Journal of Ophthalmic and Optometric Sciences

Vol. 8 No. 1 (2024)

Comparing the effect of Mechanical and Tetracaine-Assisted Epithelial Debridement on Central Corneal Endothelial Cells in PRK

Journal of Ophthalmic and Optometric Sciences, Vol. 8 No. 1 (2024), 1 January 2024 , Page 1-9

Abstract

Purpose: To compare the effects on central corneal endothelial cells measured by confocal microscopy after photorefractive keratectomy (PRK) with mitomycin C (MMC) using mechanical and tetracaine-assisted methods.
Patients and Methods: In this prospective, randomized, comparative study, 44 patients (88 eyes) with myopia of up to -6.00 diopters were enrolled. Each patient's right eye was randomly assigned to either the tetracaine-assisted or the mechanical debridement group, with the left eye assigned to the other method. Preoperative and 3-month postoperative assessments were conducted using confocal microscopy. These assessments measured epithelial thickness, the number of keratocytes in the anterior, mid, and posterior stroma, and the characteristics of central corneal endothelial cells. These characteristics included cell density, mean cell area, polymegathism, and hexagonality.
Results: No statistically significant differences were observed between the two groups considering the mean epithelial thickness at the 3-month follow-up. Additionally, there were no statistically significant differences in the central corneal endothelial cells characteristics including the mean cell density, mean cell area, mean hexagonality, or mean polymegathism. The density of mid and posterior stromal keratocytes remained unchanged compared to baseline in both groups. However, a significant decrease in anterior stromal keratocyte density was observed in both groups three months after surgery, with no significant difference between the two debridement methods.
Conclusion: The effects of PRK with MMC using mechanical or tetracaine-assisted epithelial debridement methods on central corneal endothelial cells are similar. Additionally, both techniques lead to comparable reductions in stromal keratocyte density in anterior stromal layer.

Keywords:
  • Photorefractive Keratectomy
  • Cornea
  • Endothelial Cell Loss
  • Mitomycin C
  • Epithelium
  • Debridement

How to Cite

Nassiri, N., Shahriari , M., Porkar Rezaeyeh , A., Kavousnezhad , S., & Sheibani , K. (2024). Comparing the effect of Mechanical and Tetracaine-Assisted Epithelial Debridement on Central Corneal Endothelial Cells in PRK. Journal of Ophthalmic and Optometric Sciences, 8(1), 1–9. Retrieved from https://journals.sbmu.ac.ir/basir/article/view/47499

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