Impact of Four Week Swimming Exercise with Alpha-Tocopherol Supplementation on Fertility Potential in Healthy Rats

Abolfazl Kalantari, Abbas Saremi, Nader Shavandi, Ali Foroutan Nia



Purpose: The aim of this study was to evaluate the effect of 4 week intensive swimming exercise and alpha-tocopherol supplementation on testicular oxidative stress and spermatogenesis in rats.
Materials and Methods: 40 male rats were randomly assigned to Control (C), Sham (S), Exercise (E) and Exercise + supplement (ES) groups. Exercise training performed for 4 weeks (1session/day, 6days/week). Each session included 180 minutes of swimming. In ES group, alpha-ocopherol was injected at a dose of 50 mg/kg/day. 48 hours after last training session, all rats were killed and gonads of them were removed from their body for histological and biochemical assays. All statistical analysis was performed by SPSS 16. P values less than 0.05 were considered as statistically significant.
Results: Total testicular antioxidant capacity increased significantly in E (P = .003) and ES groups (P = .001) whereas there was no significant difference between C and E group in testicle Malondialdehyde (a lipid peroxidation marker) level (P = .999) and spermatogenesis quality (P = .381). Testicle Malondialdehyde level decreased (P = .009) and spermatogenesis quality was improved significantly in ES group (P = .001).
Conclusion: Alpha-tocopherol supplementation is effective in order to improve spermatogenesis process in athletes who exercise with high intensity.

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Auger J, Eustache F, Andersen A, et al. Sperm morphological defects related to environment, lifestyle and medical history of 1001 male partners of pregnant women from four European cities. Human reproduction. 2001;16:2710-7.

Bansal AK, Bilaspuri G. Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International. 2010;2011.

Jungwirth A, Giwercman A, Tournaye H, et al. European Association of Urology guidelines on Male Infertility: the 2012 update. European urology. 2012;62:324-32.

Tartibian B, Maleki BH. The effects of honey supplementation on seminal plasma cytokines, oxidative stress biomarkers, and antioxidants during 8 weeks of intensive cycling training. Journal of andrology. 2012;33:449-61.

Zini A, Al-Hathal N. Antioxidant therapy in male infertility: fact or fiction? Asian journal of andrology. 2011;13:374.

Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. The world journal of men's health. 2014;32:1-17.

Manna I, Jana K, Samanta PK. Effect of different intensities of swimming exercise on testicular oxidative stress and reproductive dysfunction in mature male albino Wistar rats. Indian journal of experimental biology. 2004;42:816-22.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry. 1979;95:351-8.

Dillioglugil M, Maral Kir H, Gulkac M, et al. Protective effects of increasing vitamin E and a doses on cisplatin-induced oxidative damage to kidney tissue in rats. Urologia internationalis. 2005;75:340-4.

Benzie IF, Strain J. [2] Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in enzymology. 1999;299:15-27.

Li M, Zhou L, Yang D, Li T, Li W. Biochemical composition and antioxidant capacity of extracts from Podophyllum hexandrum rhizome. BMC complementary and alternative medicine. 2012;12:263.

Dieckmann KP, Linke J, Pichlmeier U, Kulejewski M, Loy V. Spermatogenesis in the contralateral testis of patients with testicular germ cell cancer: histological evaluation of testicular biopsies and a comparison with healthy males. BJU international. 2007;99:1079-85.

Janett F, Burkhardt C, Burger D, Imboden I, Hässig M, Thun R. Influence of repeated treadmill exercise on quality and freezability of stallion semen. Theriogenology. 2006;65:1737-49.

Safarinejad MR, Azma K, Kolahi AA. The effects of intensive, long-term treadmill running on reproductive hormones, hypothalamus–pituitary–testis axis, and semen quality: a randomized controlled study. Journal of Endocrinology. 2009;200:259-71.

Maleki BH, Tartibian B, Vaamonde D. The effects of 16 weeks of intensive cycling training on seminal oxidants and antioxidants in male road cyclists. Clinical Journal of Sport Medicine. 2014;24:302-7.

Jackson MJ, Pye D, Palomero J. The production of reactive oxygen and nitrogen species by skeletal muscle. Journal of Applied Physiology. 2007;102:1664-70.

Radak Z, Zhao Z, Koltai E, Ohno H, Atalay M. Oxygen consumption and usage during physical exercise: the balance between oxidative stress and ROS-dependent adaptive signaling. Antioxidants & redox signaling. 2013;18:1208-46.

Vinña J, Gomez‐Cabrera MC, Lloret A, et al. Free radicals in exhaustive physical exercise: mechanism of production, and protection by antioxidants. IUBMB life. 2000;50:271-7.

Lambertucci RH, Levada-Pires AC, Rossoni LV, Curi R, Pithon-Curi TC. Effects of aerobic exercise training on antioxidant enzyme activities and mRNA levels in soleus muscle from young and aged rats. Mechanisms of ageing and development. 2007;128:267-75.

Ourique GM, Saccol EM, Pês TS, et al. Protective effect of vitamin E on sperm motility and oxidative stress in valproic acid treated rats. Food and Chemical Toxicology. 2016;95:159-67.

Chigurupati S, Son TG, Hyun D-H, et al. Lifelong running reduces oxidative stress and degenerative changes in the testes of mice. Journal of Endocrinology. 2008;199:333-41.

Kinnunen S, Atalay M, Hyyppä S, Lehmuskero A, Hänninen O, Oksala N. Effects of prolonged exercise on oxidative stress and antioxidant defense in endurance horse. J Sports Sci Med. 2005;4:415-21.

Vaamonde D, Da Silva-Grigoletto ME, García-Manso JM, Vaamonde-Lemos R, Swanson RJ, Oehninger SC. Response of semen parameters to three training modalities. Fertility and sterility. 2009;92:1941-6.

Schneider C. Chemistry and biology of vitamin E. Molecular nutrition & food research. 2005;49:7-30.

Momeni HR, Eskandari N. Effect of vitamin E on sperm parameters and DNA integrity in sodium arsenite-treated rats. International Journal of Reproductive BioMedicine. 2012;10.

Moslemi MK, Tavanbakhsh S. Selenium-vitamin E supplementation in infertile men: effects on semen parameters and pregnancy rate. Int J Gen Med. 2011;4:99-104.

Rengaraj D, Hong YH. Effects of dietary vitamin E on fertility functions in poultry species. Int J Mol Sci. 2015;16:9910-21.

Kothari S, Thompson A, Agarwal A, du Plessis SS. Free radicals: their beneficial and detrimental effects on sperm function. Indian journal of experimental biology. 2010;48:425-35.


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