ISSN: 2008-2258
Vol 13, (2020): Supplement1- Winter
Evaluation of CXCR1 as a possible diagnostic biomarker in acute appendicitis
Gastroenterology and Hepatology from Bed to Bench,
,
,
Page S106-S112
https://doi.org/10.22037/ghfbb.v13i1.1950
Abstract
Aim: The present study was conducted to determine the genes with common expression in blood and appendix tissue samples in order to introduce them as possible diagnostic biomarkers.
Background: Diagnosis of acute appendicitis (AA) without applying computed tomographytomography (CT), subjecting the patient to significant radiation, can be surprisingly difficult. Blood circulation may have conscious alterations in its RNA, protein, or metabolite composition.
Methods: The genes related to appendix tissue and blood samples of the patients with AA were extracted from public databases. Fold change (FC) ≥ 2 in blood and FC ≥ 5 in appendix tissue samples were considered to screen differentially expressed genes (DEGs). A protein-protein interaction network was organized using the search tool for retrieval of interacting genes and proteins (STRING) database as a plugin of Cytoscape software version 3.6.0. The main genes were enriched by DAVID Bioinformatics Resources to find the related biochemical pathways.
Results: Among the DEGs in blood and appendix tissue samples, C-X-C motif chemokine receptor 1(CXCR1), leukocyte immunoglobulin-like receptor A3 (LILRA3), low-affinity immunoglobulin gamma Fc region receptor III (FCGR3), and superoxide dismutase 2(SOD2) were common in both sources. CXCR1 was found as only hub gene upregulated in both blood and tissue of the patients with AA compared to controls and those with other abdominal pain.
Conclusion: CXCR1, FCGR3, LILRA3, and SOD2 were determined as a suitable possible biomarker panel for diagnosis of AA disease.
Keywords: Acute appendicitis; Biomarker, Diagnosis.
(Please cite as: Khalkhal E, Razzaghi Z, Akbarzadeh Baghban AR, Naderi N, Rezaei-Tavirani M, Rezaei-Tavirani M. Evaluation of CXCR1 as a possible diagnostic biomarker in acute appendicitis. Gastroenterol Hepatol Bed Bench 2020;13(Suppl.1):S106-S112).
- Acute Appendicitis
- biomarker
- diagnosis
How to Cite
References
Davies GM, Dasbach EJ, Teutsch S. The burden of appendicitis-related hospitalizations in the United States in 1997. Surg Infect 2004;5:160-5.
Karagulle E, Türk E, Ezer A, Nursal TZ, Kulaksızoğlu S. Value of plasma viscosity in acute appendicitis: A preliminary study. J Med Med Sci 2010;1:423,5.
Bhuiya FA. Emergency department visits for chest pain and abdominal pain: United States, 1999-2008. NCHS Data Brief 2010;43:1-8.
Buckius MT, McGrath B, Monk J, Grim R, Bell T, Ahuja VJ. Changing epidemiology of acute appendicitis in the United States: study period 1993–2008. J Surg Res 2012;175:185-90.
Poortman P, Oostvogel HJ, Bosma E, Lohle PN, Cuesta MA, de Lange-de Klerk ES, et al. Improving diagnosis of acute appendicitis: results of a diagnostic pathway with standard use of ultrasonography followed by selective use of CT. J Am Coll Surg 2009;208:434-41.
Murphy C, Glickman J, Tomczak K, Wang Y, Beggs A, Shannon M, et al. Acute appendicitis is characterized by a uniform and highly selective pattern of inflammatory gene expression. Mucosal Immunol 2008;1:297.
Brunicardi FC, Editor. Schwartz's principles of surgery. New York, NY: McGraw-Hill; 2005.
Charfi S, Sellami A, Affes A, Yaïch K, Mzali R, Boudawara TS. Histopathological findings in appendectomy specimens: a study of 24,697 cases. Int J Colorectal 2014;29:1009-12.
Drake FT, Florence MG, Johnson MG, Jurkovich GJ, Kwon S, Schmidt Z, et al. Progress in the diagnosis of appendicitis: a report from Washington State's Surgical Care and Outcomes Assessment Program. Ann Surg 2012;256:586-94.
Kirkil C, Karabulut K, Aygen E, İlhan YS, Yur M, Binnetoglu K, et al. Appendicitis scores may be useful in reducing the costs of treatment for right lower quadrant pain. Ulus Travma Acil Cerrahi Derg 2013;19:13-9.
Ge Q, Chen L, Tang M, Zhang S, Liu L, Gao L, et al. Analysis of mulberry leaf components in the treatment of diabetes using network pharmacology. Eur Pharmacol 2018;833:50-62.
Chawla LS, Toma I, Davison D, Vaziri K, Lee J, Lucas R, et al. Acute appendicitis: transcript profiling of blood identifies promising biomarkers and potential underlying processes. BMC Med Genomics 2016;9:40.
Goudarzi M, Goudarzi H, Alebouyeh M, Azimi Rad M, Shayegan Mehr FS, Zali MR, et al. Antimicrobial susceptibility of clostridium difficile clinical isolates in iran. Iran Red Crescent Med J 2013;15:704-11.
Farnood A, Naderi N, Moghaddam SJ, Noorinayer B, Firouzi F, Aghazadeh R, et al. The frequency of C3435T MDR1 gene polymorphism in Iranian patients with ulcerative colitis. Int J Colorectal Dis 2007;22:999-1003.
Sivit CJ, Siegel MJ, Applegate KE, Newman KD. When appendicitis is suspected in children. Radiographics 2001;21:247-62.
Memon ZA, Irfan S, Fatima K, Iqbal MS, Sami W. Acute appendicitis: diagnostic accuracy of Alvarado scoring system. Asian J Surg 2013;36:144-9.
Teixeira P, Demetriades D. Appendicitis: changing perspectives. Adv Surg 2013;47:119-40.
Collins GB, Tan TJ, Gifford J, Tan A. The accuracy of pre-appendectomy computed tomography with histopathological correlation: a clinical audit, case discussion and evaluation of the literature. Emerg Radiol 2014;21:589-95.
Rosen MP, Ding A, Blake MA, Baker ME, Cash BD, Fidler JL, et al. ACR Appropriateness Criteria® right lower quadrant pain—suspected appendicitis. J Am Coll Radiol 2011;8:749-55.
Ahn SJT. LOCAT (low-dose computed tomography for appendicitis trial) comparing clinical outcomes following low-vs standard-dose computed tomography as the first-line imaging test in adolescents and young adults with suspected acute appendicitis: study protocol for a randomized controlled trial. Trial 2014;15:28.
Miglioretti DL, Johnson E, Williams A, Greenlee RT, Weinmann S, Solberg LI, et al. The use of computed tomography in pediatrics and the associated radiation exposure and estimated cancer risk. JAMA Pediatr 2013;167:700-7.
Wai S, Ma L, Kim E, Adekunle-Ojo A. The utility of the emergency department observation unit for children with abdominal pain. Pediatr Emegr Care 2013;29:574-8.
Godaly G, Hang L, Frendéus B, Svanborg C. Transepithelial neutrophil migration is CXCR1 dependent in vitro and is defective in IL-8 receptor knockout mice. J Immunol 2000;165:5287-94.
Hang L, Frendéus B, Godaly G, Svanborg C. Interleukin-8 receptor knockout mice have subepithelial neutrophil entrapment and renal scarring following acute pyelonephritis. J Infect Dis 2000;182:1738-48.
Dalal I, Somekh E, Bilker-Reich A, Boaz M, Gorenstein A, Serour F. Serum and peritoneal inflammatory mediators in children with suspected acute appendicitis. Arch Surg 2005;140:169-73.
Zeillemaker AM, van Papendrecht AAH, Hart MH, Roos D, Verbrugh HA, Leguit P. Peritoneal interleukin-8 in acute appendicitis. J Surg Res 1996;62:273-7.
Yoon DY, Chu J, Chandler C, Hiyama S. Human cytokine levels in nonperforated versus perforated appendicitis: molecular serum markers for extent of disease? Am Surg 2002;68:1033.
Vivier E, Tomasello E, Baratin M, Walzer T, Ugolini S. Functions of natural killer cells. Nat Immunol 2008;9:503.
Zhang Y, Boesen CC, Radaev S, Brooks AG, Fridman WH, Sautes-Fridman C, et al. Crystal structure of the extracellular domain of a human FcγRIII. Immunity 2000;13:387-95.
Arm JP, Nwankwo C, Austen KF. Molecular identification of a novel family of human Ig superfamily members that possess immunoreceptor tyrosine-based inhibition motifs and homology to the mouse gp49B1 inhibitory receptor. J Immunol 1997;159:2342-9.
Tedla N, An H, Fath T, Lord M, Bryant K. Leukocyte immunoglobulin-like receptor A3 (LILRA3) displays anti-inflammatory and neuro-regenerative functions through interaction with multiple ligands. J Immunol 2017;198(1 Supplement). 221.10. [Abstract]
- Abstract Viewed: 0 times
- PDF Downloaded: 0 times
