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Vol. 3 No. 1 (2010)

December 2009

Characterization of Entamoeba histolytica and Entamoeba dispar in fresh stool by PCR

  • Ehsan Nazemalhosseini Mojarad
  • Zahra Nochi
  • Navid Sahebekhtiari
  • Mohammad Rostami Nejad
  • Hossein Dabiri
  • Ali Haghighi

Gastroenterology and Hepatology from Bed to Bench, Vol. 3 No. 1 (2010), 12 December 2009
https://doi.org/10.22037/ghfbb.v3i1.68 Published: 2009-12-12

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Abstract

Aim: The aim of this study was to differentiate Entamoeba dispar from Entamoeba histolytica by PCR directly from fresh stool.

Background: Microscopy does not allow for the differentiation of Entamoeba dispar from Entamoeba histolytica. Several PCR-based methods have been described and used successfully for this purpose, but the methods for DNA extraction from stool samples are usually time-consuming and problematic due to inhibitory factors in feces.  

Patients and methods: From a total of 1700 stool samples collected and examined by microscopy, 22 samples (1.3%) were microscopically positive for the E. histolytica /E. dispar complex. The DNA of these samples was extracted directly from fresh stool and PCR was carried out using two sets of species-specific primers from a short tandem repeat (STR) in the D-A locus.

Results: Of these, 21 samples (95.45%) were diagnosed as E. dispar and only one sample (4.55 %) was found to be E. histolytica. In this study, by improving the DNA extraction from fresh stool, we were able to efficiently differentiate E. histolytica and E. dispar.

Conclusion: To avoid unnecessary treatment of patients not infected with E. histolytica, the development of effective techniques, such as direct DNA extraction from stool, is recommended.

Keywords:
  • Entamoeba histolytica
  • Entamoeba dispar
  • fresh stool
  • PCR
  • locus D-A
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How to Cite

Nazemalhosseini Mojarad, E., Nochi, Z., Sahebekhtiari, N., Rostami Nejad, M., Dabiri, H., & Haghighi, A. (2009). Characterization of Entamoeba histolytica and Entamoeba dispar in fresh stool by PCR. Gastroenterology and Hepatology from Bed to Bench, 3(1). https://doi.org/10.22037/ghfbb.v3i1.68
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GHFBB journal is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).

Print ISSN: 2008-2258
Online ISSN: 2008-4234

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