Detection and Characterization of Rotavirus G and P Types from children with acute gastroenteritis in Qom, central Iran
Gastroenterology and Hepatology from Bed to Bench,
9 December 2020
Objective: The aim of the study is to estimate the burden of Rotavirus gastroenteritis as well as predominant genotypes of Rotavirus among children less than 5 years of age referred to Children's University Hospital in Qom, Iran.
Method: During two years, 130 patients referred to a children's hospital were enrolled in this study. After RNA extraction, Rotaviruses were detected by the VP6 gene. Then, G-typing (G1, G2, G3, G4, G8, G9, and G12) and P-typing (P4, P6, and P8) were performed using RT-PCR and specific primers.
Results: The results of the PCR showed that from a total of 130 patients, 22 cases (16.9%) showed positive VP6 by RT-PCR.G1 was mostly the predominant serotype (27%), accounting for 22% of all VP7-positive isolates, followed by G9 (18%),G2 (9%), G3 (9%), andG4 (9%). None of the strains revealed the presence of G8 genotype (0%) and 5 specimens (23%) were non-typable.
The frequency of P typing was P8 (50%), P6 (23%), P4 (14%), and3 samples were P-non-typable (13%), respectively. The dominant of G-P combination was G1  (32%).
Conclusions: Such studies base on typing methods assists in the Rotavirus vaccine introduction by policymakers and design new effective vaccines.
- Diarrhea, Rotavirus, Genotyping, acute gastroenteritis, vaccine, Iran
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