The antidiabetic and hepatoprotective effects of myricitrin on aged mice with D-galactose
Gastroenterology and Hepatology from Bed to Bench,
Vol. 13 No. 3 (2020),
2 June 2020
,
Page 247-253
https://doi.org/10.22037/ghfbb.v13i3.1782
Abstract
Aim: The present study aims to evaluate the effects of antidiabetic and hepatoprotective of myricitrin in the aged mice induced by D-galactose (D-gal).
Background: Aging occurs during a person’s life; there has been no way to stop the aging process, but antioxidant and changing lifestyles can delay it.
Methods: In this experimental study, 72 female adult mice (weighing30–35g) were randomly divided into six groups: 1: control, 2: D-gal at 500mg/kg/d, 3-5: D-gal+ Myricitrin at 5, 10 and 20mg/kg/d 6: D-gal+ Vitamin E at 100mg/kg/d. Aging induced by D-gal for 45 days via intraperitoneal. Myricitrin and Vitamin E administrated orally by gavage for the last 28 days. The blood glucose, insulin level, β-cell function, insulin resistance, hepatic enzymes, lipid profile, and histology of the liver, and pancreas were evaluated.
Results: D-gal injection increased the glucose (p<0.001) and insulin levels (p<0.01) compared to control group. Myricitrin (p<0.01) and Vitamin E (p<0.001) increased insulin and decreased blood glucose levels compared to D-gal group. Myricitrin had a similar impact on insulin levels to vitamin E. Insulin resistance induced in the D-gal group (p<0.001). Myricitrin reduced insulin resistance and increased β-cell function (p<0.01) compared to D-gal group. D-gal elevated (p<0.01) cholesterol, LDL and triglyceride level, myricitrin (p<0.001), and Vitamin E (p<0.05) were reduced.
Conclusion: D-gal-induced aging causes the accumulation of RBCs, inflammation in the liver, and changes in the number and diameter of Langerhans islets in the pancreas. Myricitrin improved these D-gal effects. Myricitrin had the anti-diabetic and hepatoprotective effects on the aged mice induced by D-galactose.
Keywords: Myricitrin, Liver, Pancreas, D-galactose, Aging.
(Please cite as: Omidi M, Ahangarpour A, Khorsandi LS, Ramezani- AliAkbari F. The antidiabetic and hepatoprotective effects of myricitrin on aged mice with D-galactose Gastroenterol Hepatol Bed Bench 2020;13(3):247-253).
- Myricitrin
- Liver
- Pancreas
- D-galactose
- Aging
How to Cite
References
Amarya S, Singh K, Sabharwal M. Changes during aging and their association with malnutrition. Journal of Clinical Gerontology and Geriatrics 2015;6:78-84.
Gong Z, Muzumdar RH. Pancreatic Function, Type 2 Diabetes, and Metabolism in Aging. Int J Endocrinol 2012;2012:320482.
De Tata V. Age-related impairment of pancreatic Beta-cell function: pathophysiological and cellular mechanisms. Fendo 2014; 5:138.
Park S, Kim CS, Min J, Lee SH, Jung YS. A high-fat diet increases oxidative renal injury and protein glycation in D-galactose-induced aging rats and its prevention by Korea red ginseng. J Nutr Sci Vitaminol (Tokyo) 2014; 60:159-66.
Anantharaju A, Feller A, Chedid A. Aging liver. Gerontology 2002;48:343-53.
Ahangarpour A, Oroojan AA, Khorsandi L, Najimi SA. Pancreatic protective and hypoglycemic effects of Vitex agnus-castus L. fruit hydroalcoholic extract in D-galactose-induced aging mouse model. Res Pharm Sci 2017;12:137.
Kim H, Kisseleva T, Brenner DA. Aging and liver disease. Curr Opin Gastroenterol 2015;31:184.
Ruiz-Ruiz JC, Moguel-Ordoñez YB, Matus-Basto AJ, Segura-Campos MR. Antidiabetic and antioxidant activity of Stevia rebaudiana extracts (Var. Morita) and their incorporation into a potential functional bread. J Food Sci Technol 2015;52:7894-903.
Xiao JH, Xiao DM, Chen DX, Xiao Y, Liang ZQ, Zhong JJ. Polysaccharides from the medicinal mushroom Cordyceps taii show antioxidant and immunoenhancing activities in a D-galactose-induced aging mouse model. Evid Based Complement Alternat Med 2012;2012.
Chen W, Feng L, Shen Y, Su H, Li Y, Zhuang J, et al. Myricitrin inhibits acrylamide-mediated cytotoxicity in human Caco-2 cells by preventing oxidative stress. Biomed Res Int 2013;2013.
Huang Q, Gao B, Wang L, Hu YQ, Lu WG, Yang L, et al. Protective effects of myricitrin against osteoporosis via reducing reactive oxygen species and bone-resorbing cytokines. Toxicol Appl Pharmacol 2014;280:550-60.
Dillin A, Gottschling DE, Nyström T. The good and the bad of being connected: the integrons of aging. Curr Opin Cell Biol 2014; 26:107-12.
Domitrović R, Rashed K, Cvijanović O, Vladimir-Knežević S, Škoda M, Višnić A. Myricitrin exhibits antioxidant, anti-inflammatory and antifibrotic activity in carbon tetrachloride-intoxicated mice. Chem Biol Interact 2015; 230:21-9.
Ahangarpour A, Oroojan AA, Rezae M, Khodayar MJ, Alboghobeish S, Zeinvand M. Effects of butyric acid and arsenic on isolated pancreatic islets and liver mitochondria of male mouse. Gastroenterol Hepatol Bed Bench 2017;10:44.
Onishi Y, Hayashi T, Sato KK, Ogihara T, Kuzuya N, Anai M, et al. Fasting tests of insulin secretion and sensitivity predict future prediabetes in Japanese with normal glucose tolerance. J Diabetes Investig 2010;1:191-5.
Ma Y, Wang Y, Huang Q, Ren Q, Chen S, Zhang A, et al. Impaired cell function in Chinese newly diagnosed type 2 diabetes mellitus with hyperlipidemia. J Diabetes Res 2014;2014.
Ahangarpour A, Oroojan AA, Khorsandi L, Lamoochi Z. Effect of Hydroalcoholic Extract of Purslane (Portulaca Oleracea L.) on Diabetic Variables in D-Galactose Induced Aging Mouse Model. Acta Endocrinol (Buchar) 2018;14:24.
Zhao X, Zhang J, Tong N, Chen Y, Luo Y. Protective effects of berberine on doxorubicin-induced hepatotoxicity in mice. Biol Pharm Bull 2012;35:796-800.
Herbach N, Bergmayr M, Göke B, Wolf E, Wanke R. Postnatal development of numbers and mean sizes of pancreatic islets and beta-cells in healthy mice and giprdn transgenic diabetic mice. PLoS One 2011;6.
Robertson RP. Chronic oxidative stress as a central mechanism for glucose toxicity in pancreatic islet beta cells in diabetes. J Biol Chem 2004;279:42351-4.
Ahangarpour A, Oroojan AA, Heidari H. Effects of exendin-4 on male reproductive parameters of d-galactose induced aging mouse model. World J Mens Health 2014;32:176-83.
Zhou YY, Ji XF, Fu JP, Zhu XJ, Li RH, Mu CK, et al. Gene transcriptional and metabolic profile changes in mimetic aging mice induced by D-galactose. PloS one 2015;10: e0132088.
Wang S, Sun QQ, Xiang B, Li XJ. Pancreatic islet cell autophagy during aging in rats. Clin Invest Med 2013: E72-80.
Szoke E, Shrayyef MZ, Messing S, Woerle HJ, Van Haeften TW, Meyer C, et al. Effect of aging on glucose homeostasis: accelerated deterioration of β-cell function in individuals with impaired glucose tolerance. Diabetes Care 2008;31:539-43.
Kajimoto Y, Kaneto H. Role of oxidative stress in pancreatic β-cell dysfunction. In Mitochondrial Pathogenesis 2004;168-176.
Ahangarpour A, Oroojan AA, Khorsandi L, Kouchak M, Badavi M. Solid Lipid Nanoparticles of Myricitrin Have Antioxidant and Antidiabetic Effects on Streptozotocin-Nicotinamide-Induced Diabetic Model and Myotube Cell of Male Mouse. Oxid Med Cell Longev 2018;2018.
Zhou Y, Xu Q, Dong Y, Zhu S, Song S, Sun N. Supplementation of mussel peptides reduces aging phenotype, lipid deposition and oxidative stress in D-galactose-induce aging mice. J Nutr Health Aging 2017;21:1314-20.
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