Research/Original Article- Biochemistry


The Association of Transcript Levels of Proinflammatory Cytokines in Adipose Tissues with Various Adiposity Indices in Women with Obesity

Masoume Aliabadi, Sadra Samavarchi Tehrani , Mehrnoosh Shanaki

Archives of Medical Laboratory Sciences, Vol. 8 (2022), , Page 1-10 (e1)
https://doi.org/10.22037/amls.v8.35941

Background and Aim: To investigate the association of adipose tissue transcript levels of IL-1β, IL-6, TNF-α, and MCP-1 with various adiposity indices in obese women.


Methods: Real-time PCR was carry out to investigate the mRNA expression level of the mentioned genes in VAT and SAT from all participants.


Results: The results presented higher mRNA levels of IL-6 and MCP-1 in SAT and VAT of obese women, compared to normal-weight women. As well, results showed a positive correlation of IL-6 and MCP-1 with HOMA-IR. Obesity indices including BMI, hip, and WHtR were considerably higher in the obese group in comparison with the control group. More importantly, we observed a positive correlation of mRNA expression of these pro-inflammatory factors in adipose tissues with some obesity indices.


Conclusion: We have shown here that adipose tissue transcript levels of pro-inflammatory cytokines were significantly higher in obese participate than non-obese participants. In obese individuals, this proinflammatory molecules was significantly correlated with various obesity indices. These results suggest that targeting obesity and adipose tissue could prevent the high expression of cytokine.


*Corresponding Author: Dr. Mehrnoosh Shanaki; Email: shanaki_m@sbmu.ac.ir


Please cite this article as: Aliabadi M, Samavarchi Tehrani S, Shanaki M. The Association of Transcript Levels of Proinflammatory Cytokines in Adipose Tissues with Various Adiposity Indices in Women with Obesity. Arch Med Lab Sci. 2022;8:1-10 (e1). https://doi.org/10.22037/amls.v8.35941

Research/Original Article- Virology


Seroprevalence of SARS-CoV-2 among ICU Healthcare Workers

Mitra Jafari, Taravat Bamdad, Samira Asli, Fateme Moghbeli, Saeid Amel Jamedar

Archives of Medical Laboratory Sciences, Vol. 8 (2022), , Page 1-7 (e2)
https://doi.org/10.22037/amls.v8.36039

Background and Aim: Coronavirus disease-2019 (COVID-19) has affected most countries in the world. The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) was first reported in late December 2019 in Wuhan, China, and then spread around the world. In Iran, the first case with a definitive test was reported on February 20 in Qom province and a month later in March, the first case was reported in Mashhad. Hospital staff is among the most susceptible groups who are more likely to be infected due to direct contact with patients. The aim of this study was to evaluate the serum prevalence of antibodies against COVID-19 in patients with or without clinical symptoms working in the Intensive Care Unit (ICU) of hospitals affiliated with Mashhad University of Medical Sciences.


Methods: The present study is a cross-sectional study of sero-epidemiological type that was conducted to investigate the serological prevalence of COVID-19 in the period from August 25 to September 30, 2020, in Mashhad. A total of 300 serum samples were collected from the ICU staff of the hospitals affiliated with Mashhad University of Medical Sciences. Sero-prevalence and 95% confidence interval (CI) were calculated for all individuals who were stratified by job title, COVID-19 risk of exposure, direct contact with patients, hospitals, and intensity of care. The significance level was set generally at p < 0.05. Data were analyzed using SPSS V.25.


Results: The total sero-prevalence of IgG N antibody was 30.67% in COVID-19 ICU healthcare workers. The most prevalence (35.21 %) was recorded in nurse assistances who have the most contact with patients. Among asymptomatic individuals, 17.1% showed to be positive without any symptoms which emphasize the risk of the virus spread from this population who are not aware of their infection. Odds ratios and 95% confidence intervals were reported in the results. The significant difference indicates a statistically significant association with a p-value less than 0.05 (P-value=0.001).


Conclusion: Our study showed that less contact with patients with COVID-19 results in a lower risk of infection. Also, there is a high percentage of positive people among the staff who did not show any symptoms of the disease.


*Corresponding Authors:


Please cite this article as: Jafari M, Bamdad T, Asli S, Moghbeli F, Amel Jamedar S. Seroprevalence of SARS-CoV-2 among ICU Healthcare Workers. Arch Med Lab Sci. 2022;8:1-7 (e2). https://doi.org/10.22037/amls.v8.36039