Determination of virulence determinants of Escherichia coli strains isolated from patients with colorectal cancer compared to the healthy subjects
Gastroenterology and Hepatology from Bed to Bench,
Vol. 12 No. 1 (2019),
29 January 2019
Background: Colon cancer is one of the most important malignancies in recent years. Escherichia coli (E.coli) is the most important infectious agents associated with colon cancer that has numerous virulence factors such as iron uptake and adhesion factors involved in the process of inflammation and colorectal cancer. Several virulence factors such as iron and adhesion factors can contribute to the disease caused by E. coli.
Material and Method: Of the three normal, inflammatory bowel disease (IBD), colorectal cancer (CRC) groups, 40 E. coli strains isolated after confirmation by biochemical and molecular methods. After determining the phylogroup, the frequency of virulence factors was measured by a PCR method. Biofilm test was also performed on phenotypes on all three groups.
Result: In the determination of the phylogroup associated with the bacteria, the CRC had a maximum incidence of phylogroups B2 and A. In the analysis of fimH, papA, papC, iutA, ireA and fyuA the highest frequency of these genes was observed in these two phylogroups. Statistical analyzes were only significant for the ireA gene. There was also no significant difference in Biofilm productions in E. coli strains among the three groups.
Conclusion: Contrary to previous studies that focused solely on E. coli toxins, we found that factors such as iron uptake and adhesion can also be effective in developing IBD and colorectal cancer. Also found that the formation of biofilm is a special feature of E. coli isolated from the colon.
- Escherichia coli
- colorectal cancer
- Virulence factor
How to Cite
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