The Role of extracellular matrix proteins in gastric cancer development via epithelial-mesenchymal transition
Gastroenterology and Hepatology from Bed to Bench,
9 December 2020
Background: Gastric cancer (GC) is one of the most incident gastrointestinal cancers with high rate of mortality. Metastasis is one of the most challenging problems in gastric cancer treatment. Epithelial mesenchymal transition (EMT) of cancer cells is a complicated process controlled by different cells and molecular pathways which is considered as an important step in metastasis beginning.
Objectives: Due to finding better perception from environment of EMT, we evaluated the expression of some candidate extra cellular matrix (ECM) proteins including of THBS2, OSMR and CHI3L1 which were collected from RNA-seq bioinformatic analyses in our studies.
Materials and methods: AGS gastric cancer cell line was cultured and treated by TGF-β. EMT induction was verified by measuring expression of E-cadherin, Snail, β-catenin and Vimentin genes by real time PCR. Then, in following of our previous study, we evaluated expression of THBS2, OSMR and CHI3L1 genes in EMT induced cells by real time PCR
Results: Downregulation of E-cadherin and upregulation of Snail, β-catenin and Vimentin genes were verified in AGS treated cells in comparison with none-treated cells (P-value = 0.0355, P-value = 0.007, P-value = 0.0059, P-value = 0.0206 respectively). Also, Upregulation of THBS2, OSMR and CHI3L1 were validated in these cells after EMT induction (P-value = 0.0147, P-value = 0.05, P-value = 0.05 respectively).
Conclusion: Our morphological and molecular results validated EMT induction by TGF- β cytokine in AGS gastric cancer cell line. Furthermore, significant upregulation of candidate genes including of THBS2, OSMR and CHI3L1 verified the role of these proteins in gastric cancer invasiveness. However, further studies are needed for validation of prognostic value of these markers.
- Key words: Gastric cancer (GC), Epithelial mesenchymal transition (EMT), TGF- β cytokine, THBS2, OSMR and CHI3L1
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