Impact of Perioperative Management on Ocular Microbiota Composition and Diversity: A Study of Intravitreal Injection Patients with 16S rRNA Sequencing
Journal of Ophthalmic and Optometric Sciences,
Vol. 5 No. 4 (2021),
1 May 2023
,
Page 14-29
https://doi.org/10.22037/joos.v5i4.40750
Abstract
Background: The ocular microbiota, which includes both commensal and pathogenic microorganisms, is constantly exposed to the ocular surface.
Material and Methods: In this study, two groups of patients were analyzed. Group A included 19 individuals who had not received intravitreal injections or undergone perioperative management. Group B, on the other hand, consisted of 22 patients who had received one, two, or more two treatments. The microbial samples collected from the ocular surface of these patients were subjected to 16S rRNA sequencing using the HiSeq 2500 platform. Further analysis of the alpha/beta diversity and clustering of operating taxonomic units (OTUs) was carried out.
Results: Our results show a significant difference in beta diversity was observed between group A (15 patients without intravitreal injections or perioperative management) and group B (patients with at least one, twice, or more than twice treatment) with a P value of 0.014. It was found that both the composition and relative abundance of cells were impacted by perioperative management in the lead-up to intravitreal injection. Additionally, a greater diversity of Gram-negative bacteria was observed and the most significant groups of microbiotas were found to be phyla and genera.
Conclusion: In conclusion, our study found that perioperative management has a significant impact on the ocular microbiota, altering its composition and disrupting its balance. Therefore, it is important for clinicians to carefully consider perioperative management prior to administering intravitreal injections.
- Intravitreal Injection
- Antimicrobial Resistance
- Ocular Surface Microbiota
- Perioperative Management
How to Cite
References
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