ISSN: 2008-2258
Vol 14, No 1 (2021): Winter
The frequency of HLA-DQ2/DQ8 haplotypes and celiac disease among the first-degree relatives of patients with celiac disease
Gastroenterology and Hepatology from Bed to Bench,
,
,
Page 36-43
https://doi.org/10.22037/ghfbb.v14i1.1922
Abstract
Aim: To evaluate the frequency of human leukocyte antigen (HLA) DQ2/DQ8 haplotyp
Aim: We evaluated the frequency of human leukocyte antigen (HLA) DQ2/DQ8 haplotypes as well as celiac disease (CD) among the first-degree relatives (FDRs) of CD patients, compared with healthy controls, and compared the HLA typing with serologic tests in this population.
Background: Until now, no study has examined the frequency of HLA-DQ2/DQ8 haplotypes among the FDRs of Iranian patients with CD.
Methods: In the current case-control study, 100 FDRs of CD patients and 151 healthy controls were included. Demographic characteristics were assessed using a research-made questionnaire. A blood sample was collected from each participant for HLA-DQ typing and measuring serum levels of anti-gliadin and anti-transglutaminase (anti-tTG) antibodies.
Results: The mean age of the FDRs of CD patients and controls was 30 and 35 years, respectively. Also, 51% (n=51) of the FDRs and 51.7% (n=78) of controls were female. CD was diagnosed among 3% (n=3) of the FDRs of CD patients. No significant difference was found in terms of the frequency of HLA-DQ alleles between the FDRs of CD patients and controls. Out of 100 FDRs of CD patients, 40% had HLA-DQ2 allele, 16% carried HLA-DQ8 allele, and 4% had both alleles. Surprisingly, the CD was diagnosed in three subjects among 60 FDRs of CD patients with HLA-DQ2 allele (3% of the whole population). This diagnosis was based on the results of serological tests as well as endoscopy and intestinal biopsy.
Conclusion: CD was confirmed among 3% (n=3) of the FDRs of CD patients. We found that HLA typing is not effective in predicting CD among FDRs of CD patients. Other methods such as serological tests have a higher priority compared with HLA-DQ typing.
Keywords: Celiac disease, HLA-DQ2, HLA-DQ8, HLA typing, Iran.
(Please cite as: Mansouri M, Dadfar M, Rostami-Nejad M, Ekhlasi GH, Shahbazkhani AH, Shahbazkhani B. The frequency of HLA-DQ2/DQ8 haplotypes and celiac disease among the first-degree relatives of patients with celiac disease. Gastroenterol Hepatol Bed Bench 2021;14(1):36-43).
es as well as celiac disease (CD) among the first-degree relatives (FDRs) of CD patients, compared with healthy controls, and to compare the HLA typing with serologic tests in this population.
Background: Until now, no study has examined the frequency of HLA-DQ2/DQ8 haplotypes among the FDRs of Iranian patients with CD.
Methods: In the current case-control study, 100 FDRs of CD patients and 151 healthy controls were included. Demographic characteristics were assessed using a research-made questionnaire. A blood sample was collected from each participant for HLA-DQ typing and measuring serum levels of anti-gliadin and anti-transglutaminase (anti-tTG) antibodies.
Results: Mean age of the FDRs of CD patients and controls was 30 and 35 years, respectively. Also, 51% (n=51) of the FDRs and 51.7% (n=78) of controls were female. CD was diagnosed among 3% (n=3) of the FDRs of CD patients. No significant difference was found in terms of the frequency of HLA-DQ alleles between the FDRs of CD patients and controls. Out of 100 FDRs of CD patients, 40% had HLA-DQ2 allele, 16% carried HLA-DQ8 allele, and 4% had both alleles. Surprisingly, the CD was diagnosed in three subjects among 60 FDRs of CD patients with HLA-DQ2 allele (3% of the whole population). This diagnosis was based on the results of serological tests as well as endoscopy and intestinal biopsy.
Conclusion: CD was confirmed among 3% (n=3) of the FDRs of CD patients. We found that HLA typing is not effective in predicting CD among FDRs of CD patients. Other methods such as serological tests have a higher priority compared with HLA-DQ typing.
KEYWORDS: Celiac disease, HLA-DQ2, HLA-DQ8, HLA typing, Iran
- Celiac disease
- HLA DQ2
- HLA DQ8
- HLA typing
- Iran
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References
Ludvigsson JF, Leffler DA, Bai JC, Biagi F, Fasano A, Green PH, et al. The Oslo definitions for coeliac disease and related terms. Gut 2013;62:43-52.
Green PH, Cellier C. Celiac disease. N Engl J Med. 2007;357:1731-43.
Al-Hussaini A, Eltayeb-Elsheikh N, Alharthi H, Osman A, Alshahrani M, Sandogji I, et al. HLA-DQ genotypes relative risks for celiac disease in Arabs: A case-control study. J Dig Dis 2019;20:602-8.
Khosravi A, Mansouri M, Rostami-Nejad M, Shahbazkhani B, Ekhlasi G, Kalantari E. The likelihood ratio and frequency of DQ2/DQ8 haplotypes in Iranian patients with celiac disease. Gastroenterol Hepatol Bed Bench 2016;9:18-24.
Tack GJ, Verbeek WH, Schreurs MW, Mulder CJ. The spectrum of celiac disease: epidemiology, clinical aspects and treatment. Nat Rev Gastroenterol Hepatol 2010;7:204-13.
6. Kagnoff MF. Celiac disease: pathogenesis of a model immunogenetic disease. J Clin Invest 2007;117:41-9.
Majsiak E, Cichoz-Lach H, Gubska O, Cukrowska B. [Celiac disease - disease of children and adults: symptoms, disease complications, risk groups and comorbidities]. Pol Merkur Lekarski 2018;44:31-5.
Verma AK, Gatti S, Lionetti E, Galeazzi T, Monachesi C, Franceschini E, et al. Comparison of Diagnostic Performance of the IgA Anti-tTG Test vs IgA Anti-Native Gliadin Antibodies Test in Detection of Celiac Disease in the General Population. Clin Gastroenterol Hepatol 2018;16:1997-8.
Martinez-Ojinaga E, Molina M, Polanco I, Urcelay E, Nunez C. HLA-DQ distribution and risk assessment of celiac disease in a Spanish center. Rev Esp Enferm Dig 2018;110:421-6.
Martinez-Ojinaga E, Fernandez-Prieto M, Molina M, Polanco I, Urcelay E, Nunez C. Influence of HLA on clinical and analytical features of pediatric celiac disease. BMC Gastroenterol 2019;19:91.
Ozgenel SM, Temel T, Uskudar Teke H, Yildiz P, Korkmaz H, Ozakyol A. HLA-DQ2/DQ8 frequency in adult patients with celiac disease, their first-degree relatives, and normal population in Turkey. Turk J Gastroenterol 2019;30:321-5.
Megiorni F, Pizzuti A. HLA-DQA1 and HLA-DQB1 in Celiac disease predisposition: practical implications of the HLA molecular typing. J Biomed Sci 2012;19:88.
Farina F, Picascia S, Pisapia L, Barba P, Vitale S, Franzese A, et al. HLA-DQA1 and HLA-DQB1 Alleles, Conferring Susceptibility to Celiac Disease and Type 1 Diabetes, are More Expressed Than Non-Predisposing Alleles and are Coordinately Regulated. Cells 2019;8.
Kaukinen K, Partanen J, Maki M, Collin P. HLA-DQ typing in the diagnosis of celiac disease. Am J Gastroenterol 2002;97:695-9.
Piancatelli D, Ben El Barhdadi I, Oumhani K, Sebastiani P, Colanardi A, Essaid A. HLA Typing and Celiac Disease in Moroccans. Med Sci 2017;5.
Humphry RW, Cameron A, Gunn GJ. A practical approach to calculate sample size for herd prevalence surveys. PrevVeter Med 2004;65:173-88.
Lopes LHC, Muniz JG, Oliveira RP, Sdepanian VL. Celiac Disease in Brazilian First-degree Relatives: The Odds Are Five Times Greater for HLA DQ2 Homozygous. J Pediatr Gastroenterol Nutr 2019;68:e77-e80.
DiGiacomo D, Santonicola A, Zingone F, Troncone E, Caria MC, Borgheresi P, et al. Human leukocyte antigen DQ2/8 prevalence in non-celiac patients with gastrointestinal diseases. World J Gastroenterol 2013;19:2507-13.
19. Ortiz G, Messere G, Toca MDC, Fiorucci M, Bigliardi R, Vidal J, et al. IgA anti-tissue transglutaminase antibodies and IgG antibodies against deamidated gliadin peptides as predictors of celiac disease. Arch Argent Pediatr 2019;117:52-5.
Castellana M, Castellana C, Giovanella L, Trimboli P. Prevalence of gastrointestinal disorders having an impact on tablet levothyroxine absorption: should this formulation still be considered as the first-line therapy? Endocrine 2020.
Chomeili B, Aminzadeh M, Hardani AK, Fathizadeh P, Chomeili P, Azaran A. Prevalence of celiac disease in siblings of Iranian patients with celiac disease. Arquivos Gastroenterologia 2011;48:131-5.
Lebwohl B, Sanders DS, Green PHR. Coeliac disease. Lancet 2018;391:70-81.
Milajerdi A, Sadeghi O, Siadat SD, Keshavarz SA, Sima A, Vahedi H, et al. A randomized controlled trial investigating the effect of a diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols on the intestinal microbiome and inflammation in patients with ulcerative colitis: study protocol for a randomized controlled trial. Trials 2020;21:201.
Sadeghi O, Milajerdi A, Siadat SD, Keshavarz SA, Sima AR, Vahedi H, et al. Effects of soy milk consumption on gut microbiota, inflammatory markers, and disease severity in patients with ulcerative colitis: a study protocol for a randomized clinical trial. Trials 2020;21:565.
Saneei P, Sadeghi O, Feizi A, Keshteli AH, Daghaghzadeh H, Esmaillzadeh A, et al. Relationship between spicy food intake and chronic uninvestigated dyspepsia in Iranian adults. J Dig Dis 2016;17:28-35.
Choung RS, Mills JR, Snyder MR, Murray JA, Gandhi MJ. Celiac disease risk stratification based on HLA-DQ heterodimer (HLA-DQA1 ~ DQB1) typing in a large cohort of adults with suspected celiac disease. Hum Immunol 2020.
Vaquero L, Caminero A, Nunez A, Hernando M, Iglesias C, Casqueiro J, et al. Coeliac disease screening in first-degree relatives on the basis of biopsy and genetic risk. Eur J Gastroenterol Hepatol 2014;26:263-7.
Cecilio LA, Bonatto MW. The prevalence of HLA DQ2 and DQ8 in patients with celiac disease, in family and in general population. Arq Bras Cir Dig 2015;28:183-5.
Mishra A, Prakash S, Kaur G, Sreenivas V, Ahuja V, Gupta SD, et al. Prevalence of celiac disease among first-degree relatives of Indian celiac disease patients. Dig Liver Dis 2016;48:255-9.
Bahari A, Izadi S, Karimi M, Moghadam ES, Bari Z, Esmaeilzadeh A, et al. HLA-DQ2 and HLA-DQ8 Genotyping in a Sample of Iranian Celiac Patients and Their First-Degree Relatives. Govaresh 2014;18:242-5.
Wessels MMS, de Rooij N, Roovers L, Verhage J, de Vries W, Mearin ML. Towards an individual screening strategy for first-degree relatives of celiac patients. Eur J Pediatr 2018;177:1585-92.
Vaquero L, Rodriguez-Martin L, Alvarez-Cuenllas B, Hernando M, Iglesias-Blazquez C, Menendez-Arias C, et al. Coeliac disease and gastrointestinal symptom screening in adult first-degree relatives. J Gastroenterol Hepatol 2017;32:1931-7.
Rostami-Nejad M, Romanos J, Rostami K, Ganji A, Ehsani-Ardakani MJ, Bakhshipour AR, et al. Allele and haplotype frequencies for HLA-DQ in Iranian celiac disease patients. World J Gastroenterol 2014;20:6302-8.
Llorente-Alonso MJ, Fernandez-Acenero MJ, Sebastian M. Gluten intolerance: sex and age-related features. Can J Gastroenterol 2006;20:719-22.
Megiorni F, Mora B, Bonamico M, Barbato M, Montuori M, Viola F, et al. HLA-DQ and susceptibility to celiac disease: evidence for gender differences and parent-of-origin effects. Am J Gastroenterol 2008;103:997-1003.
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