Development and evaluation of a semi-nested PCR technique for amplification and determination of different surface gene variation patterns in patients with chronic HBV infection: development of an amplification method for HBV S gene Development of a semi-nested PCR test for surface gene amplification
Gastroenterology and Hepatology from Bed to Bench,
Aim: Development of a semi nested PCR method for HBV Surface gene amplification
Background: Pre-S/S variants in patients with chronic HBV infection may contribute to the progression of liver damages and HCC.
Methods: This study was performed on 10 patients with chronic HBV infection. DNA was extracted from patients' blood, primer design was performed, and a semi nested PCR method was used to amplify preS/S region. Subsequently, sequencing was performed to analyze the variants of this region.
Results: In the current study, the semi nested PCR method was successfully set up and the variants such as deletion in the studied samples were investigated.
Conclusion: Pre-S/S variants should be routinely determined in HBV carriers to help identify individuals who may be at a high risk of less favorable liver disease progression and the technique we used in this field has been useful and can lead to accurate analysis of the pre-S/S region in terms of variants.
- Hepatitis B virus
- HBsAg gene
- PreS variations
- Semi Nested PCR
How to Cite
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