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  3. Vol 14, No 1 (2021): Winter
  4. Original Article

ISSN: 2008-2258

Vol 14, No 1 (2021): Winter

Increased regulatory T cells in peripheral blood of children with eosinophilic esophagitis

  • Mahnaz Abdolahi
  • Shima Rasouli
  • Delara Babaie
  • Naghi Dara
  • Farid Imanzadeh
  • Aliakbar Sayyari
  • Pejman Rouhani
  • Katayoun Khatami
  • Maryam Kazemiaghdam
  • Yalda Nilipour
  • Maliheh Khoddami
  • Farah Ghadimi
  • Fatemeh Mousavinasab
  • Mehrnaz Mesdaghi

Gastroenterology and Hepatology from Bed to Bench, , , Page 25-30
https://doi.org/10.22037/ghfbb.v14i1.1879 Published 18 January 2021

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Abstract

Aim: Considering the allergic basis of Eosinophilic esophagitis (EoE), this study was conducted to evaluate peripheral blood Tregs in children with EoE.


Background: Eosinophilic esophagitis (EoE) is an allergic inflammatory disease of gastrointestinal tract. Regulatory T cells (Tregs) have a confirmed role in allergic disorders.


Methods: Children with EoE, gastroesophageal reflux disease (GERD) and healthy controls (HC) (10 subjects in each group) were recruited after diagnosis by a pediatric gastroenterologist and allergist. After obtaining informed written consent, peripheral blood was obtained. Peripheral blood mononuclear cells were isolated by Ficoll gradient centrifugation. Flowcytometry was used to enumerate peripheral blood Tregs (CD4+CD25+FOXP3+ gated lymphocytes were considered as Tregs).


Results: CD4+ gated lymphocytes significantly increased in EoE and GERD groups compared to HC group (p= 0.018). Tregs also was significantly increased in EoE in comparison to HC group (p=0.016). There were no statistically significant differences in Tregs of EoE as compared to GERD subjects (p=0.085).


Conclusion: Peripheral blood Tregs increase in patients with EoE as compared to healthy controls, which may be indicative of a feedback mechanism to regulate inflammatory responses.


Keywords: Regulatory T cells, Eosinophilic esophagitis, Gastroesophageal reflux disease, Peripheral blood mononuclear cells.


(Please cite as: Abdolahy M, Rasouli SH, Babaie D, Dara N, Imanzadeh F, Sayyari AA, et al. Increased regulatory T cells in peripheral blood of children with eosinophilic esophagitis. Gastroenterol Hepatol Bed Bench 2021;14(1):25-30).

Keywords:
  • Key words
  • Regulatory T Cells
  • Eosinophilic Esophagitis (EoE)
  • Gastroesophageal Reflux Disease (GERD)
  • Peripheral Blood Mononuclear Cells (PBMC).
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How to Cite

Abdolahi, M., Rasouli, S., Babaie, D., Dara, N., Imanzadeh, F., Sayyari, A., Rouhani, P., Khatami, K., Kazemiaghdam, M., Nilipour, Y., Khoddami, M., Ghadimi, F., Mousavinasab, F., & Mesdaghi, M. (2021). Increased regulatory T cells in peripheral blood of children with eosinophilic esophagitis. Gastroenterology and Hepatology from Bed to Bench, 14(1), 25-30. https://doi.org/10.22037/ghfbb.v14i1.1879
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References

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Print ISSN: 2008-2258
Online ISSN: 2008-4234

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