Increased Regulatory T cells in Peripheral Blood of Children with Eosinophilic Esophagitis
Gastroenterology and Hepatology from Bed to Bench,
26 January 2021
Background: Eosinophilic esophagitis (EoE) is an allergic inflammatory disease of gastrointestinal tract. Regulatory T cells (Tregs) have a confirmed regulatory role in allergic disorders. Considering the allergic basis of EoE, this study was done to evaluate peripheral blood Tregs in children with EoE.
Materials & Methods: Children with EoE, gastroesophageal reflux disease (GERD) and healthy controls (HC) (10 subjects in each group) were recruited after diagnosis by a pediatric gastroenterologist and allergist. After obtaining informed written consent, peripheral blood was obtained. Peripheral blood mononuclear cells were isolated by Ficoll gradient centrifugation. Flowcytometry was used to enumerate peripheral blood Tregs and CD4+CD25+FOXP3+ cells were considered as Tregs.
Results: The percentage of CD4+ lymphocytes was significantly higher in EoE and GERD groups compared to HC group (p= 0.018). The percentage of CD4+CD25+FOXP3+ Tregs was significantly higher in EoE comparing HC group (P=0.016). The higher percentage of Tregs in EoE comparing GERD group was not statistically significant.
Conlusion: Peripheral blood Tregs are increased in patients with EoE comparing healthy controls, which may be indicative of a feedback mechanism to regulate inflammatory responses.
- Key words
- Regulatory T Cells
- Eosinophilic Esophagitis (EoE)
- Gastroesophageal Reflux Disease (GERD)
- Peripheral Blood Mononuclear Cells (PBMC).
Hruz PL, Straumann A. 44 - Eosinophil-associated gastrointestinal disorders. In: Rich RR, Fleisher TA, Shearer WT, Schroeder HW, Frew AJ, Weyand CM, editors. Clinical Immunology (Fourth Edition). London: Content Repository Only!; 2013. p. 550-7.
Dellon ES, Erichsen R, Baron JA, Shaheen NJ, Vyberg M, Sorensen HT, et al. The increasing incidence and prevalence of eosinophilic oesophagitis outpaces changes in endoscopic and biopsy practice: national population-based estimates from Denmark. Aliment Pharmacol Ther. 2015 Apr;41(7):662-70. PubMed PMID: 25684441. Pubmed Central PMCID: 4504237.
Sogut A, Yilmaz O, Kirmaz C, Ozbilgin K, Onur E, Celik O, et al. Regulatory-T, T-helper 1, and T-helper 2 cell differentiation in nasal mucosa of allergic rhinitis with olive pollen sensitivity. Int Arch Allergy Immunol. 2012;157(4):349-53. PubMed PMID: 22123238.
Zhang H, Kong H, Zeng X, Guo L, Sun X, He S. Subsets of regulatory T cells and their roles in allergy. J Transl Med. 2014 May 12;12:125. PubMed PMID: 24886492. Pubmed Central PMCID: 4023533.
Rich RR. Clinical immunology : principles and practice. 4th ed. ed. [Oxford?]: Elsevier; 2013.
Rothenberg ME, Spergel JM, Sherrill JD, Annaiah K, Martin LJ, Cianferoni A, et al. Common variants at 5q22 associate with pediatric eosinophilic esophagitis. Nat Genet. 2010 Apr;42(4):289-91. PubMed PMID: 20208534. Pubmed Central PMCID: 3740732.
Fuentebella J, Patel A, Nguyen T, Sanjanwala B, Berquist W, Kerner JA, et al. Increased number of regulatory T cells in children with eosinophilic esophagitis. J Pediatr Gastroenterol Nutr. 2010 Sep;51(3):283-9. PubMed PMID: 20639775.
Tantibhaedhyangkul U, Tatevian N, Gilger MA, Major AM, Davis CM. Increased esophageal regulatory T cells and eosinophil characteristics in children with eosinophilic esophagitis and gastroesophageal reflux disease. Ann Clin Lab Sci. 2009 Spring;39(2):99-107. PubMed PMID: 19429794.
Mousavinasab F, Babaie D, Nilipour Y, Mansouri M, Imanzadeh F, Dara N, et al. Increased number of regulatory T cells in esophageal tissue of patients with eosinophilic esophagitis in comparison to gastro esophageal reflux disease and control groups. Allergol Immunopathol (Madr). 2019 Jun 6. PubMed PMID: 31178311. Epub 2019/06/11. eng.
Rothenberg ME. 68 - Eosinophilic Gastrointestinal Disorders. In: Adkinson NF, Bochner BS, Burks AW, Busse WW, Holgate ST, Lemanske RF, et al., editors. Middleton's Allergy (Eighth Edition). London: Content Repository Only!; 2014. p. 1095-106.
Sperry SL, Woosley JT, Shaheen NJ, Dellon ES. Influence of race and gender on the presentation of eosinophilic esophagitis. The American journal of gastroenterology. 2012;107(2):215-21. PubMed PMID: 21971538. Epub 10/04. eng.
Kottyan LC, Maddox A, Hoffman K, Ray L, Forney C, Eby M, et al. X chromosomal linkage to eosinophilic esophagitis susceptibility. Journal of Allergy and Clinical Immunology. 2018;141(2):AB225.
Asanuma K, Iijima K, Shimosegawa T. Gender difference in gastro-esophageal reflux diseases. World journal of gastroenterology. 2016;22(5):1800-10. PubMed PMID: 26855539. eng.
Holgate ST. Chapter 96 - Asthma: Clinical Aspects and Mucosal Immunology. In: Mestecky J, Strober W, Russell MW, Kelsall BL, Cheroutre H, Lambrecht BN, editors. Mucosal Immunology (Fourth Edition). Boston: Academic Press; 2015. p. 1833-56.
Chen HH, Sun AH, Ojcius DM, Hu WL, Ge YM, Lin X, et al. Eosinophils from Murine Lamina Propria Induce Differentiation of Naive T Cells into Regulatory T Cells via TGF-beta1 and Retinoic Acid. PLoS One. 2015;10(11):e0142881. PubMed PMID: 26587591. Pubmed Central PMCID: PMC4654556. Epub 2015/11/21. eng.
Babaei D, Jafarian S, Chavoshzadeh Z, Mansouri M, Sayyari AA, Imanzadeh F, et al. The Study of the Serum Levels of Interleukin-10 and Tumor Growth Factor-β1 in Children With Eosinophilic Gastrointestinal Disorders Compared to Children With Gastroesophageal Reflux Disease and Healthy Controls. Immunoregulation. 2019;1(4). In Press.
Stuck MC, Straumann A, Simon HU. Relative lack of T regulatory cells in adult eosinophilic esophagitis - no normalization after corticosteroid therapy. Allergy. 2011 May;66(5):705-7. PubMed PMID: 21470243. Epub 2011/04/08. eng.
Davis BP, Rothenberg ME. Mechanisms of Disease of Eosinophilic Esophagitis. Annual review of pathology. 2016;11:365-93. PubMed PMID: 26925500. Epub 02/24. eng.
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