Gastroenterology and Hepatology from Bed to Bench https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb <p>The "<em><strong>Gastroenterology and Hepatology From Bed to Bench (GHFBB)</strong></em>" is an open-access, quarterly, multidisciplinary, and medical publication journal published by&nbsp;<a href="http://gldrc.sbmu.ac.ir/?siteid=136&amp;pageid=16100" target="_blank" rel="noopener">Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences</a>&nbsp;(RIGLD), affiliated to Shahid Beheshti University of Medical Sciences. The journal is included papers (original research papers, review articles, case reports, editorials, letters to the editor, and brief reports) in various disciplines of gastroenterology and hepatology from clinical research to basic sciences studies. Editors, with the help of an Editorial Executive Committee, conduct reviews quickly and fairly, and then accepted manuscripts are available online for free.</p> <p><em>Gast</em><em>r</em><em>oente</em><em>r</em><em>olog</em><em>y</em>&nbsp;<em>an</em><em>d Hepatology From Bed to Bench&nbsp;</em>is approved by Iranian Medical Journal Commission, Deputy of Research and Technology, Ministry of Health and Medical Education, Iran.</p> <p><strong>GHFBB is indexed in PMC (PubMed), Scopus, Elsevier products, EMBASE, EBSCO, Index Medicus for the Eastern Mediterranean Region (IMEMR), ISC, IndexCopernicus, Centre for Agriculture and Bioscience International (CABI), Scientific Information Database (SID), Magiran, and some of the indexing process is in progress.<br></strong></p> <p>&nbsp;</p> <p><strong>Editorial Office</strong></p> <p>PO Box: 19835-178</p> <p>Fax: +982122432517&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p> <p>Tel: +982122432525&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p> <p>E-mail:&nbsp;<a href="mailto:ghfbb.journal@gmail.com">ghfbb.journal@gmail.com</a></p> <p>Address: Research Institute for Gastroenterology and Liver Diseases (RIGLD), Aerabi St., Yemen St., Chamran Highway, Tehran, &nbsp;Iran.</p> Publisher: Research Institute for Gastroenterology and Liver Diseases (RIGLD) en-US Gastroenterology and Hepatology from Bed to Bench 2008-2258 <div>Copyright: By submitting their articles to this journal, authors will transfer the copyright of their published work to GHFBB.</div> <div>No Prior or Subsequent Publication: Along with submitting a manuscript for consideration, the authors should confirm in writing that “neither the submitted paper nor any similar paper, in whole or in part, other than an abstract or preliminary communication, has been or will be submitted to or published in any other scientific journal”.</div> <div>Requests for permission to reproduce all or parts of articles published in GHFBB will be processed, however, in GHFBB editorial office [Phone: (+98-21) 22432515; Fax: (+98-21) 22432517; e-mail: ghfbb.journal@gmail.com]</div> <div>Embargo Policy: Contents of submitted works cannot be discussed with the media (including other scientific journals) until 12:00 A.M. of publication date.</div> Gastroenterology and Hepatology from Bed to Bench (GHFBB) from a bibliometric perspective (2011-2021) https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2621 Abedin Hosseini Ahangari Davoud Haseli Heidar Mokhtari Mohammad Rostami Nejad Mohammad Ahmadian Mohammad Karim Saberi Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2621 Pathophysiological mechanisms of cardiovascular disorders in non-alcoholic fatty liver disease https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2549 <p>Non-alcoholic fatty liver disease is one of the main liver diseases worldwide. The most common cause of death in patients with non-alcoholic fatty liver disease is cardiovascular diseases. Currently, the relationship between them is well established. Indeed, identical reasons may contribute to the development of cardiovascular diseases and non-alcoholic fatty liver disease, with lifestyle factors such as smoking, sedentary lifestyle with poor nutrition habits, and physical inactivity being major aspects. This review focuses on potential pathophysiological mechanisms of cardiovascular disorders in non-alcoholic fatty liver. PubMed, EMBASE, Orphanet, MIDLINE, Google Scholar, and Cochrane Library were searched for articles published between 2006 and 2022. Relevant articles were selected by using the following terms: “Non-alcoholic fatty liver disease”, “Сardiovascular diseases”, “Pathophysiological mechanisms”. Then the reference lists of identified articles were searched for other relevant publications as well. The pathophysiological mechanisms of cardiovascular disorders in non-alcoholic fatty liver remain largely speculative and may include systemic low-grade inflammation, atherogenic dyslipidemia, abnormal glucose metabolism and hepatic insulin resistance, endothelial dysfunction, gut dysbiosis, as well as the associated cardiac remodeling, which are influenced by interindividual genetic and epigenetic variations. It is clear that the identification of pathophysiological mechanisms underlying cardiovascular disorders in non-alcoholic fatty liver disease will make the choice of therapeutic measures more optimal and effective.</p> Dmitry Garbuzenko Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2549 An update: the comparison of Portal Hypertensive Gastropathy and Gastric Antral Vascular Ectasia https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2561 <p>Portal hypertensive gastropathy (PHG) and gastric antral vascular ectasia (GAVE) are two distinct entities that are frequently mistaken with each other, because they present with similar manifestations. This issue may cause catastrophic outcomes, since each one of them has a unique pathophysiology and thereby their management is totally different. There are clinical clues that helps the physicians to distinguish these two. Upper endoscopy and biopsy are often required to establish the diagnosis. In this review, we ought to discuss about different aspects of the both conditions and highlight the clinical evidences that may help us identify the disease and manage it appropriately.</p> Mohsen Rajabnia Behzad Hatami Pardis Ketabi Moghadam Mahsa Mohammadi Mitra Rafizadeh Forogh Mangeli Mobin Fathi Ali Jahanian Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2561 An Updated Review on Drug-Induced Esophagitis and A Helpful Management for Healthcare Providers https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2591 <p>In recent decades, the number of cases developing drug-induced esophagitis (DIE) has been identified to an increasing extent, which shows the significance of detecting medicines capable of causing this adverse reaction. This study aims to provide an updated review on recent case reports of DIE, evaluate the possible mechanism of this side effect, and provide helpful management. Data was gathered through searching three databases, including PubMed, Medline, and Cochrane. Seven drug categories were evaluated, including antibiotics, non-steroidal anti-inflammatory drugs (NSAIDs), cardiovascular medicines, bisphosphonates, chemotherapeutic agents, miscellaneous agents, and supplements. According to our findings, retrosternal pain, heartburn, odynophagia, and dysphagia are the typical symptoms. In most cases, DIE is a self-limiting side effect that can resolve by removing the causative agent and supportive therapy.</p> Saeed Abdi Farnoosh Masboogh Maryam Nazari Mohammad Abbasinazari Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2591 Sequence variations of Epstein–Barr virus LMP1 gene in gastric cancer and chronic gastritis isolates from Iranian patients https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2578 <p><strong>Aim:</strong> The aim of this study was to investigate sequence variations in the C-terminus of latent membrane protein 1 (LMP1) in Epstein-Barr virus (EBV) isolates from Iranian patients with chronic gastritis or gastric cancer (GC).</p> <p><strong>Background:</strong> EBV is an important member of gammaherpesviruses that causes persisting latent human infection. LMP1 is the essential viral oncoprotein that is a key element for B cells immortalization. LMP1 contains of a small twenty-four amino acid cytoplasmic N-terminal region, six transmembrane segments and a two hundred amino acid cytoplasmic C-terminal domain. Most LMP1-mediated signal transduction events are moderated by some functional parts of cytoplasmic C-terminal domain. Sequence variations of LMP1 gene have been described in numerous EBV-isolates.</p> <p><strong>Methods:</strong> This study included 32 EBV positive biopsy tissues from patients with gastric cancer and patients with chronic gastritis. The C-terminal nucleotide sequences of LMP1 were amplified using nested-PCR and analyzed by DNA sequencing.</p> <p><strong>Results:</strong> In the carboxyl-terminal site of patients was observed 4 to 8 copies of the 11 repeat elements (codon 254–302) and there was no relationship between the number of repeat sequences and disease status. The 30-bp deletion corresponding to codon 345–354 of the B95-8 strain was observed in 34% isolates and remaining samples were non-deleted. In group of gastric cancer, a high number of 33-bp repeats (&gt;5 repeats) was observed in 30-bp-deletion (100%) than non-deleted (42%) isolates and the difference was also statistically significant. In addition, the analysis revealed that a gastritis isolate may be result of recombination between Alaskan and China1 strains.</p> <p><strong>Conclusion:</strong> Overall, our results showed no association between the C-terminal sequence variations of LMP1 and malignant or non-malignant isolate origin.</p> Behrang Sarshari Seyed Reza Mohebbi Mehrdad Ravanshad Shabnam Shahrokh Mohammad Reza Zali Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2578 Bioinformatics-based Identification of miRNAs, mRNA and regulatory signaling pathways involved in esophageal squamous cell carcinoma https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2465 <p>The most common form of esophageal cancer is esophageal squamous cell carcinoma (ESCC). miRNAs are well recognized as having a critical regulatory role in human cancer, but their responsibilities and mechanisms of miRNA-mRNA in ESCC are yet unknown. As a result, the miRNA microarray dataset (GSE66274) and gene expression microarray dataset (GSE38129) with similar samples were analyzed to better understand the miRNA-mRNA interactions. The differentially expressed miRNAs (DEmiRNAs) and differentially expressed mRNAs (DEmRNAs) were identified using the LIMMA package in R. In total, 478 DEmRNA (224 up-regulated &amp; 254 down-regulated) and 39 DEmiRNA (15 up-regulated &amp; 24 down-regulated) were screened out. miRNA-mRNA interactions were analyzed by RNAInter database, then the miRNA-mRNA network was visualized by Cytoscape software. On the other hand; ClusterProfiler packages were used to perform gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses for DEmRNA as targets of DEmiRNAs. KEGG pathway analysis indicated that the p53 signaling pathway, ECM−receptor interaction, and AGE−RAGE signaling pathway were significant. Besides, cellular response to amino acid stimulus, negative regulation of apoptotic signaling pathway, and endoderm formation was most prevalent in the biological process category. Additionally, the collagen−containing extracellular matrix, actomyosin complex collagen trimers and basement membrane, and extracellular matrix structural constituent were more enriched. Taken together, the present survey provided evidence that could support the prognosis of esophageal tumors in the future.</p> Nahid Askari Morteza Hadizadeh Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2465 Efficacy and Safety of Acetylcysteine for the Prevention of Liver Injury in Covid-19 Intensive Care Unit Patients Under Treatment with Remdesivir: A Double-Blind, Placebo-Controlled Randomized Clinical Trial https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2565 <p><strong>Aim:</strong> The objective of this double-blinded placebo-controlled randomized clinical trial was to evaluate prophylactic use of acetylcysteine for prevention of liver injury in patients with severe COVID-19 pneumonia under treatment with remdesivir.</p> <p><strong>Background: </strong>Liver injury is reportedly common in patients with severe COVID-19 pneumonia, and can occur not only as a result of disease progression but as an iatrogenic reaction to remdesivir.</p> <p><strong>Methods:</strong> A total of 83 adult patients with severe COVID-19 pneumonia were randomly assigned in parallel groups to receive either acetylcysteine or placebo. All the patients received standard care according to institutional protocols including remdesivir for a total of five days. One gram acetylcysteine was administered intravenously every 12 hours for 42 patients, and 41 patients received the same volume of 0.9% sodium chloride as placebo.</p> <p><strong>Results:</strong> After 5 days, median aspartate transaminase (AST) and alanine transaminase (ALT) levels were significantly lower in the acetylcysteine than in the placebo group. Of those who received placebo, 30 (73.2%), 4 (9.7%) and 3 (7.3%) patients had serum AST levels elevated between 1-2.5, 2.5-5 and over 5 times the upper limit of normal (ULN), respectively; while in the acetylcysteine group, 33 (78.6%) and 0 patients had AST levels between 1-2.5 and over 2.5 times ULN, respectively (p-value=0.037). In the acetylcysteine group, 23 (54.8%), 1 (2.4%) and 1 (2.4%) patients had serum ALT levels elevated between 1-2.5, 2.5-5 and over 5 times ULN, respectively; while in the placebo group, 24 (58.5%), 7 (17.1%) and 1 (2.4%) patients had serum ALT levels between 1-2.5, 2.5-5 and over 5 times ULN, respectively (p-value=0.073).</p> <p><strong>Conclusion: </strong>Intravenous administration of acetylcysteine significantly prevents liver transaminases elevation and liver injury in seriously ill COVID-19 patients treated with remdesivir. (Trial Registration: www.irct.ir identifier, IRCT20210726051995N1)</p> Pouria Mousapour Ramin Hamidi Farahani Reza Mosaed Ali Asgari Ebrahim Hazrati Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2565 Down-regulation of TP53 Is a Highlighted Molecular Event in Gastric Ulcer https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2544 <p><strong>Background</strong>:&nbsp; Gastric ulcer as an acid related gastrointestinal disease is known as a one of the most public gastrointestinal disorders.</p> <p><strong>Aim:</strong> Explore the crucial dysregulate proteins and biochemical pathways in gastric ulcer is the main aim of this projects.</p> <p><strong>Methods</strong>: Number of 100 proteins from STRING database were analyzed by cytoscape and its applications to find the central proteins and also the related biochemical pathways. Action map analysis was applied to explore regulatory relationship between the critical proteins.</p> <p><strong>Results</strong>: Common results of network analysis and gene ontology revealed that IL6, ALB, TNF, INS, IL1B, IL10, TP53, CXCL8, and PTGS2 are the highlighted related proteins in gastric ulcer. Six clusters of biochemical pathways including “Response to external stimulus”, “multicellular organismal process”, “regulation of biological quality”, “cellular response to stimulus”, “cellular response to chemical stimulus”, and “transport” were identified as the dysregulated pathway in patients.</p> <p><strong>Conclusion</strong>: It seems that down-regulation of TP53 by IL2, PTGS2, and TNF is a main process that occurs in patients. &nbsp;</p> Reza Vafaee Somayeh Jahani Sherafat Mostafa Rezaei –Tavirani Nayebali Ahmadi Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2544 Prevalence of Enteric Adenovirus Gastroenteritis and Co-infection with Rotavirus in under-Five-Year-Old Children, Qom-Iran https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2483 <p><strong>Aim: </strong>The study was performed to determine the prevalence and co-infections with Rotavirus in children under five years of age.</p> <p><strong>Background</strong>: Gastroenteritis-associated&nbsp;viral infections are a cause of death among young children in Worldwide, especially in developing countries. The species F Adenovirus (40 and 41) is responsible for rang of the cases of acute diarrhea among infants children.</p> <p><strong>Methods: </strong>During 9 months, 130 children with intestinal symptoms referred to the pediatric ward of the hospital were enrolled in this study. After collecting fecal samples, viral genomes were extracted; and then amplified and typed using polymerase chain reaction by Adenovirus-specific primers. Rotavirus was detected in our previous study on the same samples and the results were used to evaluate for co-infection<strong>. </strong></p> <p><strong>Results:</strong> Mean&nbsp;age&nbsp;of the patients was 32.09±32.68 months. 60% and 40% of the patients were males and females, respectively. Adenovirus infection was identified among 23 cases of the children (17.7%), 21 cases (91%) type 41 and 2 cases (9%) type 40. Fever was the most clinical manifestation and there&nbsp;were&nbsp;no significant&nbsp;relations between&nbsp;the clinical symptoms and the Adenovirus infection. Co-infection was found in only 5 cases (21.7%) of patients.</p> <p><strong>Conclusion:</strong> These data show that Adenovirus infection plays an important role in acute diarrheal infection in Qom. In addition, our findings indicated that there was a co-infection between Adenovirus and Rotavirus, stating a serious problem in children less than five years of age.</p> Saeed Shams Javad Tafaroji Mohammad Aghaali Nayebali Ahmadi Hosein Heydari Seyed Dawood Mousavi Nasab Vineet Kumar Maurya Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2483 Detoxification of aflatoxin M1 from the reconstituted milk by probiotics Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus, using the HPLC method https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2402 <p><strong>Abstract</strong></p> <p>Aflatoxins are poisonous substances produced by certain kinds of fungi that are found naturally all over the world. They can&nbsp;contaminate food crops&nbsp;and pose a serious health threat to humans and livestock. The current study aimed at removing aflatoxin from the reconstituted milk by adding three probiotics <em>Saccharomyces boulardii</em>, <em>Lactobacillus</em><strong> <em>casei</em></strong> and <em>Lactobacillus</em> <strong><em>acidophilus</em></strong><strong>.</strong></p> <p><strong>Materials and Methods</strong></p> <p>The probiotics of <em>S. boulardii</em>&nbsp;<em>, </em><em>L.</em><em>casei</em> and <em>L.<strong> acidophilus</strong></em> with 10<sup>9</sup> and 10<sup>7 </sup>CFU concentration were exposed to aflatoxin M1 (0.5 and 0.75 ng/ml). The ELISA test was performed using 144 falcon tubes containing AFM1. Sterile water was added to each probiotic pellet and finally added to pre-prepare contaminated milk. After the specified times, the milk layer was analyzed to measure AFM1 levels. Each sample was analyzed using HPLC system. Subsequently, the percentage of AFM1, which was bound to the bacterial suspension, was calculated.</p> <p><strong>Results</strong></p> <p><em> boulardii</em> had the greatest ability in AFM1 removal from milk medium (96.88 ± 3.79) over time in the early hours with increasing concentration of AFM1 (0.75 ng/ml) and a concentration of 10<sup>9</sup> CFU/ml at 37 °C. The highest activity of <em>L.casei </em>in the removal of AFM1 toxin was observed at a concentration of 10<sup>7</sup> CFU/ml in 0.75 ng/ml AFM1 level and 37 °C. And the highest marginal estimation percentage of AFM1 removal from the milk medium at 4 °C in initial minutes belonged <em>to L.</em> <strong><em>acidophilus.</em></strong></p> <p><strong>Conclusion</strong></p> <p>The results revealed the possibility of using <em>S. boulardii </em>in combination with selected strains of LAB (<em>L.casei</em>, <em>L.<strong> acidophilus</strong></em>) in detoxification of AFM1-contaminated milk.</p> Sama Rezasoltani Niloufar Amir Ebrahimi Reza Khadivi Boroujeni Hamid Asadzadeh Aghdaei Mohsen Norouzinia Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2402 Education case: A case-based approach to overlap autoimmune liver disease in patient with ulcerative colitis https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2332 <p><strong>Abstract</strong></p> <p>Simultaneous&nbsp;occurrence&nbsp;of&nbsp;immune-based&nbsp;gastrointestinal diseases and&nbsp;autoimmune hepatitis although is not common but is of clinical importance. Some clinical and laboratory findings such as severe pruritus and an elevation in alkaline phosphatase raise suspicion of a biliary disease which overlaps autoimmune hepatitis. A strong clinical suspicion of overlap syndrome in a patient with autoimmune hepatitis prompts more diagnostic evaluations like MRCP, liver biopsy and secondary laboratory tests. &nbsp;Patients who fall into the category of overlap syndrome would be proceeded with timely &nbsp;monitoring&nbsp;of&nbsp;known&nbsp;complications including colorectal carcinomas, cholangiocarcinomas and gallbladder cancers. So, it is highly recommended to search for all simultaneous immune-based involvements prior to labelling a patient as having pure autoimmune hepatitis.</p> <p>In&nbsp;this study,&nbsp;attempts have been made to express all challenges about a case with overlap syndrome referred to gastroenterology ward of Taleghani hospital and &nbsp;review&nbsp;the&nbsp;latest&nbsp;articles&nbsp;and&nbsp;related&nbsp;guidelines&nbsp;about the diagnosis, treatment, complications, and surveillance of the mentioned patient&nbsp;with&nbsp;autoimmune&nbsp;hepatitis (AIH),&nbsp;primary&nbsp;sclerosing&nbsp;cholangitis (PSC),&nbsp;and&nbsp;inflammatory&nbsp;bowel&nbsp;disease (IBD).</p> <p><strong>Keywords:</strong> Autoimmune&nbsp;Hepatitis,&nbsp;Primary&nbsp;Sclerosing&nbsp;Cholangitis,&nbsp;Inflammatory&nbsp;Bowel&nbsp;Disease, Ulcerative Colitis, overlap syndrome.</p> Amir Sadeghi Pardis Ketabi Moghadam Forough Mangeli Niloufar Salehi Mohsen Rajabnia Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2332 Plexiform Neurofibromatosis of the Liver: An Extremely Rare Case https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2047 <p>Herein, we report an extremely rare case of histopathologically proven neurofibromatosis of the liver. A 15-year-old male, a known case of type I neurofibromatosis (NF1), referred to our hospital with a complaint of right upper quadrant pain. He had a café-au-lait spot and positive family history of NF1 in her mother. Laboratory data were within normal limits, and computed tomography (CT) revealed a large predominantly less attenuated infiltrative liver mass along the porta hepatis with extension to both lobes of the liver. Moreover, magnetic resonance imaging showed a large hypo signal mass in T1-weighted images and hypersignal lesion in T2-sequences with faint enhancement, periportal distribution, and encasing of major branches of the portal vein without evidence of narrowing and invasion. Furthermore, the CT guided biopsy was taken from both liver lobe lesion, and pathological diagnosis of the biopsy specimens confirmed plexiform neurofibromas of the liver. According to the extensive intrahepatic extension and periportal infiltration, the mass was unrespectable. Therefore, the radiologists need to be familiar with the typical imaging features of the uncommon hepatic neoplasms. If imaging findings are not typical or diagnostic, a further biopsy should be performed once again.</p> Farideh Gharekhanloo Saba Lorestani Salman Khazaei Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2047 Not Every Pancreatic Mass is Cancer: A Case of a Large Intra-Pancreatic Splenule https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2539 <p>We report a case of a 72-year-old man who was referred to our tertiary medical center for endoscopic ultrasound (EUS) evaluation for an incidental 2 cm mass in the tail of the pancreas seen on computed tomography (CT).&nbsp; On EUS, a 22 mm by 13 mm, well-defined hypoechoic mass was identified within the pancreatic tail and a fine needle biopsy was performed. Histopathology revealed benign pancreatic parenchyma and the presence of lymphocytes. A technetium-99m sulfur colloid scan was performed which demonstrated uptake in the pancreatic tail lesion, consistent with an intra-pancreatic splenule. This case demonstrates that a splenule or accessory splenic tissue should remain in the differential diagnosis of a pancreatic mass. An accurate diagnosis of pancreatic splenule can preclude surgical resection.&nbsp;</p> Nicholas McDonald Daniyal Abbas Mohammad Bilal Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2539 Primary squamous cell carcinoma of the stomach: A rarity https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2609 <p>Introduction: Squamous cell carcinoma of the stomach is of rare occurrence with only a few cases reported in the medical literature. <br>Case: We hereby present the case of a 73 year old male who presented to us with complains of abdominal pain, generalized weakness along with weight loss. His upper and lower GI endoscopies revealed no visible abnormality, following which a CT CAP was done and showed a soft tissue mass involving the stomach. EUS guided biopsies were done and revealed moderate to poorly differentiated squamous cell carcinoma. Later on radial EUS was done for staging purpose and showed T2/3, N1 disease. He was referred to the oncology team where he was administered chemotherapy on the lines of squamous cell carcinoma and is currently on regular follow-up.<br>Conclusion: Our case shows that in spite of having a poor come, early identification may have better consequences in the long run for these patients.</p> Zain Majid Danish Ratani Abbas Ali Tasneem Syed Mudassir Laeeq Nasir Luck Muhammad Mubarak Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench 2022-06-20 2022-06-20 10.22037/ghfbb.v15i3.2609 Analysis of protein-protein interaction of digestive disorders and Covid-19 virus: correspondence https://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/view/2636 <p>n/a</p> Rujittika Mungmunpuntipantip Viroj Wiwanitkit Copyright (c) 2022 Gastroenterology and Hepatology from Bed to Bench https://creativecommons.org/licenses/by-nc/4.0/ 2022-07-31 2022-07-31 10.22037/ghfbb.v15i3.2636