Evaluation of GKN1 and GKN2 gene expression as a biomarker of gastric cancer
Gastroenterology and Hepatology from Bed to Bench,
Vol. 11 No. Supplement 1 (2018),
11 December 2018
,
Page s140--s145
https://doi.org/10.22037/ghfbb.v0i0.1540
Abstract
Aim: The aim of this study was to investigate the expression of GKN1 and GKN2 genes as probable biomarkers for gastric cancer.
Background: Gastric cancer is a multifactorial process characterized by the uncontrolled growth and dissemination of abnormal cells. Survival rates of gastric cancer tend to be poor, a plausible explanation is a combination of a late-stage diagnosis and limited access to treatment. In this regard, finding relevant and measurable biomarkers is urgently needed.
Methods: 27 samples of gastric cancer tissues were enrolled into this study, according to their pathological responses. The alteration of genes expression were evaluated by Real-Time PCR technique.
Results: Our findings showed the significant reduction of Gastrokin-1 and Gastrokine-2 genes expression in the cancerous specimens in comparison with the normal tissues. (P = 0.008 and P = 0.004 respectively).
Conclusion: Our findings showed the significant reduction of Gastrokin-1 and Gastrokine-2 genes expression in the cancerous specimens in comparison with the normal tissues. (P = 0.008 and P = 0.004 respectively).
Keywords: Gastric cancer, Gastrokine-1(GKN1), Gastrokine-2 (GKN2), Real-time PCR.
(Please cite as: Dokhaee F, Mazhari S, Galehdari M, Bahadori Monfared A, Baghaei K. Evaluation of GKN1 and GKN2 gene expression as a biomarker of gastric cancer. Gastroenterol Hepatol Bed Bench 2018;11(Suppl. 1):S140-S145).
- Gastric cancer
- Gastrokine-1(GKN1)
- Gastrokine-2 (GKN2)
- Real-time PCR.
How to Cite
References
Norouzinia M, Asadzadeh H, Shalmani HM, Al Dulaimi D, Zali MR. Clinical and histological indicators of proximal and distal gastric cancer in eight provinces of Iran. Asian Pac J Cancer Prev. 2012;13:5677-9.
Fock KM, Ang TL. Epidemiology of Helicobacter pylori infection and gastric cancer in Asia. J Gastroenterol Hepatol 2010;25:479-86.
Baghestani AR, Daneshva T, Pourhoseingholi MA, Asadzadeh H. Survival of Colorectal Cancer in the Presence of Competing- Risks - Modeling by Weibull Distribution. Asian Pac J Cancer Prev 2016;17:1193-96.
Nazemalhosseini Mojarad E, Farahani RK, Haghighi MM, Aghdaei HA, Kuppen PJ, Zali MR. Clinical implications of BRAF mutation test in colorectal cancer. Gastroenterol Hepatol Bed Bench. 2013;6:6-13.
Mousavi SM, Gouya MM, Ramazani R, Davanlou M, Hajsadeghi N, Seddighi Z. Cancer incidence and mortality in Iran. Ann Oncol 2009;20:556-63.
Kashfi SM, Behboudi Farahbakhsh F, Nazemalhosseini Mojarad E, Mashayekhi K, Azimzadeh P, Romani S, et al. Interleukin-16 polymorphisms as new promising biomarkers for risk of gastric cancer. Tumour Biol. 2016;37:2119-26.
Karbalaei R, Piran M, Rezaei-Tavirani M, Asadzadeh-Aghdaei H, Heidari MH. A systems biology analysis protein-protein interaction of NASH and IBD based on comprehensive gene information. Gastroenterol Hepatol Bed Bench. 2017;10:194-201.
Dai J, Zhang N, Wang J, Chen M, Chen J. Gastrokine-2 is downregulated in gastric cancer and its restoration suppresses gastric tumorigenesis and cancer metastasis. Tumour Biol 2014;35:4199-207.
Toback FG, Walsh-Reitz MM, Musch MW, Chang EB, Del Valle J, Ren H, et al. Peptide fragments of AMP-18, a novel secreted gastric antrum mucosal protein, are mitogenic and motogenic. Am J Physiol Gastrointest Liver Physiol 2003;285:G344-53.
Yoon JH, Seo HS, Choi SS, Chae HS, Choi WS, Kim O, et al. Gastrokine 1 inhibits the carcinogenic potentials of
Helicobacter pylori CagA. Carcinogenesis 2014;35:2619-29.
Yoon JH, Choi YJ, Choi WS, Nam SW, Lee JY, Park WS. Functional analysis of the NH2-terminal hydrophobic region and BRICHOS domain of GKN1. Biochem Biophys Res Commun 2013;440:689-95.
Fahlbusch FB, Ruebner M, Huebner H, Volkert G, Bartunik H, Winterfeld I, et al. Trophoblast expression dynamics of the tumor suppressor gene gastrokine 2. Histochem Cell Biol 2015;144:281-91.
Moss SF, Lee JW, Sabo E, Rubin AK, Rommel J, Westley BR, et al. Decreased expression of gastrokine 1 and the trefoil factor interacting protein TFIZ1/GKN2 in gastric cancer: influence of tumor histology and relationship to prognosis. Clin Cancer Res 2008;14:4161-7.
Yoon JH, Kang YH, Choi YJ, Park IS, Nam SW, Lee JY, et al. Gastrokine 1 functions as a tumor suppressor by inhibition of epithelial-mesenchymal transition in gastric cancers. J Cancer Res Clin Oncol 2011;137:1697-704.
Norouzinia M, Asadzadeh H, Shalmani HM, Al Dulaimi D, Zali MR. Clinical and histological indicators of proximal and distal gastric cancer in eight provinces of Iran. Asian Pac J Cancer Prev 2012;13:5677-79.
Ichikawa T٫ Ishihara K. Protective effects of gastric mucus. In: Tonino P٫ ed. Gastritis and gastric cancer - new insights in gastroprotection, diagnosis and treatments. InechOpen. 2011. P.1-25.
Kim O, Yoon JH, Choi WS, Ashktorab H, Smoot DT, Nam SW, et al. GKN2 contributes to the homeostasis of gastric mucosa by inhibiting GKN1 activity. J Cell Physiol 2014;229:762-71.
Di Stadio CS, Altieri F, Minopoli G, Miselli G, Rippa E, Arcari P. Role of human GKN1 on APP processing in gastric cancer. Biochimie 2017;135:149-53.
Yoon JH, Kang YH, Choi YJ, Park IS, Nam SW, Lee JY, et al. Gastrokine 1 functions as a tumor suppressor by inhibition of epithelial-mesenchymal transition in gastric cancers. J Cancer Res Clin Oncol 2011;137:1697-704.
Mao W, Chen J, Peng TL, Yin XF, Chen LZ, Chen MH. Helicobacter pylori infection and administration of non-steroidal anti-inflammatory drugs down-regulate the expression of gastrokine-1 in gastric mucosa. Turk J Gastroenterol 2012;23:212-9.
Baus-Loncar M, Lubka M, Pusch CM, Otto WR, Poulsom R, Blin N. Cytokine regulation of the trefoil factor family binding protein GKN2 (GDDR/TFIZ1/blottin)
in human gastrointestinal epithelial cells. Cell Physiol Biochem 2007;20:193-204.
Amer A Hasan, Mehri Igci, Ersin Borazan, Rozhgar A, Khailany, Emine Bayraktar, et al. Down-Regulated Gene Expression of GKN1 and GKN2 as Diagnostic Markers for Gastric Cancer. International Scholarly and Scientific Research & Innovation 2015;9:532-5.
Oien KA, McGregor F, Butler S, Ferrier RK, Downie I, Bryce S, et al. Gastrokine 1 is abundantly and specifically expressed in superficialgastric epithelium, down-regulated in gastric carcinoma, and shows high evolutionary conservation. J Pathol 2004;203:789-97.
Rippa E, La Monica G, Allocca R, Romano MF, De Palma M, Arcari P. Overexpression of gastrokine 1 in gastric cancer cells induces Fas-mediated apoptosis. J Cell Physiol 2011;226:2571-8.
Menheniott TR, Kurklu B, Giraud AS. Gastrokines: stomach-specific proteins with putative homeostatic and tumor suppressor roles. Am J Physiol Gastrointest Liver Physiol 2013;304:G109-21.
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