Publisher: Research Institute for Gastroenterology and Liver Diseases (RIGLD)
  • Register
  • Login

Gastroenterology and Hepatology from Bed to Bench

  • Home
  • Issues
    • Current
    • Archives
  • About
    • About the Journal
    • Aims and Scope
    • Editorial Team
    • Privacy Statement
    • Contact
  • For Authors
    • Submissions
    • Author Guidelines
    • Peer Review Process
  • Indexing & Abstracting
  • Announcements
Advanced Search
  1. Home
  2. Archives
  3. Vol. 3 No. 1 (2010): Winter
  4. New Section Title Here

ISSN: 2008-2258

Winter
Vol. 3 No. 1 (2010)

Characterization of Entamoeba histolytica and Entamoeba dispar in fresh stool by PCR

  • Ehsan Nazemalhosseini Mojarad
  • Zahra Nochi
  • Navid Sahebekhtiari
  • Mohammad Rostami Nejad
  • Hossein Dabiri
  • Ali Haghighi

Gastroenterology and Hepatology from Bed to Bench, Vol. 3 No. 1 (2010),
https://doi.org/10.22037/ghfbb.v3i1.68 Published 12 December 2009

  • View Article
  • Download
  • Cite
  • Statastics
  • Share

Abstract

Aim: The aim of this study was to differentiate Entamoeba dispar from Entamoeba histolytica by PCR directly from fresh stool.

Background: Microscopy does not allow for the differentiation of Entamoeba dispar from Entamoeba histolytica. Several PCR-based methods have been described and used successfully for this purpose, but the methods for DNA extraction from stool samples are usually time-consuming and problematic due to inhibitory factors in feces.  

Patients and methods: From a total of 1700 stool samples collected and examined by microscopy, 22 samples (1.3%) were microscopically positive for the E. histolytica /E. dispar complex. The DNA of these samples was extracted directly from fresh stool and PCR was carried out using two sets of species-specific primers from a short tandem repeat (STR) in the D-A locus.

Results: Of these, 21 samples (95.45%) were diagnosed as E. dispar and only one sample (4.55 %) was found to be E. histolytica. In this study, by improving the DNA extraction from fresh stool, we were able to efficiently differentiate E. histolytica and E. dispar.

Conclusion: To avoid unnecessary treatment of patients not infected with E. histolytica, the development of effective techniques, such as direct DNA extraction from stool, is recommended.

Keywords:
  • Entamoeba histolytica
  • Entamoeba dispar
  • fresh stool
  • PCR
  • locus D-A
  • PDF

How to Cite

Nazemalhosseini Mojarad, E., Nochi, Z., Sahebekhtiari, N., Rostami Nejad, M., Dabiri, H., & Haghighi, A. (2009). Characterization of Entamoeba histolytica and Entamoeba dispar in fresh stool by PCR. Gastroenterology and Hepatology from Bed to Bench, 3(1). https://doi.org/10.22037/ghfbb.v3i1.68
  • ACM
  • ACS
  • APA
  • ABNT
  • Chicago
  • Harvard
  • IEEE
  • MLA
  • Turabian
  • Vancouver
  • Endnote/Zotero/Mendeley (RIS)
  • BibTeX
  • Abstract Viewed: 200 times
  • PDF Downloaded: 207 times

Download Statastics

  • Linkedin
  • Twitter
  • Facebook
  • Google Plus
  • Telegram
Open Journal Systems
Keywords
Current Issue
  • Atom logo
  • RSS2 logo
  • RSS1 logo
  • Home
  • Archives
  • Submissions
  • About the Journal
  • Editorial Team
  • Contact

GHFBB journal is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).

Print ISSN: 2008-2258
Online ISSN: 2008-4234

Support Contact: ghfbb.journal@gmail.com

 

GHFBB is an open-access journal and does not charge fees for authors who submit their articles and for readers who access PDF files of published articles.

The template of this website is designed by Sinaweb