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  3. Vol 8, No 3 (2015): Summer
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ISSN: 2008-2258

Vol 8, No 3 (2015): Summer

Phylogenetic groups, extended-spectrum B-lactamases and metallo-?-lactamase in Escherichia coli isolated from fecal samples of patients with diarrhea in Iran

  • Hesam Alizade
  • Fatemeh Fallah
  • Reza Ghanbarpour
  • Mohammad Reza Aflatoonian
  • Hossein Goudarzi
  • Hamid Sharifi

Gastroenterology and Hepatology from Bed to Bench, , , Page 207-214
https://doi.org/10.22037/ghfbb.v8i3.647 Published 30 July 2015

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Abstract

Aim: The aims of this study were to determine the phylogenetic groups and investigate phenotypic and genotypic of ESBL and MBL-producing E. coli isolates from patients with diarĀ­rhea in Kerman, southeast of Iran.

Background: Escherichia coli is detected as a significant cause of sporadic and outbreaks of diarrhea. The emergence of extended-spectrum ?-lactamases (ESBLs) and Metallo-?-lactamases (MBLs)-producing Escherichia coli caused problems in antibiotic treatments. E. coli strains can be assigned to four main phylogenetic groups, A, B1, B2 and D.

Patients and methods: Two hundred sixteen E. coli isolates were obtained from diarrheal patients between June to December 2013. Screening and confirmation tests of ESBLs and MBLs were done by disk-diffusion and broth micro-dilution methods according to CLSI 2013. The ESBL-positive isolates were screened to determine the phylogenetic groups and presence of blaCTX-M-15, blaOXA-1, blaPER-1, blaVIMand blaIMP genes by PCR.

Results: In this study, From the 216 E. coli isolates 56 ESBL-positive isolates were detected. Among ESBL-positive isolates, 51 isolates were positive for blaCTX-M-15 and one isolate were positive for both blaCTX-M-15 and blaOXA-1 genes. None of isolates were positive for blaPER-1, blaVIMand blaIMP genes. PCR assays for phylotyping of diarrhea isolates indicated that the isolates were belonged to group A (54.16%), B1 (11.11%), B2 (12.96%) and D (21.75%). ESBL-positive isolates mostly distributed in phylo-groups A and D. The isolates possessed blaCTX-M-15 gene were belonged to A (35 isolates), B1 (5), B2 (3) and D (8) phylo-groups. According to the antimicrobial susceptibility results, imipenem was found to be the most active agent, while resistance to cefotaxime was high.

Conclusion: Our results indicate that blaCTX-M-15 gene is widespread among diarrhea. ESBL-producing E. coli isolates were dissemination among diversity of phylo-groups. The adequate infection control measures are essential to prevent further dissemination of ?-lactamases and MBLs in our country.

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How to Cite

Alizade, H., Fallah, F., Ghanbarpour, R., Aflatoonian, M. R., Goudarzi, H., & Sharifi, H. (2015). Phylogenetic groups, extended-spectrum B-lactamases and metallo-?-lactamase in Escherichia coli isolated from fecal samples of patients with diarrhea in Iran. Gastroenterology and Hepatology from Bed to Bench, 8(3), 207-214. https://doi.org/10.22037/ghfbb.v8i3.647
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GHFBB journal is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).

Print ISSN: 2008-2258
Online ISSN: 2008-4234

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