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  3. Vol 15, No 3 (2022): Summer
  4. Original Article

ISSN: 2008-2258

Vol 15, No 3 (2022): Summer

Sequence variations of Epstein–Barr virus LMP1 gene in gastric cancer and chronic gastritis isolates from Iranian patients EBV LMP1 variations in GC and chronic gastritis patients

  • Behrang Sarshari
  • Seyed Reza Mohebbi
  • Mehrdad Ravanshad
  • Shabnam Shahrokh
  • Mohammad Reza Zali

Gastroenterology and Hepatology from Bed to Bench, ,
https://doi.org/10.22037/ghfbb.v15i3.2578 Published 20 June 2022

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Abstract

Aim: The aim of this study was to investigate sequence variations in the C-terminus of latent membrane protein 1 (LMP1) in Epstein-Barr virus (EBV) isolates from Iranian patients with chronic gastritis or gastric cancer (GC).


Background: EBV is an important member of gammaherpesviruses that causes persisting latent human infection. LMP1 is the essential viral oncoprotein that is a key element for B cells immortalization. LMP1 contains of a small twenty-four amino acid cytoplasmic N-terminal region, six transmembrane segments and a two hundred amino acid cytoplasmic C-terminal domain. Most LMP1-mediated signal transduction events are moderated by some functional parts of cytoplasmic C-terminal domain. Sequence variations of LMP1 gene have been described in numerous EBV-isolates.


Methods: This study included 32 EBV positive biopsy tissues from patients with gastric cancer and patients with chronic gastritis. The C-terminal nucleotide sequences of LMP1 were amplified using nested-PCR and analyzed by DNA sequencing.


Results: In the carboxyl-terminal site of patients was observed 4 to 8 copies of the 11 repeat elements (codon 254–302) and there was no relationship between the number of repeat sequences and disease status. The 30-bp deletion corresponding to codon 345–354 of the B95-8 strain was observed in 34% isolates and remaining samples were non-deleted. In group of gastric cancer, a high number of 33-bp repeats (>5 repeats) was observed in 30-bp-deletion (100%) than non-deleted (42%) isolates and the difference was also statistically significant. In addition, the analysis revealed that a gastritis isolate may be result of recombination between Alaskan and China1 strains.


Conclusion: Overall, our results showed no association between the C-terminal sequence variations of LMP1 and malignant or non-malignant isolate origin.

Keywords:
  • EBV
  • latent membrane protein 1
  • sequence variations
  • repeat elements

How to Cite

Sarshari, B., Mohebbi, S. R., Ravanshad, M., Shahrokh, S., & Zali, M. R. (2022). Sequence variations of Epstein–Barr virus LMP1 gene in gastric cancer and chronic gastritis isolates from Iranian patients: EBV LMP1 variations in GC and chronic gastritis patients. Gastroenterology and Hepatology from Bed to Bench, 15(3). https://doi.org/10.22037/ghfbb.v15i3.2578
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