Designing a novel ELISA method to increase sensitivity and specificity of Helicobacter pylori whole cell antigen detection
Gastroenterology and Hepatology from Bed to Bench,
Vol. 11 No. 4 (2018),
2 September 2018
,
Page 333-342
https://doi.org/10.22037/ghfbb.v11i4.1451
Abstract
Aim: In this research, we designed a direct Enzyme Linked Immunoassay method to detect Helicobacter pylori antigens in stool
specimens.
Background: Helicobacter pylori infection as the worldwide problem is related to many gastrointestinal disorders such as gastritis,
gastric cancer, non-ulcer disease, peptic ulcer disease and duodenal ulcer.
Methods: We produced and purified recombinant CagA and NapA antigens in Escherichia coli and extracted their antibodies from a
panel of positive sera specimens. We designed a novel enzyme linked immunoassay direct method in combination with the whole cell
for the qualitative and quantitative detection of Helicobacter pylori antigens in human stool. Assay performance was evaluated by
histopathology staining and urease activity.
Results: The sensitivity and specificity of assay was determined as 91.7 [95% confidence interval: 89.3–95.6%] and 93.1% [95% CI:
91.2–96.4%], respectively. Novel ELISA exhibits enhanced sensitivity and specificity of Helicobacter pylori detection in comparison
with another commercially available kit.
Conclusion: Combination of the recombinant antigens and whole cell of Helicobacter pylori in immunoassay designing is a new
approach about early diagnosis, treatment and fallowing up of the Helicobacter pylori infected patients, especially in peptic cancer
cases.
Keywords: CagA protein, Helicobacter pylori, Neutrophil activating protein A, Enzyme-linked immunosorbent assay.
(Please cite as: Kamarehei F, Khabiri AR, Saeedi Jam M, Soleimani M, Alikhani MY. Designing a novel ELISA
method based on CagA, NapA recombinant antigens to increase sensitivity and specificity of Helicobacter pylori
whole cell antigen detection. Gastroenterol Hepatol Bed Bench 2018;11(4):333-342).
- Helicobacter pylori
- Cytotoxin-associated gene A
- Neutrophil activating protein A
- Enzyme-linked immunosorbent assay
How to Cite
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