Gastroenterology and Hepatology from Bed to Bench

Vol. 16 No. 1 (2023)

Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene Development of a semi-nested PCR test for surface gene amplification

Gastroenterology and Hepatology from Bed to Bench, Vol. 16 No. 1 (2023), 2 Dey 2023
https://doi.org/10.22037/ghfbb.v16i1.2583

Abstract

Aim: Development of an amplification method for further investigation of HBV S gene variation patterns.

Background: Pre-S/S variants in patients with chronic HBV infection may contribute to the progression of liver damage and Hepatocellular carcinoma (HCC).

Methods: This study was performed on ten patients with chronic HBV infection. Viral DNA was extracted from patient's plasma, primer design was performed, and a semi-nested PCR method was set up to amplify the pre-S/S region of HBV genome. Subsequently, sequencing was performed to analyze the variants of this region.

Results: In the current study, the semi-nested PCR method was successfully set up, and types of variation in the studied samples were investigated.

Conclusion: Pre-S/S variants should be routinely determined in HBV carriers to help identify individuals who may be at a high risk of less favorable liver disease progression. This study showed that the technique could accurately amplify the pre-S/S region, and the product can be successfully used for variation detection by direct sequencing.

Keywords:
  • Hepatitis B virus
  • HBsAg gene
  • PreS variations
  • Semi Nested PCR

How to Cite

Mokhtari, F. ., Kaboosi, H. ., Mohebbi, S. R., Asadzadeh Aghdaei, H. ., & Zali, M. R. . (2022). Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene: Development of a semi-nested PCR test for surface gene amplification. Gastroenterology and Hepatology from Bed to Bench, 16(1). https://doi.org/10.22037/ghfbb.v16i1.2583

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