Evaluation of CXCR1 as a Possible Diagnostic Biomarker in Acute Appendicitis
Gastroenterology and Hepatology from Bed to Bench,
9 December 2020
Background: Diagnosis of acute appendicitis (AA) without computed tomography, subject to significant radiation, can be surprisingly difficult. Blood circulation may have conscious alterations in its RNA, protein, or metabolite composition.
AIM: The aim of this study was to obtain genes with commonly expression in blood and appendix tissue samples to introduce as possible diagnostic biomarkers.
Methods: The genes related to tissue and blood of AA patients were extracted from public databases. Fold change (FC) ? 2 in blood and FC ? 5 in appendix tissue samples were considered to screen the differential expressed genes (DEGs). a protein-protein interaction network was organized with using of STRING database as a plugin of Cytoscape software version 3.6.0. The main genes were enriched by DAVID Bioinformatics Resources to find the related biochemical pathways.
Results: Among the differential expressed genes in blood and appendix tissue samples, CXCR1, LILRA3, FCGR3, and SOD2 are common in both sources. CXCR1 is only hub gene that is up regulated in both blood and tissue of patients compared to controls and other abdominal pain.
Conclusion: CXCR1 beside FCGR3, LILRA3, and SOD2 is a suitable possible biomarker panel to diagnose AA disease.
- Acute Appendicitis
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