Differential diagnosis of tuberculosis, sarcoidosis, and anthracosis by CD8, CD3, and CD4 levels by flow-cytometry
School of Medicine Students' Journal,
Vol. 1 No. 1 (2019),
10 December 2019
Background: Determination of a desirable diagnostic tests is an issue of importance especially to differentiate between tuberculosis) TB(, sarcoidosis, and anthracosis. The purpose of the present study was to determine the differential diagnosis of tuberculosis, sarcoidosis and anthracosis with CD8, CD3, and CD4 by flow-cytometry.
Materials and Methods: In this descriptive cross-sectional comparative survey, 40 consecutive patients attending to Masih Daneshvari Hospital in Tehran were enrolled and CD4/CD8 ratio, CD8, CD3, and CD4 were determined by flow-cytometry and compared across patients with three diseases including tuberculosis, sarcoidosis, and anthracosis.
Results: The results demonstrated that CD4 was significantly higher in anthracosis cases (P<0.007) and the CD8 was significantly higher in patients with TB (P<0.008).
Conclusion: It was attained ultimately that CD4 and CD8 levels could be a desirable diagnostic markers for anthracosis and TB, respectively.
- Broncho alveolar lavage (BAL)
How to Cite
R. P. Baughman, A. S. Teirstein, M. A. Judson, M. D. Rossman, H. Yeager Jr, E. A. Bresnitz, et al.Clinical characteristics of patients in a case control study of sarcoidosis.American journal of respiratory and critical care medicine.2001;164(10):1885-89.
A. T. Society.Statement on sarcoidosis. Joint Statement of the American Thoracic Society (ATS), the European Respiratory Society (ERS) and the World Association of Sarcoidosis and Other Granulomatous Disorders (WASOG) adopted by the ATS Board of Directors and by the ERS Executive Committee.Am J Respir Crit Care Med.1999;160(2):736-55.
M. D. Rossman, J. H. Dauber and R. P. Daniele.Identification of activated T cells in sarcoidosis.American Review of Respiratory Disease.1978;117(4):713-20.
J. Müller-Quernheim, S. Pfeifer, J. Strausz and R. Ferlinz.Correlation of clinical and immunologic parameters of the inflammatory activity of pulmonary sarcoidosis.American Review of Respiratory Disease.1991;144(6):1322-29.
M. C. Iannuzzi, B. A. Rybicki and A. S. Teirstein.Medical progress.N Engl J Med.2007;357:2153-65.
R. P. Baughman, D. A. Culver and M. A. Judson.A concise review of pulmonary sarcoidosis.American journal of respiratory and critical care medicine.2011;183(5):573-81.
J. H. Dauber, M. Wagner, S. Brunsvold, I. L. Paradis, L. A. Ernst and A. Waggoner.Flow cytometric analysis of lymphocyte phenotypes in bronchoalveolar lavage fluid: comparison of a two-color technique with a standard immunoperoxidase assay.American journal of respiratory cell and molecular biology.1992;7:531-31.
L. Newman. Medical progress-Sarcoidosis (vol 336, pg 1224, 1997). MASS MEDICAL SOC 10 SHATTUCK, BOSTON, MA 02115; 1997. p. 139-39.
L. Welker, R. Jörres, U. Costabel and H. Magnussen.Predictive value of BAL cell differentials in the diagnosis of interstitial lung diseases.European respiratory journal.2004;24(6):1000-06.
M. Drent, P. Mulder, S. S. Wagenaar, H. Hoogsteden, H. van Velzen-Blad and J. van den Bosch.Differences in BAL fluid variables in interstitial lung diseases evaluated by discriminant analysis.European Respiratory Journal.1993;6(6):803-10.
S. Sharma and A. Mohan.Sarcoidosis: global scenario & Indian perspective.Indian Journal of Medical Research.2002;116:221.
J. J. Swigris, A. L. Olson, T. J. Huie, E. R. Fernandez-Perez, J. Solomon, D. Sprunger, et al.Sarcoidosis-related mortality in the United States from 1988 to 2007.American journal of respiratory and critical care medicine.2011;183(11):1524-30.
D. K. Parkinson and M. J. Grennan Jr.Acute‐onset sarcoidosis presenting as workplace‐related hyperreactive airway disease.American journal of industrial medicine.1986;9(3):243-46.
K. Amoli.Anthracotic airways disease: Report of 102 cases.2009.
P. Mulliez, M. Billon-Galland, E. Dansin, X. Janson and J. Plisson.Bronchial anthracosis and pulmonary mica overload.Revue des maladies respiratoires.2003;20(2 Pt 1):267-71.
P. Sonnenberg, J. Murray, R. Thomas, P. Godfrey-Faussett and S. Shearer.Risk factors for pulmonary disease due to culture-positive M. tuberculosis or nontuberculous mycobacteria in South African gold miners.European Respiratory Journal.2000;15(2):291-96.
L. A. Carson, L. Bland, L. Cusick, M. Favero, G. Bolan, A. Reingold, et al.Prevalence of nontuberculous mycobacteria in water supplies of hemodialysis centers.Appl Environ Microbiol.1988;54(12):3122-25.
A.-L. Roux, E. Catherinot, F. Ripoll, N. Soismier, E. Macheras, S. Ravilly, et al.Multicenter study of prevalence of nontuberculous mycobacteria in patients with cystic fibrosis in France.Journal of clinical microbiology.2009;47(12):4124-28.
K. S. Sachdeva, A. Kumar, P. Dewan, A. Kumar and S. Satyanarayana.New vision for Revised National Tuberculosis Control Programme (RNTCP): universal access-“reaching the un-reached”.The Indian journal of medical research.2012;135(5):690.
L. Nelson and C. Wells.Global epidemiology of childhood tuberculosis [Childhood TB].The International journal of Tuberculosis and lung Disease.2004;8(5):636-47.
D. Shingadia and V. Novelli.Diagnosis and treatment of tuberculosis in children.The Lancet infectious diseases.2003;3(10):624-32.
E. Mortaz, H. Gudarzi, P. Tabarsi, I. M Adcock, M. R. Masjedi, H. R. Jamaati, et al.Flow cytometry applications in the study of immunological lung disorders.Iranian Journal of Allergy, Asthma and Immunology.2015;14(1):12-18.
W. Barcelos, O. A. Martins‐Filho, T. M. P. D. Guimarães, M. H. P. Oliveira, S. Spíndola‐de‐Miranda, B. N. Carvalho, et al.Peripheral blood mononuclear cells immunophenotyping in pulmonary tuberculosis patients before and after treatment.Microbiology and immunology.2006;50(8):597-605.
N. J. Sweiss, R. Salloum, S. Ghandi, M.-L. Alegre, R. Sawaqed, M. Badaracco, et al.Significant CD4, CD8, and CD19 lymphopenia in peripheral blood of sarcoidosis patients correlates with severe disease manifestations.PLoS One.2010;5(2):e9088.
K. Kojima, K. Maruyama, T. Inaba, K. Nagata, T. Yasuhara, K. Yoneda, et al.The CD4/CD8 ratio in vitreous fluid is of high diagnostic value in sarcoidosis.Ophthalmology.2012;119(11):2386-92.
D. d. S. d. S. Rodrigues, E. A. S. d. Medeiros, L. Y. Weckx, W. Bonnez, R. Salomão and E. G. Kallas.Immunophenotypic characterization of peripheral T lymphocytes in Mycobacterium tuberculosis infection and disease.Clinical & Experimental Immunology.2002;128(1):149-54.
- Abstract Viewed: 1250 times
- PDF Downloaded: 22 times