In vitro maturation of human oocytes using culture in alginate matrix
Men's Health Journal,
Vol. 2 No. 1 (2018),
13 July 2018
Introduction: In vitro maturation (IVM) infertility treatment in women with infertility problems - for example, polycystic ovary syndrome (PCOS) or ovarian hyperstimulation syndrome (OHSS) reduces the amount of gonadotrophin medications, thereby reducing the cost of treatment. This study aimed to investigate the effect of alginate three-dimensional culture on maturity of immature human oocytes and the amount of 8-cell embryos produced by sperm injection to oocyte cytoplasm (ICSI).
Materials and methods: This experimental study was conducted in Mahdieh Infertility Research Center from 2015 to 2017. A total of 70 immature oocytes from the female ovary to candidate for fertilization were selected and divided into two control and experimental groups. In the control group, the basic culture medium (TCM199, FSH 0/75, FBS10 %) and in the experimental group, the basic culture medium with the alginate was used. Then, the mmature oocytes were fertilized by ICSI and 8-cell embryos was evaluated by reverse microscope. Quantitative data was analyzed using the ANOVA statistical method and SPSS software at significant level of a P<0.05.
Results: The amount of maturity of immature oocytes to MII metaphase stage and the arrival of the embryos to the 8-cell significantly increased in alginate group compared to the control group (P<0.001).
Conclusion: Results demonstrated that alginate can increase the maturity of immature human oocytes and also increases the rate of embryos reaching the 8-cell stage.
How to Cite
Farquhar C, Marjoribanks J. Assisted reproductive technology: an overview of Cochrane Reviews. Cochrane Database of Systematic Reviews. 2018(8).
Hatırnaz Ş, Ata B, Hatırnaz ES, Dahan MH, Tannus S, Tan J, et al. Oocyte in vitro maturation: A sytematic review. 2018.
Kedem A, Yerushalmi GM, Brengauz M, Raanani H, Orvieto R, Hourvitz A, et al. Outcome of immature oocytes collection of 119 cancer patients during ovarian tissue harvesting for fertility preservation. Journal of assisted reproduction and genetics. 2018;35(5):851-6.
Bertoldo MJ, Walters KA, Ledger WL, Gilchrist RB, Mermillod P, Locatelli Y. In-vitro regulation of primordial follicle activation: challenges for fertility preservation strategies. Reproductive biomedicine online. 2018.
Mohsenzadeh M, Khalili M, Tabibnejad N, Salehi-Abargouei A, Karimi-Zarchi M, editors. Impact of vitrification on human oocytes before and after in vitro maturation (IVM): a systematic review and meta-analysis. HUMAN REPRODUCTION; 2018: OXFORD UNIV PRESS GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND.
Zhu J-Q, Liu Y, Zhang J-H, Liu Y-F, Cao J-Q, Huang Z-T, et al. Cadmium exposure of female mice impairs the meiotic maturation of oocytes and subsequent embryonic development. Toxicological Sciences. 2018.
Tiwari M, Prasad S, Tripathi A, Pandey AN, Ali I, Singh AK, et al. Apoptosis in mammalian oocytes: a review. Apoptosis. 2015;20(8):1019-25.
Pincus G, Enzmann EV. The comparative behavior of mammalian eggs in vivo and in vitro: I. The activation of ovarian eggs. Journal of Experimental Medicine. 1935;62(5):665-75.
Horn EC. Extranuclear histone in the amphibian oocyte. Proceedings of the National Academy of Sciences of the United States of America. 1962;48(2):257.
Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertility and sterility. 1991;55(1):109-13.
Khashavi Z, Karimzadeh M. Comparison of Unstimulated In Vitro Maturation and Stimulated: In Vitro Fertilization in Women with Poly Cystic Ovarian Syndrome. Journal of Research in Medical Sciences. 2004;9(6):260-3.
Accardo C, Dattena M, Pilichi S, Mara L, Chessa B, Cappai P. Effect of recombinant human FSH and LH on in vitro maturation of sheep oocytes; embryo development and viability. Animal reproduction science. 2004;81(1-2):77-86.
de Matos DG, Gasparrini B, Pasqualini SR, Thompson JG. Effect of glutathione synthesis stimulation during in vitro maturation of ovine oocytes on embryo development and intracellular peroxide content. Theriogenology. 2002;57(5):1443-51.
Cortvrindt R, Smitz J, Van Steirteghem A. Ovary and ovulation: In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system. Human Reproduction. 1996;11(12):2656-66.
West ER, Xu M, Woodruff TK, Shea LD. Physical properties of alginate hydrogels and their effects on in vitro follicle development. Biomaterials. 2007;28(30):4439-48.
Kreeger PK, Deck JW, Woodruff TK, Shea LD. The in vitro regulation of ovarian follicle development using alginate-extracellular matrix gels. Biomaterials. 2006;27(5):714-23.
Brito I, Silva G, Sales A, Lobo C, Rodrigues G, Sousa R, et al. Fibrin–alginate hydrogel supports steroidogenesis, in vitro maturation of oocytes and parthenotes production from caprine preantral follicles cultured in group. Reproduction in Domestic Animals. 2016;51(6):997-1009.
Segers I, Mateizel I, Van Moer E, Smitz J, Tournaye H, Verheyen G, et al. In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising “ex vivo” method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe. Journal of assisted reproduction and genetics. 2015;32(8):1221-31.
Dahan MH, Tan SL, Chung J, Son W-Y. Clinical definition paper on in vitro maturation of human oocytes. Human Reproduction. 2016;31(7):1383-6.
Wei S, Gong Z, Zhao H, Liang H, Lai L, Deng Y. Equine chorionic gonadotropin influence on sheep oocyte in vitro maturation, apoptosis, and follicle-stimulating hormone receptor and luteinizing hormone receptor expression. Genet Mol Res. 2016;15(4):1-12.
Bolamba D, Russ KD, Harper SA, Sandler JL, Durrant BS. Effects of epidermal growth factor and hormones on granulosa expansion and nuclear maturation of dog oocytes in vitro. Theriogenology. 2006;65(6):1037-47.
Xu M, West E, Shea LD, Woodruff TK. Identification of a stage-specific permissive in vitro culture environment for follicle growth and oocyte development. Biology of reproduction. 2006;75(6):916-23.
Xu J, Lawson M, Yeoman R, Pau K, Barrett S, Zelinski M, et al. Secondary follicle growth and oocyte maturation during encapsulated three-dimensional culture in rhesus monkeys: effects of gonadotrophins, oxygen and fetuin. Human Reproduction. 2011;26(5):1061-72.
Laronda MM, Duncan FE, Hornick JE, Xu M, Pahnke JE, Whelan KA, et al. Alginate encapsulation supports the growth and differentiation of human primordial follicles within ovarian cortical tissue. Journal of assisted reproduction and genetics. 2014;31(8):1013-28.
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