The antibacterial effect of methanolic fraction of Nepeta depauperataon against Pseudomonas aeruginosa isolates from burn wound infections
Background: In this study, the antibacterial effect of the methanolic fraction of Nepeta depauperata against 50 isolates of Pseudomonas aeruginosa from burn wound infections of patients who referred to Shahid Motahari hospital of Tehran in 2014 was evaluated.
Materials and Methods: All bacterial isolates were confirmed by standard bacteriologic methods. Their resistant to common antibiotics were evaluated by disk diffusion method based on CLSI 2014. The Nepeta depauperata aerial parts were collected from Hormozgan Province and identified. Methanolic extract was prepared by maceration method using percolator apparatus and concentrated by rotary evaporator. The antibacterial activity of methanolic extract were determined by two methods; cup plate diffusion agar for determination the zone diameter of inhibition and microdilution broth for minimum inhibitory concentration (MIC) and further minimum bacteriocidal concentration (MBC). Statistical analysis was done by SPSS software version 20.
Results: The percentage of resistance and susceptibility against nine different kinds of common antibiotic disk showed 83% resistance on average as evaluated by agar disk diffusion (Kirby–Bauer antibiotic test). Also, the mean of inhibition zone diameters has been measured in concentrations of 1000, 500, 250 mg/ml as follow: 12.58, 11.3 and 9.44 mm, respectively by cup plate and the amount of 87.93 and 104.78 mg/ml for MIC and MBC were determined, respectively, using the broth microdilution method. Statistic analysis was done with SPSS verssion19 software.
Conclusion: According to the satisfying results of the antibacterial effect of the testing methanolic extract against clinical isolates of P. aeruginosa isolates further in vitro and in vivo studies are recommended.
Vahdani M, Azimi L, Asghari B, Bazmi F, Rastegar Lari A. Phenotypic screening of extended-spectrum ß-lactamase and metallo-ß-lactamase in multidrug-resistant Pseudomonas aeruginosa from infected burns. Ann Burns Fire Disasters. 2012;25:78-81.
Formisano C, Rigano D, Senatore F. Chemical constituents and biological ctivities of Nepeta species. Chem Biodivers. 2011;8:1783- 818.
Jamzad Z, Grayer RJ, Kite GC, Simmonds MSJ, Ingrouille M, Jalili A. Leaf surface flavonoids in Iranian species of Nepeta (Lamiaceae) and some related genera. Biochem Syst Ecol. 2003;31:587-600.
Asgarpanah J, Sarabian S, Ziarati P. Essential oil of Nepeta genus (Lamiaceae) from Iran: a review. J Essent Oil Res. 2014;26(1):1-12.
Mozaffarian V. A Dictionary of Iranian Plants Names. Tehran: Farhang Moaser Press; 1995.
Mehrabani M, Asadipour A, Saber-Amoli S. Chemical constituents of the essential oil of Nepetadepauperata from Iran. Daru J Pharm Sci. 2004;12:98-100.
Kariminejad S, Abdnikfarjam M, Hosseini Doust R, Hakemi-Vala M, Asgarpanah J. Antibacterial and antifungal activities of the endemic species Nepeta depauperata Benth from Iran. Ethno-Pharmaceutical products. 2014:9-13.
Shahcheraghi F, Abbasalipour M, Feizabadi M, et al. Isolation and genetic characterization of metallo-beta-lactamase and carbapenamase producing strains of Acinetobacter baumannii from patients at Tehran hospitals. Iran J Microbiol. 2011;3:68-74.
Fallah F, Borhan RS, Hashemi A: Detection of bla(IMP) and bla(VIM) metallo-beta-lactamases genes among Pseudomonas aeruginosa strains. Int J Burns Trauma. 2013;3:122-4.
Wayne PC. Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial susceptibility testing. Twenty-second informational supplement. Document M100-S22; Pennynsylvania, USA; 2012.
Fazly-Bazzaz BS, Khajehkaramadin M, Shokooheizadeh HR. Antibacterial activity of Rheum ribes extract obtained from various plant parts against clinical isolates of Gram-negative pathogens. Iran J Pharm Res. 2005;2:87-91.
Mehregan H, Mojab F, Pakdaman SH, Poursaeed M. Antibacterial activity of Thymus pubescencemethanolic extract. Iran J Pharm Res. 2008;7(4):291-5.
Irobi ON, Darambolo SO. Antifungal activity of crude extracts of Mitracarpusvillosus (Rubiaceae). J Ethnopharmacol. 1993;40:137-40.
Vaijayantimala J, Rajendra-Prasad N, Pugalendi KV. Antifungal activity of oils. Ind J Microbiol. 2001;41:325-8.
Sonboli A, Gholipour A, Yousefzadi M, Mojarrad M. Antibacterial activity and composition of the essential oil of Nepetamenthoides from Iran. Nat Prod Commun. 2009;4(2):283-6.
Gautam SS, Navneet S, Kumar S, Prabhat A. Screening of antibacteial activity of NepetaciliarisBenth. against respiratory tract pathogens. Kathmandu Uni J SciEngeen Technol. 2012;8(1):100-3.
Nezhadali A, Masrornia M, Bari H, Akbarpour M, Joharchi MH, Nakhaei-Moghadam M.Antibacterial activity and composition of essential oil of Nepetapungens Benth from Iran. J Essent. Oil Bear Pl. 2011;14(2):241-4.
Grbic ML, Stupar M, Vukojevic J, Sokovic M, Misic D, Grubisic D, Ristic M. Antifungal activity of Nepeta rtanjensis essential oil. J Serb Chem Soc. 2008;73(10):961-5.
Sexena J, Mathela CS. Antifungal activity of new compounds from Nepetaleucophylla and Nepetaclarkei. Appl Environ Microbiol. 1996;62(2):702-4.
Kordali S, Usanmaz A, Cakir A, Cavusoðlu A, Ercisli S. In Vitro antifungal effect of essential oils from Nepeta meyeri Benth. Egyp J Biol Pest Cont. 2013;9(3):404-18.