 overweight women participated in this study voluntarily. The research sample was selected using a random sampling method. They were then randomly divided into 4 groups. The treadmill endurance training protocol was carried out with a run-walk program for eight weeks, three sessions per week. All data were then entered into SPSS 24 to be analyzed.
Results: The results obtained by this study showed that eight weeks of aerobic exercise with orange juice consumption had no significant effect on high-sensitivity C-reactive protein (hs-CRP) levels and total antioxidant capacity in overweight middle-aged women, but eight weeks of aerobic exercise with orange juice consumption had a significant effect on malondialdehyde (MDA) levels and VO2max amount in overweight middle-aged women (P= 0.001).
Conclusion: Statistical studies showed that eight weeks of aerobic exercise with orange juice consumption causes a reduction in MDA levels, and orange juice consumption combined with aerobic exercise can have a synergistic effect compared to aerobic exercise alone and increase the effect of exercise on the oxidative stress suppression
- Aerobic exercise; Orange juice; Antioxidant capacity;, Inflammatory factors; Middle-aged women, Overweight
How to Cite
References
-Jakicic JM, Rogers RJ, Davis KK, Collins KA. Role of Physical Activity and Exercise in Treating Patients with Overweight and Obesity. Clinical chemistry. 2018;64(1):99-107.
-Douglas CC, Norris LE, Oster RA, Darnell BE, Azziz R, Gower BA. Difference in dietary intake between women with polycystic ovary syndrome and healthy controls. Fertility and sterility. 2006;86(2):411-7.
-Matsuda M, Shimomura I. Increased oxidative stress in obesity: implications for metabolic syndrome, diabetes, hypertension, dyslipidemia, atherosclerosis, and cancer. Obesity research & clinical practice. 2013;7(5):e330-e41.
-Kandaraki E, Chatzigeorgiou A, Livadas S, Palioura E, Economou F, Koutsilieris M, et al. Endocrine disruptors and polycystic ovary syndrome (PCOS): elevated serum levels of bisphenol A in women with PCOS. The Journal of Clinical Endocrinology & Metabolism. 2010;96(3):E480-E4.
-Teixeira Filho FL, Baracat EC, Lee TH, Suh CS, Matsui M, Chang RJ, et al. Aberrant expression of growth differentiation factor-9 in oocytes of women with polycystic ovary syndrome. The Journal of Clinical Endocrinology & Metabolism. 2002;87(3):1337-44.
-González F, Rote NS, Minium J, Kirwan JP. Reactive oxygen species-induced oxidative stress in the development of insulin resistance and hyperandrogenism in polycystic ovary syndrome. The Journal of Clinical Endocrinology & Metabolism. 2006;91(1):336-40.
-Kuşçu NK, Var A. Oxidative stress but not endothelial dysfunction exists in non-obese, young group of patients with polycystic ovary syndrome. Acta obstetricia et gynecologica Scandinavica. 2009;88(5):612-7.
-Repaci A, Gambineri A, Pasquali R. The role of low-grade inflammation in the polycystic ovary syndrome. Molecular and cellular endocrinology. 2011;335(1):30-41.
- Shayari Miri, Kiani F, Shaye Miri, Maleki F, Ahmadi M, Shvani M. The Prevalence of Polycystic Ovary Syndrome in Iranian Women: A Review and Meta-Analysis. Iranian Women's Midwifery and Infertility Journal. Oct. 1393. 115 (17), 21-11
-Taghavi SA, Bazarganipour F, Montazeri A, Kazemnejad A, Chaman R, Khosravi A. Health-related quality of life in polycystic ovary syndrome patients: A systematic review. Iranian journal of reproductive medicine. 2015;13(8):473.
-Bullon P, Newman HN, Battino M. Obesity, diabetes mellitus, atherosclerosis and chronic periodontitis: a shared pathology via oxidative stress and mitochondrial dysfunction? Periodontology 2000. 2014;64(1):139-53.
-Kurdoglu Z, Ozkol H, Tuluce Y, Koyuncu I. Oxidative status and its relation with insulin resistance in young non-obese women with polycystic ovary syndrome. Journal of endocrinological investigation. 2012;35(3):317-21.
-Mohamadin AM, Habib FA, Elahi TF. Serum paraoxonase 1 activity and oxidant/antioxidant status in Saudi women with polycystic ovary syndrome. Pathophysiology. 2010;17(3):189-96.
-Rochette L, Zeller M, Cottin Y, Vergely C. Diabetes, oxidative stress and therapeutic strategies. Biochimica et Biophysica Acta (BBA)-General Subjects. 2014;1840(9):2709-29.
-Yeon Lee J, Baw C-K, Gupta S, Aziz N, Agarwal A. Role of oxidative stress in polycystic ovary syndrome. Current Women's Health Reviews. 2010;6(2):96-107.
-Liu Y, Palanivel R, Rai E, Park M, Gabor TV, Scheid MP, et al. Adiponectin stimulates autophagy and reduces oxidative stress to enhance insulin sensitivity during high-fat diet feeding in mice. Diabetes. 2015;64(1):36-48.
-Chakraborty P, Ghosh S, Goswami S, Kabir SN, Chakravarty B, Jana K. Altered trace mineral milieu might play an aetiological role in the pathogenesis of polycystic ovary syndrome. Biological trace element research. 2013;152(1):9-15.
-Madnani N, Khan K, Chauhan P, Parmar G. Polycystic ovarian syndrome. Indian Journal of Dermatology, Venereology, and Leprology. 2013;79(3):310.
-Pigny P, Gorisse E, Ghulam A, Robin G, Catteau-Jonard S, Duhamel A, et al. Comparative assessment of five serum antimüllerian hormone assays for the diagnosis of polycystic ovary syndrome. Fertility and sterility. 2016;105(4):1063-9. e3.
-Zhang J, Zhang Y, Liu H, Bai H, Wang Y, Jiang C, et al. Antioxidant properties of high-density lipoproteins are impaired in women with polycystic ovary syndrome. Fertility and sterility. 2015;103(5):1346-54.
-Kurowska EM, Spence JD, Jordan J, Wetmore S, Freeman DJ, Piché LA, et al. HDL-cholesterol-raising effect of orange juice in subjects with hypercholesterolemia. The American journal of clinical nutrition. 2000;72(5):1095-100.
-Legro RS, Arslanian SA, Ehrmann DA, Hoeger KM, Murad MH, Pasquali R, et al. Diagnosis and treatment of polycystic ovary syndrome: an Endocrine Society clinical practice guideline. The Journal of Clinical Endocrinology & Metabolism. 2013;98(12):4565-92.
-Bayram F, Kocer D, Ozsan M, Muhtaroglu S. Evaluation of endothelial dysfunction, lipid metabolism in women with polycystic ovary syndrome: relationship of paraoxonase 1 activity, malondialdehyde levels, low-density lipoprotein subfractions, and endothelial dysfunction. Gynecological Endocrinology. 2012;28(7):497-501.
-Gaweł S, Wardas M, Niedworok E, Wardas P. Malondialdehyde (MDA) as a lipid peroxidation marker. Wiadomosci lekarskie (Warsaw, Poland: 1960). 2004;57(9-10):453-5.
-Fedewa MV, Hathaway ED, Higgins S, Forehand RL, Schmidt MD, Evans EM. Moderate, but not vigorous, intensity exercise training reduces C-reactive protein. Acta cardiologica. 2017:1-8.
-Rekha C, Poornima G, Manasa M, Abhipsa V, Devi JP, Kumar VH, Kekuda TR. Ascorbic acid, total phenol content and antioxidant activity of fresh juices of four ripe and unripe citrus fruits. Chemical Science Transactions. 2012;1(2):303-10.
-Fisher G, Hyatt TC, Hunter GR, Oster RA, Desmond RA, Gower BA. Effect of diet with and without exercise training on markers of inflammation and fat distribution in overweight women. Obesity. 2011 Jun 1;19(6):1131-6.
-Koh Y, Park K-S. Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals. Journal of exercise rehabilitation. 2017;13(4):472.
-Stickland MK, Petersen SR, Bouffard M. Prediction of maximal aerobic power from the 20-m multi-stage shuttle run test. Canadian Journal of Applied Physiology. 2003; 28(2):272-82.
-Golbidi S, Badran M, Laher I. Antioxidant and anti-inflammatory effects of exercise in diabetic patients. Experimental diabetes research. 2011.
-Pearson TA, Mensah GA, Alexander RW, Anderson JL, Cannon RO, Criqui M, et al. Markers of inflammation and cardiovascular disease: application to clinical and public health practice: a statement for healthcare professionals from the Centers for Disease Control and Prevention and the American Heart Association. Circulation. 2003; 107(3): 499-511.
-Moylan S, Eyre HA, Maes M, Baune BT, Jacka FN, Berk M. Exercising the worry away: how inflammation, oxidative and nitrogen stress mediates the beneficial effect of physical activity on anxiety disorder symptoms and behaviours. Neuroscience & Biobehavioral Reviews. 2013. 37(4):573-84.
-Teixeira-Lemos E, Nunes S, Teixeira F, Reis F. Regular physical exercise training assists in preventing type 2 diabetes development: focus on its antioxidant and anti-inflammatory properties. Cardiovascular diabetology. 2011; 10(1): 12.
-Eklund C, Jahan F, Pessi T, Lehtimäki T, Hurme M. Interleukin 1B gene polymorphism is associated with baseline C‐reactive protein levels in healthy individuals. European cytokine network. 2003; 14(3): 168-71.
- Zare Asma, Gaini Abbas Ali, Mogharnasi Mehdi et al. Comparison of two methods of continuous and periodic aerobic exercise on hs-CRP and blood lipids as predictors of cardiovascular disease. Journal of North Khorasan University of Medical Sciences. 2011. Volume 3. Number 4. Pages: 42-35.
-Nassis GP, Papantakou K, Skenderi K, Triandafillopoulou M, Kavouras SA, Yannakoulia M, et al. Aerobic exercise training improves insulin sensitivity without changes in body weight, body fat, adiponectin, and inflammatory markers in overweight and obese girls. Metabolism-Clinical and Experimental. 2005; 54(11): 1472-9.
- Abolfathi Farzaneh, Ranjbar Ruhollah, Shakrian Saeed et al. The effect of eight weeks of aerobic interval training on serum adiponectin, lipid profile and hs-CRP levels in women with type 2 diabetes. Journal of Endocrinology and Metabolism. 2015. Volume 17. Number 4. Pages: 324-316.
-Zoppini G, Targher G, Zamboni C, Venturi C, Cacciatori V, Moghetti P, et al. Effects of moderate-intensity exercise training on plasma biomarkers of inflammation and endothelial dysfunction in older patients with type 2 diabetes. Nutrition, Metabolism and Cardiovascular Diseases. 2006; 16(8): 543-9.
- Heidarianpour Ali, Samavati Sharif Mohammad Ali, Keshvari Maryam et al. And white blood cells in C Comparison of three exercise methods on the protein concentration of reactive patients with type 2 diabetes. Journal of Exercise Physiology and Physical Activity. 2016. No. 18. Page: 1384-1375.
-Piestrzeniewicz K, Łuczak K, Komorowski J, Jankiewicz-Wika J, Goch JH. Relation of C-reactive protein to obesity, adipose tissue hormones and cardiovascular risk factors in men treated with early percutaneous intervention in course of acute myocardial infarction. Neuro endocrinology letters. 2007; 28(4): 427-32.
-Balducci S, Zanuso S, Nicolucci A, Fernando F, Cavallo S, Cardelli P, et al. Anti-inflammatory effect of exercise training in subjects with type 2 diabetes and the metabolic syndrome is dependent on exercise modalities and independent of weight loss. Nutrition, Metabolism and Cardiovascular Diseases. 2010; 20(8): 608-17.
- Davison G, Gleeson M. Influence of acute vitamin C and/or carbohydrate ingestion on hormonal, cytokine, and immune responses to prolonged exercise. International journal of sport nutrition and exercise metabolism. 2005; 15(5):465-79.
-Davison G, Gleeson M. The effect of 2 weeks’ vitamin C supplementation on immunoendocrine responses to 2.5 h cycling exercise in man. European journal of applied physiology. 2006; 97(4):454-61.
-Devaraj S, Jialal I. Alpha tocopherol supplementation decreases serum C-reactive protein and monocyte interleukin-6 levels in normal volunteers and type 2 diabetic patients. Free Radical Biology and Medicine. 2000; 29(8):790-2.
-Metin G, Gumustas MK, Uslu E, Belce A, Kayserilioglu A. Effect of regular training on plasma thiols, malondialdehyde and carnitine concentrations in young soccer players. Chinese Journal of Physiology. 2003; 46(1):35-9.
- Yahya Zadeh Mohammad Amin, Mirsouri Rahim. The effect of eight weeks of moderate intensity aerobic exercise and omega-3 supplementation on total antioxidant capacity and hydrogen peroxide in young untrained men. complementary medicine. 2015. Number 3. Pages: 1289-1283.
- Naghizadeh Hassan, Ban Parvari Maryam, Salehi Kia Abbas. The effect of exercise program with vitamin E consumption on antioxidant status and cardiovascular risk factors. Zahedan Journal of Medical Research. 2009. Volume 12. Number 1. Pages: 39-33.
-Chrysohoou C, Panagiotakos DB, Pitsavos C, Skoumas I, Papademetriou L, Economou M, Stefanadis C. The implication of obesity on total antioxidant capacity in apparently healthy men and women: the ATTICA study. Nutrition, Metabolism and Cardiovascular Diseases. 2007; 17(8):590-7.
- Azamian Akbar, the glory of Reyhane. The effect of eight weeks of combined exercise on oxidative stress and lipid peroxidation in postmenopausal women with type 2 diabetes. Scientific Research Journal of Shahid Sadoughi University of Medical Sciences, Yazd. 2016. Volume 24. Number 8. Pages: 678-667.
-Yasribi Seyed Mohammad Ali, Salami Fatemeh, Rajabi Hamid et al. The effect of 8 weeks of speed training with and without vitamin E and C supplementation on plasma malondialdehyde and superoxide dismutase. Olympic. 2009. Year 18. No. 3. Pages: 147-137.
-Naziroğlu M, Butterworth PJ. Protective effects of moderate exercise with dietary vitamin C and E on blood antioxidative defense mechanism in rats with streptozotocin-induced diabetes. Canadian journal of applied physiology. 2005; 30(2):172-85.
-Riso P, Visioli F, Gardana C, Grande S, Brusamolino A, Galvano F, Galvano G, Porrini M. Effects of blood orange juice intake on antioxidant bioavailability and on different markers related to oxidative stress. Journal of Agricultural and Food Chemistry. 2005; 53(4):941-7.
- Abstract Viewed: 45 times