• Logo
  • SBMUJournals

Expression Levels of lncRNAs in the Patients with the Renal Transplant Rejection

Mohsen Nafar, Shiva Kalantari, Sayyed Mohammad Hossein Ghaderian, Mir Davood Omrani, Hamid Fallah, Shahram Arsang-Jang, Tahereh Abbasi, Shiva Samavat, Noshin Dalili, Mohammad Taheri, Soudeh Ghafouri-Fard



Purpose: Long non-coding RNAs (lncRNAs) include a vast portion of human transcripts. They exert regulatory roles in immune responses and participate in diverse biological functions. Recent studies indicated dysregulation of lncRNAs in the process of transplant rejection. In the current study, we aimed at identification of the expression of five lncRNAs (OIP5-AS1, FAS-AS1, TUG1, NEAT1 and PANDAR) in association with the process of transplant rejection.

Material and Methods: We assessed expression of these lncRNAs in the peripheral blood of 61 kidney transplant receivers including 29 transplant rejected patients and 32 transplant non-rejected patients using real time PCR technique.

Results: Expression of FAS-AS1 was significantly higher in rejected group compared to non-rejected group in males, however, differences between case and control groups were insignificant among females. For other lncRNAs no significant differences were detected between two study groups. Quantile regression model showed that patients’ gender was an important parameter in determination of FAS-AS1 expression (Beta=-9.46, t=-2.82, P=0.007) but not for other lncRNAs expressions. Significant pairwise correlations were detected between expression levels of lncRNAs in a disease related manner.

Conclusion: Based on the higher expression of FAS-AS1 in patients with transplant rejection, this lncRNA might be associated with the pathogenesis of renal transplant rejection.


kidney transplant, rejection, lncRNA, OIP5-AS1, FAS-AS1, TUG1, NEAT1, PANDAR


Saidi RF, Broumand B. Current Challenges of Kidney Transplantation in Iran: Moving Beyond The "Iranian Model". Transplantation. 2018;102(8):1195-1197.

Jalalzadeh M, Mousavinasab N, Peyrovi S, Ghadiani MH. The impact of acute rejection in kidney transplantation on long-term allograft and patient outcome. Nephro-urology monthly. 2015;7(1):e24439.

Ge YZ, Xu T, Cao WJ, et al. A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 2017;44(3):1213-1223.

Fernandes JCR, Acuna SM, Aoki JI, Floeter-Winter LM, Muxel SM. Long Non-Coding RNAs in the Regulation of Gene Expression: Physiology and Disease. Non-coding RNA. 2019;5(1).

Carpenter S, Aiello D, Atianand MK, et al. A long noncoding RNA mediates both activation and repression of immune response genes. Science (New York, NY). 2013;341(6147):789-792.

Sui W, Lin H, Peng W, et al. Molecular dysfunctions in acute rejection after renal transplantation revealed by integrated analysis of transcription factor, microRNA and long noncoding RNA. Genomics. 2013;102(4):310-322.

Chen W, Peng W, Huang J, et al. Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation. Molecular medicine reports. 2014;10(4):2210-2216.

Zou XF, Song B, Duan JH, Hu ZD, Cui ZL, Yang T. PRINS Long Noncoding RNA Involved in IP-10-Mediated Allograft Rejection in Rat Kidney Transplant. Transplantation proceedings. 2018;50(5):1558-1565.

Yang N, Chen JQ, Zhang H, et al. LncRNA OIP5-AS1 loss-induced microRNA-410 accumulation regulates cell proliferation and apoptosis by targeting KLF10 via activating PTEN/PI3K/AKT pathway in multiple myeloma. Cell Death Dis. 2017;8.

Hawkins PT, Stephens LR. PI3K signalling in inflammation. Bba-Mol Cell Biol L. 2015;1851(6):882-897.

Nikolov NP, Shimizu M, Cleland S, et al. Systemic autoimmunity and defective Fas ligand secretion in the absence of the Wiskott-Aldrich syndrome protein. Blood. 2010;116(5):740-747.

Ramaswamy M, Siegel RM. A FAScinating receptor in self-tolerance. Immunity. 2007;26(5):545-547.

Zhang HF, Li H, Ge A, Guo EY, Liu SX, Zhang LJ. Long non-coding RNA TUG1 inhibits apoptosis and inflammatory response in LPS-treated H9c2 cells by down-regulation of miR-29b. Biomed Pharmacother. 2018;101:663-669.

Imamura K, Imamachi N, Akizuki G, et al. Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli. Molecular cell. 2014;53(3):393-406.

Puvvula PK, Desetty RD, Pineau P, et al. Long noncoding RNA PANDA and scaffold-attachment-factor SAFA control senescence entry and exit. Nature communications. 2014;5:5323.

Dastmalchi R, Ghafouri-Fard S, Omrani MD, Mazdeh M, Sayad A, Taheri M. Dysregulation of long non-coding RNA profile in peripheral blood of multiple sclerosis patients. Multiple sclerosis and related disorders. 2018;25:219-226.

Racusen L, Rayner D, Trpkov K, Olsen S, Solez K. The Banff classification of renal allograft pathology: where do we go from here? Transplantation proceedings. 1996;28(1):486-488.

Sayad A, Taheri M, Omrani MD, Fallah H, Kholghi Oskooei V, Ghafouri-Fard S. Peripheral expression of long non-coding RNAs in bipolar patients. Journal of affective disorders. 2019;249:169-174.

Qiu J, Chen Y, Huang G, Zhang Z, Chen L, Na N. Transforming growth factor-beta activated long non-coding RNA ATB plays an important role in acute rejection of renal allografts and may impacts the postoperative pharmaceutical immunosuppression therapy. Nephrology (Carlton, Vic). 2017;22(10):796-803.

Yan MD, Hong CC, Lai GM, Cheng AL, Lin YW, Chuang SE. Identification and characterization of a novel gene Saf transcribed from the opposite strand of Fas. Human molecular genetics. 2005;14(11):1465-1474.

Sehgal L, Mathur R, Braun FK, et al. FAS-antisense 1 lncRNA and production of soluble versus membrane Fas in B-cell lymphoma. Leukemia. 2014;28(12):2376-2387.

Wang T, Dong CM, Stevenson SC, et al. Overexpression of soluble Fas attenuates transplant arteriosclerosis in rat aortic allografts. Circulation. 2002;106(12):1536-1542.

Safari MR, Komaki A, Arsang-Jang S, Taheri M, Ghafouri-Fard S. Expression Pattern of Long Non-coding RNAs in Schizophrenic Patients. Cellular and molecular neurobiology. 2019;39(2):211-221.

Song J, Rutherford T, Naftolin F, Brown S, Mori G. Hormonal regulation of apoptosis and the Fas and Fas ligand system in human endometrial cells. Mol Hum Reprod. 2002;8(5):447-455.

DOI: http://dx.doi.org/10.22037/uj.v0i0.5456


  • There are currently no refbacks.