A survey of Free-Living Amoebae (FLAs) in Nasal Mucosa of Healthy Individuals using Culture-based Method
Novelty in Biomedicine,
Vol. 9 No. 4 (2021),
12 November 2021
,
Page 204-206
https://doi.org/10.22037/nbm.v9i4.35486
Abstract
Free-living amoebas (FLAs) are facultative parasites, and some of them can be highly pathogenic for animals and humans. These protozoan are found in abundance in environmental resources such as freshwater and soil, and thus the possible colonization of such FLA is probable. Our previous studies showed that Acanthamoeba spp. can colonize nasal mucosa of immunosuppressed patients. However, there was no report of colonization of free-living amoebae in healthy individuals in Iran. The present study was conducted to investigate the possibility that FLAs are the normal flora of the nasal mucosa. Overall, 46 nasal swab samples were collected from healthy individuals and cultured on a 1.5% non-nutrient agar (NNA) plate enriched with a lawn of heat-killed Escherichia coli. The plates were incubated at room temperature. After one week, the plates were microscopically examined, and this was continued for one month. None of the samples were found positive for FLAs. Our findings may indicate that FLAs are not the normal flora of the nasal mucosa, or their number is so limited in the nasal mucosa that they cannot be isolated by the method used. However, supplementary and comprehensive studies are recommended.
- Free-living amoeba, Healthy individual, Immunocompetent, Acanthamoeba spp
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References
Karamati SA, Niyyati M, Lorenzo-Morales J, Lasjerdi Z. Isolation and molecular characterization of Acanthamoeba genotypes isolated from soil sources of public and recreational areas in Iran. Acta parasitologica. 2016;61(4):784-9.
Niyyati M, Naghahi A, Behniafar H, Lasjerdi Z. Occurrence of Free-living Amoebae in Nasal Swaps of Patients of Intensive Care Unit (ICU) and Critical Care Unit (CCU) and Their Surrounding Environments. Iranian journal of public health. 2018;47(6):908.
Lekkla A, Sutthikornchai C, Bovornkitti S, Sukthana Y. Free-living ameba contamination in natural hot springs in Thailand. Southeast Asian journal of tropical medicine and public health. 2005;36:5.
Visvesvara GS, Moura H, Schuster FL. Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunology & Medical Microbiology. 2007;50(1):1-26.
Martinez AJ, Visvesvara GS. Free‐living, amphizoic and opportunistic amebas. Brain Pathology. 1997;7(1):583-98.
Khan NA, Jarroll EL, Paget TA. Acanthamoeba can be differentiated by the polymerase chain reaction and simple plating assays. Current Microbiology. 2001;43(3):204-8.
Cabello-Vílchez AM, Martín-Navarro CM, López-Arencibia A, Reyes-Batlle M, González AC, Guerra H, et al. Genotyping of potentially pathogenic Acanthamoeba strains isolated from nasal swabs of healthy individuals in Peru. Acta tropica. 2014;130:7-10.
Nazar M, Haghighi A, Niyyati M, Eftekhar M, Tahvildar-Biderouni F, Taghipour N, et al. Genotyping of Acanthamoeba isolated from water in recreational areas of Tehran, Iran. Journal of water and health. 2011;9(3):603-8.
Niyyati M, Ebrahimi M, Haghighi A, Haydari S. Isolation and genotyping of Acanthamoeba spp. from recreational soil of parks in Tehran, Iran. Armaghane danesh. 2013;18(7):530-8.
Lawande RV, Abraham SN, John I, Egler LJ. Recovery of soil amebas from the nasal passages of children during the dusty harmattan period in Zaria. American Journal of Clinical Pathology. 1979;71(2):201-3.
Michel R, Röhl R, Schneider H. Isolation of free-living amoebae from nasal mucosa of healthy individuals. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene 1 Abt Originale B, Hygiene. 1982;176(2-3):155-9.
Denet E, Coupat-Goutaland B, Nazaret S, Pélandakis M, Favre-Bonté S. Diversity of free-living amoebae in soils and their associated human opportunistic bacteria. Parasitology research. 2017;116(11):3151-62.
Arab-Mazar Z, Niyyati M, Lasjerdi Z, Spotin A, Darzam IA, Gachkar L. Isolation, identification, and phylogenetic analysis of potentially pathogenic free-living amoebae isolated from nasal and oral mucosa of HIV/AIDS patients in Iran. Parasitology research. 2019;118(10):3061-6.
, Anisah N, Amal H, Kamel A, Yusof S, Noraina A, Norhayati M. Isolation of Acanthamoeba sp. from conjunctival sac of healthy individuals using swab. Trop Biomed. 2005;22(1):11-4.
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