Deoxyguanosine Factors and Protein Carbonyl Groups as Markers ofOxidative Stress in the Fasting Individuals in Tehran
Abstract
For downloading the full-text of this article please click here.
Background and Objectives: Oxidative stress represents an imbalance between the production of reactive oxygen species and a biological system's ability to readily detoxify the reactive intermediates or to repair the resulting damage. Calorie restriction canreduce the damages caused by oxidative stress.
Materials and Methods: The subjects, who were healthy males and females able to fast for a month, were randomly selectedfrom the staff of Hippocrates and Imam Khomeini hospitals. Samples of their serum and urine were collected three times, i.e. 4 days before fasting as control, on the 14th day of fasting, and on the 29th day of fasting. Plasma malondialdehyde levels were measured using HPLC technique, while the levels of protein carbonyl groups in plasma, and the levels of 8-iso prostaglandin F2α and 8-hydroxy-2'-deoxyguanosine in urine were measured by ELISA technique. The data were analyzed using SPSS, t test paired samples, Independentttest the level of statistical significance was considered to be p <0.05.
Results: Totally, 60 subjects participated in the study, where seven participants were male (11%) and 53 subjects (89%) were female. Mean and SD of the age of the participants were 37±10 years. Concentrations of plasmamalondialdehyde and 8-iso prostaglandin F2α in urine showed a significant decrease during fasting in Ramadan in all subjects compared with the control samples; moreover, their levels in the third sampling round were significantly lower than that in the second one (p < 0.01).
Conclusion: Since plasma levels of MDA and urin levels of 8-isoPGF significantly were decreased during fasting in Ramadan.It seems that fasting a month decreased oxidative stress. we hope that with more study in future ,we can use Ramadan fasting as a natural way to protect human against different disease which oxidative stress is involved.
Keywords: Fasting, Malondialdehyde, 8-iso prostaglandin F2α, 8-hydroxy-2-deoxyguanosine, Protein carbonyl
For downloading the full-text of this article please click here.
References
-de Diego-Otero Y, Romero-Zerbo Y, Bekay R, Decara J, Sanchez L, Rodriguez-de Fonseca F,et al. Alpha-tocopherol protects against oxidative stress in the fragile X knockout mouse. Neuro Psycho Pharmacology 2009; 34 (4): 1011–26
-B.M, M. MP.Aging .Res Rev2006; 5: 332-353.
-.DuanW ,M. MP. Aging. Neuro J Sci Res1999;57: 195-206
-W RL. Endogenus gene toxic ayed rats and procecess as abasis of spontaneous carcinoyenesis . Mutant Res1990 ; 238 : 287-295.
- Hart RW, Dixit R . Adaptive role of caloric intake on the degenerative disease. Toxicology Sci 1999 ; 52:3-12
-Cadenas E. Biochemistry of oxygen toxicity. Ann Rev Biochem1989;58:39-110
- Skrhga J , kuhesove M. short-term very low calory diet reduces oxidative stress in obese type 2 diabetic patient .Physiol 2000 ; 5u :33-39
-jiang T, Scott E. Caloric restriction modulation experssion of sterol regulatory element binding proteins,lipid accumulation and age related renaldisease. AM J Soc Nephro 2005 ; l16.
- Holee K, Bartsch H. Effect of short term fasting on urinary excretion of primary lipid peroxidant in products and markers of oxidative DNA damage in healthy women. Institute for Enviromental Medicin.SNUMRC Seoul 2006;1-17.
- Renke J, Popadiuk S, Korzon M, Bugajczyk B ,Wozniak M . Protein carbonyl groups content as a useful clinical marker of antioxidant barrier impairment in plasma of children with juvenile chronic arthritis. Free Radic Biol Med 2000; 292:101–104. 11.Tavernarakis N, Driscoll M. Caloric restriction and lifespan: a role for protein turnover Mech Ageing Dev 2002;123(2-3):215-29.
- Ugochukwu NH, Bagayoko ND, Antwi ME. The effects of dietary caloric restriction on antioxidant statue and lipid peroxidation in mild and severe streptozotocin-induced diabetic rats. Clin Chim Acta2004; 348:121–12
- Thompson HJ, Heimendinger J , Haegele A, Sedlacek SM,Gillette C, O'Neill C,et al. Effect of increased vegetable and fruit consumption on markers of oxidative cellular damage Carcinogenesis 1999; 20: 2261–226
- Anson R M,GuoZ. Intermittent fasting dissociated beneficaleffects of dietary restriction on glucose metabolism and neuronal resistance to injury from caloric intake. PARS 2003;100(10): 6216-6220
- Bevilacqua L, RamseyJJ, Hagopian K, Weindruch R, Harper ME. Effect of short- and medium caloric restriction on muscle mitochondrial proton leak and reactive oxygen species production. Am J Physiol. Endocrinol Metab 2004 ; 286: E852–E861
- Ümit Mutlu-Türko lu, Ebru lhan, Serdar Öztezcan, Alev Kuru, Gülçin Aykaç-Toker ,Müjdat Uysal,et al. Age-related increases in plasma malondialdehyde and protein carbonyl levels and lymphocyte DNA damage in elderly subjects. April 2003
- Meydani M , Band M , Epstein S, Das S , Roberts S. The effect of caloric restriction and glycemic load on measures of oxidative stress and antioxidants in humans. AGE 2006; 28(1):53.
- Lambert AJ, Merry BJ. Effect of caloric restriction on mitochondrial reactive oxygen species production and bioenergetics, reversal by insulin." AM J Physiol 2004 ;268: 71-79.
- Bevilacqus L, Jonj R. Effects of short and medium caloric restriction on muscle mitochondria proton leak and ROS production. AM J Physiol Endocrin Metab2004; 268: E852-E86I.
Published
Issue
Section
License
Under Bethesda Statement on Open Access Publishing (June 20, 2003), cience-research Journal of Pizhūhish dar dīn va salāmat are fully compliant with open access mandates, by publishing its articles under Creative Commons Attribution 4.0 International License (CC BY 4.0). Authors retain copyright of their work and can deposit their publication in any repository. This means that articles can be freely reused by the author and others as long as the article is properly cited. Published articles in Shahid Beheshti University of Medical Sciences Journals can be deposited immediately into an online repository without any cost. This articles can be, printed, archived in a collection, included in course-packs, and distributed without restrictions. Please read the full Creative Commons License for further information.
Creative Commons Attribution 4.0 International License.
