Translational Medicine Student Research in Translational Medicine journal SRTM SMSJ School of medicine students' journal smsjournal biomedical studies basic medical sciences clinical research public health medical education Original article Narrative review Systematic Reviews and Meta Analysis Short communication Visual practice Photo Video Study protocol Hypothesis Case report Editorial

Vol. 6 (2024)

Original Article


Nanoliposomal Auraptene: A Comprehensive Study on Preparation, Characterization, Cytotoxicity, and Anti-Angiogenic Potential

Tara Emami, Shiva Ghafghazi, Roghaieh Tarasi, Mohammad Abbas Sheikholeslami, Fatemeh Kalhor, Seyed Ali Ziai

Student Research in Translational Medicine, Vol. 6 (2024), 24 January 2024,
https://doi.org/10.22037/srtm.v6.44191

Background: Nanoliposomes are spherical nano-sized capsules enclosed by lipid membranes, serving as a biocompatible vehicle to enhance the delivery of therapeutic agents. The objective of this research is to prepare and characterize nanoliposome-encapsulated auraptene and compare its cytotoxic and anti-angiogenic effects to non-liposomal auraptene.

Methods: Liposomal auraptene was formulated using DSPC/ DSPG/ Cholesterol (molar ratio of 4:1:2) in combination with two different molar ratios of auraptene (0.1 and 0.05). The entrapment efficiency was evaluated using High- Performance Liquid Chromatography (HPLC). Various parameters, including Dynamic Light Scattering (DLS), zeta potential, stability, and release kinetics, were investigated. Subsequently, both liposomal and non-liposomal auraptene, along with bare liposomes, were applied to the MDA-MB-231 cell line for duration of 72 hours at 37°C at varying concentrations. Cytotoxicity was assessed using the MTT assay. Additionally, the study examined the anti-angiogenic effects on the vessels in the chorioallantoic membrane of chick embryos.

Results: The entrapment efficiency of auraptene was found to be satisfactory at 50%. The liposome size ranged from 85 to 241 nm, with a Z-Average of 190.9 nm. The zeta potentials for all formulations were consistently around -55.7, and the Polydispersity Index (PDI) was less than 0.3 for all formulations. The release profile demonstrated approximately 80% drug release over a period of 130 hours. Notably, liposomal auraptene exhibited a significantly lower IC50 value (38.61 (95% Confidence Interval: 30.56 to 48.78)) compared to non-liposomal auraptene (50.36 (95% Confidence Interval: 43.58 to 58.19)) (p = 0.0240).

Conclusion: Moreover, the administration of 2.5 and 5 µM of liposomal auraptene led to a reduction in the vessels within the chorioallantoic membrane at the injection site when compared to the control group.

In summary, the use of biodegradable nanoliposomal carriers improved the solubility, release profile, and stability of auraptene while demonstrating anticancer and anti-angiogenic properties.

Open Versus Laparoscopic Surgery for Restoration of Incisional Hernia: A Case- Control Study

Nasser Malekpour Alamdari, Hamidreza Hasankhani, Yeganeh Farsi, Babak Sabet Diveshali, Barmak Gholizadeh

Student Research in Translational Medicine, Vol. 6 (2024), 24 January 2024,
https://doi.org/10.22037/srtm.v6.40024

Background and Aim: Incisional hernia is one of the most notable surgical complications that can be repaired either laparoscopically or by open surgery. This study aims to compare the surgery- related factors and surgical outcomes between these two groups.

Methods: This is a retrospective single center study, investigates the surgical outcomes of patients that have underwent either open or laparoscopic surgical repair in a tertiary hospital in Tehran, between 2019- 2020.

Results: 70 patients (35 in each group) enrolled the study. 71.42 % of patients were female and the mean age of total study sample was 53.12 ± 11.66 years. There were significant lower pain score, operation time, and hospitalization length in laparoscopic cohort (p< 0.05). There was no significant difference between rates of post- operative complications including seroma, hematoma, surgical site infection, and ileus (p> 0.05). Laparoscopic surgery significantly costs more than open surgery (p< 0.05). There was no case of recurrence within the 12-months after surgery.

Conclusion: In conclusion, while laparoscopic surgery costs more than open surgery, it is associated with significant decrease in pain score, operation time, and hospitalization length. There is no significant difference between post-operative complications including seroma, hematoma, and surgical site infection among two groups.

Hypothesis


Inhalation of Mesenchymal Stem Cell- Derived Small Extracellular Vesicles as a Possible Approach to Ameliorating Acute Lung Injury Caused by Cytokine Storm

Zahra Mirsanei, Fatemeh Jamshidi-Adegani, Fatemeh Ahangari, Sara Soufihasanabad, Sara Soudi, Saeid Vakilian, Sulaiman Al-Hashmi, Seyed Mahmoud Hashemi

Student Research in Translational Medicine, Vol. 6 (2024), 24 January 2024,
https://doi.org/10.22037/srtm.v6.44112

Lung is one of the vital organs that get severely affected by cytokine storm and sepsis, leading to the development of acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). Mesenchymal stem cell- derived small extracellular vesicles (MSC- derived sEVs) are one of the therapeutic approaches for ARDS/ ALI caused by sepsis. Apart from sEVs’ ability to carry medication, MSC- derived sEVs also possess anti- inflammatory, tissue repair, and regeneration properties. Targeted drug delivery has been a crucial area of concern in the medical field, specifically for treating lung diseases. To treat lung diseases locally, inhalation of drug products has been developed. Drug delivery by inhalation has emerged as an effective method for local administration of therapeutic agents, with numerous benefits including better efficacy at lower doses and decreased toxicity. Additionally, inhalation administration is a viable option for the systemic distribution of medications due to the lungs' considerable absorption surface and their ability to bypass initial metabolism. Therefore, our hypothesis proposes the inhalation of MSC- derived sEVs as a potential strategy for alleviating acute lung injury induced by sepsis-related cytokine storm. Following the isolation and characterization of these MSC- derived sEVs, they will be administered to an animal model of sepsis via a nebulizer, either in their pure form or loaded with drugs. Several approaches will be employed to evaluate lung functionality, including histological analysis and the measurement of inflammatory and regulatory cytokine levels to assess changes in both the humoral and cellular immune systems.

Study Protocol


Affinity Determination of Monoclonal Antibodies (mAbs) Using Enzyme- Linked Immunosorbent Assay (ELISA); A Protocol

Mansoure Mansouri, Mehdi Mohammadi, Fatemeh Montazer, Mahmood Jeddi-Tehrani, Mahdi Shabani

Student Research in Translational Medicine, Vol. 6 (2024), 24 January 2024,
https://doi.org/10.22037/srtm.v6.44442

Monoclonal antibodies (mAbs) have changed diagnostics and therapy due to their high specificity and affinity to the target antigens (Ags). Accurately measuring the affinity of mAbs is critical to understanding their binding properties. It represents the strength of binding between an antibody (Ab) and its target Ag and enables decision making in the development and optimization of these antibodies to improve their efficacy in diagnostics and therapy. Various methods such as the equilibrium dissociation constant, ELISA, surface plasmon resonance (SPR), and microarray-based platforms can be used to determine mAb affinity. The non-competitive ELISA is simple and available method for many laboratories, based on the law of mass action that compares the OD50 of three sigmoidal curves of serial Ab dilutions on plates coated with different Ag concentrations to determine the binding strength between a mAb and its Ag. This protocol provides a step-by-step guide to determining mAb affinity using modified ELISA and enables researchers to make informed decisions about the development and application of mAbs in their respective fields.