The effect of omega-3 fatty acid supplementation on Nitric oxide (NO) serum levels and anthropometric indices in men with cardiovascular disease referred to Tehran Heart Center: A randomized, double-blind, placebo-controlled trial
Researcher Bulletin of Medical Sciences,
Vol. 29 No. 1 (2024),
4 January 2025
,
Page e13
Abstract
Objective: Cardiovascular diseases (CVDs) are among the most common non-communicable diseases. They are the leading cause of death worldwide. Omega-3 unsaturated fatty acids are not produced in the human body, so they are essential for humans and are vital for the regulation of human performance. The aim of this study was to evaluate the effect of omega-3 fatty acid supplementation on Nitric oxide (NO) serum levels and anthropometric indices in men with cardiovascular disease.
Materials and Methods: In this double-blind study, 40 male Wistar rats were divided into 4 groups (N: 10). The groups were: Control group, supplement group, exercise group, and exercise + supplement group. At the end, blood was drawn from all the animals, then the serum lipid profile, were measured by ELISA method. They were analyzed with SPSS and a significance level of <0.05.
Results: No statistically significant difference was observed in any of the anthropometric indicators and blood pressure levels (systolic and diastolic) before and after the intervention, as well as within the groups. Also, no significant difference was observed in the serum level of NO before and after the start of the study in both groups, and within each group before and after the intervention, there was no statistically significant difference (P>0.05).
Conclusion: The findings of the present study showed that 10 weeks of supplementing with omega-3 supplementation at a dose of 4 grams per day, didn’t cause any significant changes in serum levels of NO
- Omega-3 fatty acid; Nitric oxide (NO);Anthropometric indices; Cardiovascular disease
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References
Williams RA. Cardiovascular disease in African American women: a health care disparities issue. Journal of the National Medical Association.40-536:(6)101;2009.
Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. 1993.
Samaneh Ansari, Mahmoud Djalali, Niyaz Mohammadzadeh Honarvar, Maryam Mazaherioun, Mahnaz Zarei, Fahimeh Agh, Zahra Gholampour, and Mohammad Hassan Javanbakht. The Effect of n-3 Polyunsaturated Fatty Acids Supplementation on Serum Irisin in Patients with Type 2 Diabetes: A Randomized, Double-Blind, Placebo-Controlled Trial. Int J Endocrinol Metab. 2017 Jan; 15(1): e40614.
Institute of Medicine NAoS. Dietary reference intakes: energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids. Washington, DC: The National Academies Press. 2002.
Lavie CJ, Milani RV, Mehra MR, Ventura HO. Omega-3 polyunsaturated fatty acids and cardiovascular diseases. Journal of the American College of Cardiology.94-585:(7)54;2009.
Saravanan P, Davidson NC, Schmidt EB, Calder PC. Cardiovascular effects of marine omega-3 fatty acids. The Lancet. 2010;376(9740):540-50.
Geleijnse JM, Giltay EJ, Grobbee DE, Donders AR, Kok FJ. Blood pressure response to fish oil supplementation: metaregression analysis of randomized trials. Journal of hypertension. 2002;20(8):1493-9.
Connor WE. Importance of n− 3 fatty acids in health and disease. The American journal of clinical nutrition. 2000;71(1):171S-5S.
Loscalzo J. Oxidative stress in endothelial cell dysfunction and thrombosis. Pathophysiology of haemostasis and thrombosis. 2002;32(5-6):359-60.
Dauchet L, Amouyel P, Hercberg S, Dallongeville J. Fruit and vegetable consumption and risk of coronary heart disease: a meta-analysis of cohort studies. The Journal of nutrition. 2006;136(10):2588-93.
McQuillan BM, Hung J, Beilby JP, Nidorf M, Thompson PL. Antioxidant vitamins and the risk of carotid atherosclerosis: The perth carotid ultrasound disease assessment study (CUDAS). Journal of the American College of Cardiology. 2001;38(7):1788-94.
Steinberg D PS, Carew TE, et al. Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. N Engl J Med. 1989;320:915–24.
Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical biochemistry. 2004;37(2):112-9.
Harma M, Harma M, Erel O. Measurement of the total antioxidant response in preeclampsia with a novel automated method. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2005;118(1):47-51.
Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical biochemistry. 2004;37(4):277-85.
Erel O. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry. 2005;38(12):1103-11.
Sümeyra Şahin Bayram and Gül Kızıltan. The Role of Omega- 3 Polyunsaturated Fatty Acids in Diabetes Mellitus Management: A Narrative Review. Curr Nutr Rep. 2024 Jul 20;13(3):527–551. doi: 10.1007/s13668-024-00561-9.
Veigh et al. Dietary fish oil augments nitric oxide production or release in patients with type 2 (non-insulin-dependent) diabetes mellitus. Diabetologia. 193; 36(1);33-8
Frank Thielecke and Andrew Blannin. Omega-3 Fatty Acids for Sport Performance—Are They Equally Beneficial for Athletes and Amateurs? A Narrative Review. Nutrients 2020, 12, 3712; doi:10.3390/nu12123712.
Hajianfar et al. The effect of omega-3 on the serum visfatin concentration in patients with type 2 diabetes. J Res Med Sci. 2011;16(4):490-495.
Kratz et al. Dietary n-3 polyunsaturated fatty acids and energy balance in overweight or moderately obese men and women; a randomized controlled trial. Nutr Metab. 2009;6;24.
Cabo et al. Omega-3 fatty acids and blood pressure. British journal of nutrition (2012), 107, 195-200.
Kabir M, Skurnik G, Naour N, Pechtner V, Meugnier E, Rome S, et al. Treatment for 2 mo with n− 3 polyunsaturated fatty acids reduces adiposity and some atherogenic factors but does not improve insulin sensitivity in women with type 2 diabetes: a randomized controlled study. The American journal of clinical nutrition. 2007;86(6):1670-9.
Dawczynski C, Martin L, Wagner A, Jahreis G. n− 3 LC-PUFA-enriched dairy products are able to reduce cardiovascular risk factors: A double-blind, cross-over study. Clinical nutrition. 2010;29(5):592-9.
Emami Naini et al. Effect of omega-3 fatty acids on blood pressure and serum lipids in continuous ambulatory peritonal dialysis patients. J Res Pharm Pract. 2015; 4(3):135-141.
Johansen O, Seljeflot I, Høstmark AT, Arnesen H. The effect of supplementation with omega-3 fatty acids on soluble markers of endothelial function in patients with coronary heart disease. Arteriosclerosis, thrombosis, and vascular biology. 1999;19(7):1681-6.
Donnelly SM, Ali MA, Churchill DN. Effect of n 3 fatty acids from fish oil on hemostasis, blood pressure, and lipid profile of dialysis patients. J Am Soc Nephrol. 1992;2:1634 9..
Ebrahimi M, Ghayour-Mobarhan M, Rezaiean S, Hoseini M, Parizade S, Farhoudi F, et al. Omega-3 fatty acid supplements improve the cardiovascular risk profile of subjects with metabolic syndrome, including markers of inflammation and auto-immunity. Acta cardiologica. 2009;64(3):321-7.
Aliasghari F, Eftekhari MH, Babaei Beigi MA, Hasanzadeh J, Mazooji N. The effect of conjugated linoleic acids and omega-3 fatty acids supplementation on
some inflammatory and oxidative stress markers in atherosclerosis. Iranian Journal of Nutrition Sciences & Food Technology. Vol. 7, No. 4, Winter 2013.
López D, Möller M, Denicola A, Casós K, Rubbo H, Ruiz-Sanz JI, et al. Long-chain n-3 polyunsaturated fatty acid from fish oil modulates aortic nitric oxide and tocopherol status in the rat. British journal of nutrition. 2008;100(04):767-75.
Mutlu-Türkoðlu, Ü., et al., Increased plasma malondialdehyde and protein carbonyl levels and lymphocyte DNA damage in patients with angiographically defined coronary artery disease. Clinical biochemistry, 2005. 38(12): p. 1059-1065.
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