ISSN: 2008-2258
Vol 14, No 1 (2021): Winter
Increased regulatory T cells in peripheral blood of children with eosinophilic esophagitis
Gastroenterology and Hepatology from Bed to Bench,
,
,
Page 25-30
https://doi.org/10.22037/ghfbb.v14i1.1879
Abstract
Aim: Considering the allergic basis of Eosinophilic esophagitis (EoE), this study was conducted to evaluate peripheral blood Tregs in children with EoE.
Background: Eosinophilic esophagitis (EoE) is an allergic inflammatory disease of gastrointestinal tract. Regulatory T cells (Tregs) have a confirmed role in allergic disorders.
Methods: Children with EoE, gastroesophageal reflux disease (GERD) and healthy controls (HC) (10 subjects in each group) were recruited after diagnosis by a pediatric gastroenterologist and allergist. After obtaining informed written consent, peripheral blood was obtained. Peripheral blood mononuclear cells were isolated by Ficoll gradient centrifugation. Flowcytometry was used to enumerate peripheral blood Tregs (CD4+CD25+FOXP3+ gated lymphocytes were considered as Tregs).
Results: CD4+ gated lymphocytes significantly increased in EoE and GERD groups compared to HC group (p= 0.018). Tregs also was significantly increased in EoE in comparison to HC group (p=0.016). There were no statistically significant differences in Tregs of EoE as compared to GERD subjects (p=0.085).
Conclusion: Peripheral blood Tregs increase in patients with EoE as compared to healthy controls, which may be indicative of a feedback mechanism to regulate inflammatory responses.
Keywords: Regulatory T cells, Eosinophilic esophagitis, Gastroesophageal reflux disease, Peripheral blood mononuclear cells.
(Please cite as: Abdolahy M, Rasouli SH, Babaie D, Dara N, Imanzadeh F, Sayyari AA, et al. Increased regulatory T cells in peripheral blood of children with eosinophilic esophagitis. Gastroenterol Hepatol Bed Bench 2021;14(1):25-30).
- Key words
- Regulatory T Cells
- Eosinophilic Esophagitis (EoE)
- Gastroesophageal Reflux Disease (GERD)
- Peripheral Blood Mononuclear Cells (PBMC).
How to Cite
References
Hruz PL, Straumann A. Eosinophil-associated gastrointestinal disorders. In: Rich RR, Fleisher TA, Shearer WT, Schroeder HW, Frew AJ, Weyand CM, editors. Clinical Immunology. 4th Edition. London: Content Rep Only 2013:550-7.
Dellon ES, Erichsen R, Baron JA, Shaheen NJ, Vyberg M, Sorensen HT, et al. The increasing incidence and prevalence of eosinophilic oesophagitis outpaces changes in endoscopic and biopsy practice: national population-based estimates from Denmark. Aliment Pharmacol Ther 2015;41:662-70.
Sogut A, Yilmaz O, Kirmaz C, Ozbilgin K, Onur E, Celik O, et al. Regulatory-T, T-helper 1, and T-helper 2 cell differentiation in nasal mucosa of allergic rhinitis with olive pollen sensitivity. Int Arch Allergy Immunol 2012;157:349-53.
Zhang H, Kong H, Zeng X, Guo L, Sun X, He S. Subsets of regulatory T cells and their roles in allergy. J Transl Med 2014;12:125.
Rich RR. Clinical immunology: principles and practice. 4th edition. Oxford: Elsevier, 2013.
Rothenberg ME, Spergel JM, Sherrill JD, Annaiah K, Martin LJ, Cianferoni A, et al. Common variants at 5q22 associate with pediatric eosinophilic esophagitis. Nat Genet 2010;42:289-91.
Fuentebella J, Patel A, Nguyen T, Sanjanwala B, Berquist W, Kerner JA, et al. Increased number of regulatory T cells in children with eosinophilic esophagitis. J Pediatr Gastroenterol Nutr 2010;51:283-9.
Tantibhaedhyangkul U, Tatevian N, Gilger MA, Major AM, Davis CM. Increased esophageal regulatory T cells and eosinophil characteristics in children with eosinophilic esophagitis and gastroesophageal reflux disease. Ann Clin Lab Sci 2009;39:99-107.
Mousavinasab F, Babaie D, Nilipour Y, Mansouri M, Imanzadeh F, Dara N, et al. Increased number of regulatory T cells in esophageal tissue of patients with eosinophilic esophagitis in comparison to gastro esophageal reflux disease and control groups. Allergol Immunopathol 2019;47:431-436.
Babaie D, Daneshmandi Z, Jafarian S, Chavoshzadeh Z, Rsouli S, Mansouri M, et al. Serum Levels of Interleukin-10 and Tumor Growth Factor-β1 in Children With Eosinophilic Gastrointestinal Disorders Compared to Control Groups. Immunoregulation 2020;2:103-10.
Adkinson NF, Bochner BS, Burks AW, Busse WW, Holgate ST, Lemanske RF, et al. Middleton's Allergy Principles and Practice. 8th Edition. Content Rep Only 2014:1095-106.
Sperry SL, Woosley JT, Shaheen NJ, Dellon ES. Influence of race and gender on the presentation of eosinophilic esophagitis. Am J Gastroenterol 2012;107:215-21.
Kottyan LC, Maddox A, Hoffman K, Ray L, Forney C, Eby M, et al. X chromosomal linkage to eosinophilic esophagitis susceptibility. Journal of Allergy and Clinical Immunology 2018;141:AB225.
Asanuma K, Iijima K, Shimosegawa T. Gender difference in gastro-esophageal reflux diseases. World J Gastroenterol 2016;22:1800-10.
Holgate ST. Asthma: Clinical Aspects and Mucosal Immunology. In: Mestecky J, Strober W, Russell MW, Kelsall BL, Cheroutre H, Lambrecht BN, editors. Mucosal Immunology. 4th Edition. Boston, Cambridge: Academic Press; 2015. P.1833-56.
Chen HH, Sun AH, Ojcius DM, Hu WL, Ge YM, Lin X, et al. Eosinophils from Murine Lamina Propria Induce Differentiation of Naive T Cells into Regulatory T Cells via TGF-beta1 and Retinoic Acid. PLoS One 2015;10:e0142881.
Babaie D, Rasouli S, Darougar S, Daneshmandii Z, Mesdaghi M, Ghadimi F. Serum interleukin-17 evaluation in patients with eosinophilic gastrointestinal disease. Immunoregulation 2020;3:61-6.
Stuck MC, Straumann A, Simon HU. Relative lack of T regulatory cells in adult eosinophilic esophagitis - no normalization after corticosteroid therapy. Allergy 2011;66:705-7.
Davis BP, Rothenberg ME. Mechanisms of Disease of Eosinophilic Esophagitis. Ann Rev Pathol 2016;11:365-93.
- Abstract Viewed: 0 times
- PDF Downloaded: 0 times
