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Human Papilloma Virus DNA in Tumor Tissue and Urine in Different Stage of Bladder Cancer

Babak Javanmard, Mohammad Reza Barghi, Davar Amani, Morteza Fallah Karkan, Mohammad Mohsen Mazloomfard



Purpose: There are some previous reports on the relationship between pathological grades and HPV detection. To determine the Human Papilloma Virus(HPV)  DNA in Tumor Tissue and Urine in Different Stage of Bladder Cancer conducted this study.

Materials and Methods: Polymerase chain reaction (PCR) was used to detect general HPV and HPV16 and 18 subtypes in 110 bladder tumor tissue and urine specimens of patients with TCC of bladder between January 2014 to May 2016 that underwent transurethral resection of bladder tumor. Exclusion criteria were genital wart and cases with immunosupression.

Results: Mean age of 110 patients was 61.6±10 years and fourteen (12.7%) of patients were female. PCR for general HPV primer in bladder tumor tissue was positive in 3 (9.4%), 22 (38.6%) and 15 (71.4%) of Ta, T1 and T2 bladder tumors, respectively (p<0.001). PCR for HPV16 in bladder tumor tissue was positive in 2(6.3%), 10 (17.5%) and 13 (61.9%) and PCR for HPV18 in bladder tumor tissue was positive in 1 (3.1%), 14 (24.6%) and 12 (57.1%) of Ta, T1 and T2 bladder tumors, respectively (p<0.001, p<0.001). Thirty seven (33.6%) of urine specimens were positive for general HPV using PCR and HPV16 and 18 subtypes were positive in 17 (15.5%) and 14 (12.7%) of urine specimens, respectively.

Conclusion: HPV infection may be associated with higher stages and grades of bladder carcinomas. Urine sampling for HPV detection is is as reliable as tumor tissue sample which could be considered for prognostic and follow up implications.


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DOI: http://dx.doi.org/10.22037/uj.v0i0.4181


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