ISSN: 2008-2258
Vol 15, No 1 (2022):Winter
Effect of valproic acid on SOCS-1, SOCS-2, SOCS-3, SOCS-5, SOCS6, and SOCS-7 genes expression and cell growth inhibition in colon carcinoma
Gastroenterology and Hepatology from Bed to Bench,
,
https://doi.org/10.22037/ghfbb.vi.2320
Abstract
Backgrounds: Cancer is a process induced by the accumulation of epigenetic alterations such as DNA methylation and histone deacetylation. The DNA methylation and histone deacetylation of tumor suppressor genes (TSGs) have been shown in various cancers. Recent studies have shown that silencing of TSGs, resulting from epigenetic alterations such as deacetylation, is an early event in many human cancers. The methylation and deacetylation of suppressor of cytokine signaling (SOCS) family, as TSGs, have been demonstrated in numerous cancers. Histone deacetylase inhibitors (HDACIs) have emerged as the accessory therapeutic agents for human cancers, they can inhibit the activity of specific HDACs, restore the expression of some TSGs, and induce cell growth inhibition and apoptosis. The current study aimed to investigate the effect of valproic acid (VPA) on SOCS-1, SOCS-2, SOCS-3, SOCS-5, SOCS6, and SOCS-7 gene expression and cell growth inhibition in colon carcinoma IS1, IS2, and IS3 cell lines. Materials and Methods: The IS1, IS2, and IS3 cells were cultured and treated with VPA. To determine cell viability, cell apoptosis, and the relative gene expression level, MTT assay, flow cytometry assay, and qRT-PCR were done respectively. Results: VPA changed the expression level of the SOCS-1, SOCS-2, SOCS-3, SOCS-5, SOCS6, and SOCS-7 gene by which induced cell apoptosis and inhibit cell growth in all three cell lines, IS1, IS2, and IS3. Conclusion: VPA can induce apoptosis through reactivation of SOCS-1, SOCS-2, SOCS-3, SOCS-5, SOCS6, and SOCS-7 gene expression.
- valproic acid, SOCSs, apoptosis, colon carcinoma
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References
2. A. Gery S, Komatsu N, Kawamata N, Miller CW, Desmond J, Virk RK, et al. Epigenetic silencing of the candidate tumor suppressor gene Per1 in non–small cell lung cancer. Clinical cancer research. 2007;13:1399-404.
3. Puhr M, Santer FR, Neuwirt H, Susani M, Nemeth JA, Hobisch A, et al. Down-regulation of suppressor of cytokine signaling-3 causes prostate cancer cell death through activation of the extrinsic and intrinsic apoptosis pathways. Cancer research. 2009;69: 7375-84.
4. Kim M-H, Kim M-S, Kim W, Kang MA, Cacalano NA, Kang S-B, et al. Suppressor of cytokine signaling (SOCS) genes are silenced by DNA hypermethylation and histone deacetylation and regulate response to radiotherapy in cervical cancer cells. PloS one. 2015;10: e0123133.
5. Pan LN, Lu J, Huang B. HDAC inhibitors: a potential new category of anti-tumor agents. Cell Mol Immunol. 2007;4:337-43.
6. Sanaei M, Kavoosi F, Behjoo H. EFFECT OF VALPROIC ACID AND ZEBULARINE ON SOCS-1 AND SOCS-3 GENE EXPRESSION IN COLON CARCINOMA SW 48 CELL LINE. Experimental Oncology. 2020;42:183-7.
7. Sanaei M, Kavoosi F, Roustazadeh A, Shahsavani H. In vitro effect of the histone deacetylase inhibitor valproic acid on viability and apoptosis of the PLC/PRF5 human hepatocellular carcinoma cell line. Asian Pacific journal of cancer prevention: APJCP 2018;19: 2507.
8. Sanaei M, Kavoosi F. Effect of curcumin and trichostatin a on the expression of DNA methyltransfrase 1 in hepatocellular carcinoma cell line hepa 1-6. Iranian Journal of Pediatric Hematology and Oncology 2018;8: 193-201.
9. Sanaei M, Kavoosi F. Effects of 5-aza-2ˈ-deoxycytidine and Valproic Acid on Epigenetic-modifying DNMT1 Gene Expression, Apoptosis Induction and Cell Viability in Hepatocellular Carcinoma WCH-17 cell line. Iran J Ped Hematol Oncol. 2019, 9: 83-90.
10. Sanaei M, Kavoosi F. Effect of DNA methyltransferase in comparison to and in combination with histone deacetylase inhibitors on hepatocellular carcinoma HepG2 cell line. Asian Pacific journal of cancer prevention: APJCP. 2019;20: 1119.
11. Sanaei M, Kavoosi F, Salehi H. Genistein and trichostatin a induction of estrogen receptor alpha gene expression, apoptosis and cell growth inhibition in hepatocellular carcinoma HepG 2 cells. Asian Pacific journal of cancer prevention: APJCP. 2017;18: 3445.
12. Sanaei M, Kavoosi F, Pourahmadi M, Moosavi SN. Effect of Genistein and 17-β Estradiol on the Viability and Apoptosis of Human Hepatocellular Carcinoma HepG2 cell line. Adv Biomed Res. 2017; 6: 163.
13.Masood KI, Rottenberg ME, Salahuddin N, Irfan M, Rao N, Carow B, et al. Expression of M. tuberculosis-induced suppressor of cytokine signaling (SOCS) 1, SOCS3, FoxP3 and secretion of IL-6 associates with differing clinical severity of tuberculosis. BMC infectious diseases. 2013; 13:13.
14. Leon AJ, Gomez E, Garrote JA, Bernardo D, Barrera A, Marcos JL, et al. High levels of proinflammatory cytokines, but not markers of tissue injury, in unaffected intestinal areas from patients with IBD. Mediators of inflammation. 2009;5: 1-10.
15. Zhang W, Li X, Tang Y, Chen C, Jing R, Liu T. miR-155-5p Implicates in the Pathogenesis of Renal Fibrosis via Targeting SOCS1 and SOCS6. Oxidative Medicine and Cellular Longevity. 2020;2020.
16. Ahmed Q, Manzoor S, Tariq M, Khalid M, Ashraf W, Parvaiz F, et al. Hepatitis C virus infection in vitro triggers endoplasmic reticulum stress and downregulates insulin receptor substrates 1 and 2 through upregulation of cytokine signaling suppressor 3. Acta Virol. 2014;58:238-44.
17. Banu H, Anand D, Bedekar MK, Rajendran K, Makesh M. Monoclonal antibodies against recombinant GAPDH of Edwardsiella tarda reveal the conserved nature of the protein. Food and Agricultural Immunology. 2017;28:685-98.
18. Kazanets A, Shorstova T, Hilmi K, Marques M, Witcher M. Epigenetic silencing of tumor suppressor genes: Paradigms, puzzles, and potential. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer. 2016;1865:275-88.
19. Liu K-Y, Wang L-T, Hsu S-H. Modification of epigenetic histone acetylation in hepatocellular carcinoma. Cancers. 2018;10:8.
20. Emanuele S, Lauricella M, Tesoriere G. Histone deacetylase inhibitors: apoptotic effects and clinical implications. International journal of oncology. 2008;33(4):637-46.
21. Akada H, Akada S, Gajra A, Bair A, Graziano S, Hutchison RE, et al. Efficacy of vorinostat in a murine model of polycythemia vera. Blood, The Journal of the American Society of Hematology. 2012;119:3779-89.
22. Gao S-m, Chen C-q, Wang L-y, Hong L-l, Wu J-b, Dong P-h, et al. Histone deacetylases inhibitor sodium butyrate inhibits JAK2/STAT signaling through upregulation of SOCS1 and SOCS3 mediated by HDAC8 inhibition in myeloproliferative neoplasms. Experimental hematology. 2013;41:261-70. e4.
23. Chen C-q, Yu K, Yan Q-x, Xing C-y, Chen Y, Yan Z, et al. Pure curcumin increases the expression of SOCS1 and SOCS3 in myeloproliferative neoplasms through suppressing class Ι histone deacetylases. Carcinogenesis. 2013;34:1442-9.
24. Xiong H, Du W, Zhang YJ, Hong J, Su WY, Tang JT, et al. Trichostatin A, a histone deacetylase inhibitor, suppresses JAK2/STAT3 signaling via inducing the promoter‐associated histone acetylation of SOCS1 and SOCS3 in human colorectal cancer cells. Molecular carcinogenesis. 2012;51:174-84.
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