ISSN: 2538-5224
Continuous Volume
Vol. 8 No. 1 (2022)
Protective Effect of Retinoic acid preconditioning on H2O2-induced Apoptosis in Hair Cell-like Cells
Journal of Otorhinolaryngology and Facial Plastic Surgery,
Vol. 8 No. 1 (2022),
,
Page 1-7
https://doi.org/10.22037/orlfps.v8i1.37751
Abstract
Background: Several researches have proven that high levels of noise can induce oxidative stress and increase free radical's formation, particularly hydrogen peroxide (H2O2) and nitric oxide (NO) production. Retinoids are polyisoprenoid lipids derived from vitamin A or retinol. These molecules are essential regulatory elements in cell processes. All-trans retinoic acid (ATRA) plays a vital function in cellular growth, apoptosis, reproduction, cell differentiation, and immune feature by binding to its nuclear receptors.
Aim: In this study, we studied the protective effect of ATRA on H2O2-induced damage to bone marrow mesenchymal stem cell (BMSCs)-derived hair cells in culture.
Methods: Expression of MATH1 and SOX2 genes were assayed by immunocytochemistry (ICC). In order to evaluate the tolerance of ATRA-treated cells, after incubation of hair cells-like cells with ATRA, it was exposed to H2O2 as an oxidative stress model. Then, the apoptosis percentage of cultivated hair cell-like cells was evaluated by acridin-orange staining method.
Results: Our findings revealed that apoptotic cells were markedly diminished in the ATRA +H2O2 co-treated cells in comparison with the H2O2 only–treated group.
Conclusion: ATRA has the protective effect against oxidative stress damage in the cultivated hair cells- like cells by reducing the apoptosis.
Keywords:
- All-trans retinoic acid, H2O2, Apoptosis, Hair cell-like cells.
How to Cite
Niknazar, S., Changaee, N., Abbaszadeh, H.-A., Peyvandi, M., & Peyvandi, A. A. (2022). Protective Effect of Retinoic acid preconditioning on H2O2-induced Apoptosis in Hair Cell-like Cells . Journal of Otorhinolaryngology and Facial Plastic Surgery, 8(1), 1-7. https://doi.org/10.22037/orlfps.v8i1.37751
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13. Karmakar S, Banik NL, Ray SK. Combination of all‐trans retinoic acid and paclitaxel‐induced differentiation and apoptosis in human glioblastoma U87MG xenografts in nude mice. Cancer: Interdisciplinary International Journal of the American Cancer Society. 2008;112(3):596-607.
14. Ross AC, Chen Q, Ma Y. Vitamin A and retinoic acid in the regulation of B-cell development and antibody production. Vitamins & Hormones. 2011;86:103-26.
15. Daniali S, Nahavandi A, Madjd Z, Shahbazi A, Niknazar S, Shahbazzadeh D. Chronic Ritalin administration during adulthood increases serotonin pool in rat medial frontal cortex. Iranian Biomedical Journal. 2013;17(3):134.
16. Niknazar S, Abbaszadeh H-A, Peyvandi H, Rezaei O, Forooghirad H, Khoshsirat S, et al. Protective effect of [Pyr1]-apelin-13 on oxidative stress-induced apoptosis in hair cell-like cells derived from bone marrow mesenchymal stem cells. European journal of pharmacology. 2019;853:25-32.
17. Didenko VV, Ngo H, Baskin DS. Early necrotic DNA degradation: presence of blunt-ended DNA breaks, 3′ and 5′ overhangs in apoptosis, but only 5′ overhangs in early necrosis. The American journal of pathology. 2003;162(5):1571-8.
18. Bakhtiarzadeh F, Nahavandi A, Goudarzi M, Shirvalilou S, Rakhshan K, Niknazar S. Axonal transport proteins and depressive like behavior, following Chronic Unpredictable Mild Stress in male rat. Physiology & behavior. 2018;194:9-14.
19. Redza-Dutordoir M, Averill-Bates DA. Activation of apoptosis signalling pathways by reactive oxygen species. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. 2016;1863(12):2977-92.
20. Chen M, Huang J. Retinoic acid induces differentiation of cochlear neural progenitor cells into hair cells. Brazilian Journal of Otorhinolaryngology. 2021.
21. Katsuki H, Kurimoto E, Takemori S, Kurauchi Y, Hisatsune A, Isohama Y, et al. Retinoic acid receptor stimulation protects midbrain dopaminergic neurons from inflammatory degeneration via BDNF‐mediated signaling. Journal of neurochemistry. 2009;110(2):707-18.
22. Ahlemeyer B, Krieglstein J. Inhibition of glutathione depletion by retinoic acid and tocopherol protects cultured neurons from staurosporine-induced oxidative stress and apoptosis. Neurochemistry international. 2000;36(1):1-5.
23. Ahlemeyer B, Bauerbach E, Plath M, Steuber M, Heers C, Tegtmeier F, et al. Retinoic acid reduces apoptosis and oxidative stress by preservation of SOD protein level. Free Radical Biology and Medicine. 2001;30(10):1067-77.
24. Chatterjee A, Chatterji U. All‐trans retinoic acid ameliorates arsenic‐induced oxidative stress and apoptosis in the rat uterus by modulating MAPK signaling proteins. Journal of cellular biochemistry. 2017;118(11):3796-809.
25. Sakamoto K, Hiraiwa M, Saito M, Nakahara T, Sato Y, Nagao T, et al. Protective effect of all-trans retinoic acid on NMDA-induced neuronal cell death in rat retina. European journal of pharmacology. 2010;635(1-3):56-61.
26. Thin TH, Li L, Chung TK, Sun H, Taneja R. Stra13 is induced by genotoxic stress and regulates ionizing‐radiation‐induced apoptosis. EMBO reports. 2007;8(4):401-7.
27. Li J, Orr B, White K, Belogortseva N, Niles R, Boskovic G, et al. Chmp 1A is a mediator of the anti-proliferative effects of all-trans retinoic acid in human pancreatic cancer cells. Molecular cancer. 2009;8(1):1-13.
28. Dong W, Zhang Y. Retinoic acid receptor α expression exerts an anti apoptosis effect on PC12 cells following oxygen glucose deprivation. Experimental and therapeutic medicine. 2018;16(4):3525-33.
2. Seidman MD, Khan MJ, Tang WX, Quirk WS. Influence of lecithin on mitochondrial DNA and age-related hearing loss. Otolaryngology—Head and Neck Surgery. 2002;127(3):138-44.
3. Study BSC, Health NIo. Information about Hearing, Communication, and Understanding. NIH Curriculum Supplement Series [Internet]: National Institutes of Health (US); 2007.
4. Fredelius L, Wersäll J. Hair cell damage after continuous and interrupted pure tone overstimulation: a scanning electron microscopic study in the guinea pig. Hearing research. 1992;62(2):194-8.
5. Hill K, Yuan H, Wang X, Sha S-H. Noise-induced loss of hair cells and cochlear synaptopathy are mediated by the activation of AMPK. Journal of Neuroscience. 2016;36(28):7497-510.
6. Cho Y, Gong T-WL, Kanicki A, Altschuler RA, Lomax MI. Noise overstimulation induces immediate early genes in the rat cochlea. Molecular Brain Research. 2004;130(1-2):134-48.
7. Chung JW, Kang HH, Shin JE, Kim JU. Accumulation of hypoxia-inducible factor-1α in mouse inner ear by noise stimulation. Neuroreport. 2004;15(15):2353-6.
8. Kinouchi H, Epstein CJ, Mizui T, Carlson E, Chen SF, Chan PH. Attenuation of focal cerebral ischemic injury in transgenic mice overexpressing CuZn superoxide dismutase. Proceedings of the National Academy of Sciences. 1991;88(24):11158-62.
9. Shinozaki Y, Sato Y, Koizumi S, Ohno Y, Nagao T, Inoue K. Retinoic acids acting through retinoid receptors protect hippocampal neurons from oxygen-glucose deprivation-mediated cell death by inhibition of c-jun-N-terminal kinase and p38 mitogen-activated protein kinase. Neuroscience. 2007;147(1):153-63.
10. Horschitz S, Matthäus F, Groß A, Rosner J, Galach M, Greffrath W, et al. Impact of preconditioning with retinoic acid during early development on morphological and functional characteristics of human induced pluripotent stem cell-derived neurons. Stem cell research. 2015;15(1):30-41.
11. Chen Q, Ross AC. Retinoic acid regulates cell cycle progression and cell differentiation in human monocytic THP-1 cells. Experimental cell research. 2004;297(1):68-81.
12. Bastien J, Rochette-Egly C. Nuclear retinoid receptors and the transcription of retinoid-target genes. Gene. 2004;328:1-16.
13. Karmakar S, Banik NL, Ray SK. Combination of all‐trans retinoic acid and paclitaxel‐induced differentiation and apoptosis in human glioblastoma U87MG xenografts in nude mice. Cancer: Interdisciplinary International Journal of the American Cancer Society. 2008;112(3):596-607.
14. Ross AC, Chen Q, Ma Y. Vitamin A and retinoic acid in the regulation of B-cell development and antibody production. Vitamins & Hormones. 2011;86:103-26.
15. Daniali S, Nahavandi A, Madjd Z, Shahbazi A, Niknazar S, Shahbazzadeh D. Chronic Ritalin administration during adulthood increases serotonin pool in rat medial frontal cortex. Iranian Biomedical Journal. 2013;17(3):134.
16. Niknazar S, Abbaszadeh H-A, Peyvandi H, Rezaei O, Forooghirad H, Khoshsirat S, et al. Protective effect of [Pyr1]-apelin-13 on oxidative stress-induced apoptosis in hair cell-like cells derived from bone marrow mesenchymal stem cells. European journal of pharmacology. 2019;853:25-32.
17. Didenko VV, Ngo H, Baskin DS. Early necrotic DNA degradation: presence of blunt-ended DNA breaks, 3′ and 5′ overhangs in apoptosis, but only 5′ overhangs in early necrosis. The American journal of pathology. 2003;162(5):1571-8.
18. Bakhtiarzadeh F, Nahavandi A, Goudarzi M, Shirvalilou S, Rakhshan K, Niknazar S. Axonal transport proteins and depressive like behavior, following Chronic Unpredictable Mild Stress in male rat. Physiology & behavior. 2018;194:9-14.
19. Redza-Dutordoir M, Averill-Bates DA. Activation of apoptosis signalling pathways by reactive oxygen species. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. 2016;1863(12):2977-92.
20. Chen M, Huang J. Retinoic acid induces differentiation of cochlear neural progenitor cells into hair cells. Brazilian Journal of Otorhinolaryngology. 2021.
21. Katsuki H, Kurimoto E, Takemori S, Kurauchi Y, Hisatsune A, Isohama Y, et al. Retinoic acid receptor stimulation protects midbrain dopaminergic neurons from inflammatory degeneration via BDNF‐mediated signaling. Journal of neurochemistry. 2009;110(2):707-18.
22. Ahlemeyer B, Krieglstein J. Inhibition of glutathione depletion by retinoic acid and tocopherol protects cultured neurons from staurosporine-induced oxidative stress and apoptosis. Neurochemistry international. 2000;36(1):1-5.
23. Ahlemeyer B, Bauerbach E, Plath M, Steuber M, Heers C, Tegtmeier F, et al. Retinoic acid reduces apoptosis and oxidative stress by preservation of SOD protein level. Free Radical Biology and Medicine. 2001;30(10):1067-77.
24. Chatterjee A, Chatterji U. All‐trans retinoic acid ameliorates arsenic‐induced oxidative stress and apoptosis in the rat uterus by modulating MAPK signaling proteins. Journal of cellular biochemistry. 2017;118(11):3796-809.
25. Sakamoto K, Hiraiwa M, Saito M, Nakahara T, Sato Y, Nagao T, et al. Protective effect of all-trans retinoic acid on NMDA-induced neuronal cell death in rat retina. European journal of pharmacology. 2010;635(1-3):56-61.
26. Thin TH, Li L, Chung TK, Sun H, Taneja R. Stra13 is induced by genotoxic stress and regulates ionizing‐radiation‐induced apoptosis. EMBO reports. 2007;8(4):401-7.
27. Li J, Orr B, White K, Belogortseva N, Niles R, Boskovic G, et al. Chmp 1A is a mediator of the anti-proliferative effects of all-trans retinoic acid in human pancreatic cancer cells. Molecular cancer. 2009;8(1):1-13.
28. Dong W, Zhang Y. Retinoic acid receptor α expression exerts an anti apoptosis effect on PC12 cells following oxygen glucose deprivation. Experimental and therapeutic medicine. 2018;16(4):3525-33.
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