ISSN: 2538-2462
October 2016
Vol. 1 No. 4 (2016)
Purification of Human Serum Albumin by Ion Exchange Chromatography
Journal of Cellular & Molecular Anesthesia,
Vol. 1 No. 4 (2016),
,
Page 158-162
https://doi.org/10.22037/jcma.v1i4.13565
Abstract
Introduction: Albumin, one of the most important plasma proteins, has a difficult process of synthesis and production. We compared two different methods for albumin purification: Carboxymethyl cellulose (CM cellulose) resin exchange and Diethylaminoethyl cellulose (DEAE cellulose) resin exchange in order to determine which resin could be more beneficial.
Materials and methods: two ion exchange resins were used DEAE cellulose resin and CM cellulose resin. All resins were recruited according to the standard preparation protocol. The final results were analyzed using SDS-PAGE technique.
Results: in DEAE Cellulose resin, nearly more than 75% of the purified protein was albumin; while, in CM cellulose resin, more than 90% was albumin.
Conclusion: albumin purification using CM cellulose resin is much more efficacious compared to DEAE cellulose resin. Though significant laboratory findings were demonstrated in this study, clinical studies are needed to confirm clinical outcomes.
- human serum albumin
- carboxymethyl cellulose
- diethylaminoethyl cellulose
- ion exchange chromatography
How to Cite
References
He XM, Carter DC. Atomic structure and chemistry of human serum albumin. Nature. 1992;358(6383):209-15.
Boppana R, Kulkarni RV, Mutalik SS, Setty CM, Sa B. Interpenetrating network hydrogel beads of carboxymethylcellulose and egg albumin for controlled release of lipid lowering drug. Journal of microencapsulation. 2010;27(4):337-44.
Roberts I, Blackhall K, Alderson P, Bunn F, Schierhout G. Human albumin solution for resuscitation and volume expansion in critically ill patients. Cochrane Database Syst Rev. 2011(11):Cd001208.
Nishimura A, Tabuchi Y, Kikuchi M, Masuda R, Goto K, Iijima T. The Amount of Fluid Given During Surgery That Leaks Into the Interstitium Correlates With Infused Fluid Volume and Varies Widely Between Patients. Anesth Analg. 2016.
Sugio S, Kashima A, Mochizuki S, Noda M, Kobayashi K. Crystal structure of human serum albumin at 2.5 A resolution. Protein engineering. 1999;12(6):439-46.
Haefliger DN, Moskaitis JE, Schoenberg DR, Wahli W. Amphibian albumins as members of the albumin, alpha-fetoprotein, vitamin D-binding protein multigene family. Journal of molecular evolution. 1989;29(4):344-54.
Jakus V, Hrnciarova M, Carsky J, Krahulec B, Rietbrock N. Inhibition of nonenzymatic protein glycation and lipid peroxidation by drugs with antioxidant activity. Life sciences. 1999;65(18-19):1991-3.
Salama A, El-Sakhawy M, Kamel S. Carboxymethyl cellulose based hybrid material for sustained release of protein drugs. International journal of biological macromolecules. 2016.
Hoang B, Ernsting MJ, Roy A, Murakami M, Undzys E, Li SD. Docetaxel-carboxymethylcellulose nanoparticles target cells via a SPARC and albumin dependent mechanism. Biomaterials. 2015;59:66-76.
Chen X, Liu L, Jiang C. Charge-reversal nanoparticles: novel targeted drug delivery carriers. Acta pharmaceutica Sinica B. 2016;6(4):261-7.
Zadow JG, Hill RD. The precipitation of proteins by carboxymethyl cellulose. The Journal of dairy research. 1975;42(2):267-75.
Qi Y, Yan Z, Huang J. Chromatography on DEAE ion-exchange and Protein G affinity columns in tandem for the separation and purification of proteins. Journal of biochemical and biophysical methods. 2001;49(1-3):263-73.
Desjardins PR, Rabkin SW, Jacobs HK. DEAE-cellulose chromatography of creatine kinase isoenzymes--effect of pH and serum. Clinical biochemistry. 1979;12(3):77-82.
Barbosa LR, Ortore MG, Spinozzi F, Mariani P, Bernstorff S, Itri R. The importance of protein-protein interactions on the pH-induced conformational changes of bovine serum albumin: a small-angle X-ray scattering study. Biophys J. 2010;98(1):147-57.
Lee SY, Bang S, Kim S, Jo SY, Kim BC, Hwang Y, et al. Synthesis and in vitro characterizations of porous carboxymethyl cellulose-poly(ethylene oxide) hydrogel film. Biomaterials research. 2015;19:12.
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