Transcriptomic Analysis of Human Retina Reveals Molecular Mechanisms Underlying Diabetic Retinopathy in Sexually Divergent Manner
Journal of Ophthalmic and Optometric Sciences,
Vol. 4 No. 2 (2020),
10 April 2020
,
Page 15-26
https://doi.org/10.22037/joos.v4i2.37343
Abstract
Today, retinopathy is one of the major causes of vision loss. With the increasing prevalence of obesity, diabetes, blood fat, and hypertension, the number of patients with retinopathy is increasing. Gender is an important factor in a variety of retinal diseases but has rarely been studied in clinical and biological studies. The current understanding of the effect of gender on molecular changes and pathways involved in the onset and progression of diabetic retinopathy (DR) is limited.
The aim of this study is to investigate the differences in Diabetic patients’ retinal gene expression between the two sexes. Through RNAseq analysis, mRNA expression profiles were analyzed from 40 post-mortem samples from 20 patients with diabetic macular edema (DME) stage of DR. 29 of samples were female and 11 were male. So our groups were control-female, DME-female, control-male and, DME-male. Human retinal single-cell RNA‑Sequencing data revealed 245 differently expressed genes in female-DME and male-DME patients.
The results of enrichment analysis show that most up-regulated genes take part in pathways involved in Osteoclast-associated receptor (OSCAR) binds collagen and Surfactant protein D (SP-D), apoptosis, and tyrosine metabolism molecular functions.
In this study, we detected a significant association between tyrosine metabolism and diabetic retinopathy in the DME stage. The increased DR risk was observed only in female patients with the abnormality of low tyrosine metabolism. Furthermore, we found a significant interaction between DR and the coexistence of low tyrosine metabolism. Suggesting that control of tyrosine metabolism might confer great risk reduction for DR in female patients.
- Diabetic Retinopathy
- Transcriptomic Analysis
- Retina
- Differential Gene Expression
How to Cite
References
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