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Contamination of tap water sources to Acanthamoeba spp. in selected cities of Hormozgan province, Iran

Maryam Niyyati, Zaynab Jonaydi, Hamed Behniafar, Naser Valai
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Abstract

Background   

Free-living amoeba (FLA) such as Acanthamoeba are ubiquitous and amphizoic protozoan parasites. These parasites can cause severe human infections. Water resources are one of the important habitats for FLAs. Waters in tanks and wells can be a habitat for Acanthamoeba species, particularly in warm climates. The present study was conducted to evaluate the presence of Acanthamoeba species in tap water resources of selected cities of Hormozgan Province, Southern Iran.

Material and methods

In this cross sectional study, 50 water samples (approximately 500 mL) were collected from tap water resources (25 samples from tanks and 25 samples from wells) of Hormozgan province. The samples were filtered, cultured on 1.5% non-nutrient agar medium. After incubation at room temperature, cloning was performed. Investigation of Acanthamoeba species was performed using the microscopic method and page key.

Results

Out of 50 samples, 14 samples (28%) were found positive. Out of 25 examined well samples 9 samples (36%) were found contaminated with Acanthamoeba spp., while only 5 (20%) of 25 tank samples were found positive. All samples were cloned successfully.

Conclusion

The high occurrence of Acanthamoeba spp. in water resources of Hormozgan province could be as a hazard for public health of the region, and using effective disinfectants and accurate monitoring may be a good suggestion.

 

 



Keywords

Acanthamoeba; Water resources;Hormozgan province

References

Visvesvara GS, Moura H, Schuster FL. Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol Med Microbiol 2007;50(1):1-26.

Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev 2003;16(2):273-307.

Nuprasert W, Putaporntip C, Pariyakanok L, Jongwutiwes S. Identification of a novel T17 genotype of Acanthamoeba from environmental isolates and T10 genotype causing keratitis in Thailand. J Clin Microbiol 2010;48(12):4636-40.

Marciano-Cabral F, Jamerson M, Kaneshiro ES. Free-living amoebae, Legionella and Mycobacterium in tap water supplied by a municipal drinking water utility in the USA. J Water Health 2010;8(1):71-82.

De Jonckheere JF. Molecular identification of free-living amoebae of the Vahlkampfiidae and Acanthamoebidae isolated in Arizona (USA). Eur J Protistol 2007;43(1):9-15.

Edagawa A, Kimura A, Kawabuchi-Kurata T, Kusuhara Y, Karanis P. Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan. Parasitol Res 2009;105(4):1109-17.

Alizadeh H, Apte S, El-Agha M-SH, Li L, Hurt M, Howard K, et al. Tear IgA and serum IgG antibodies against Acanthamoeba in patients with Acanthamoeba keratitis. Cornea 2001;20(6):622-7.

Chappell CL, Wright JA, Coletta M, Newsome AL. Standardized Method of MeasuringAcanthamoeba Antibodies in Sera from Healthy Human Subjects. Clin Diagn Lab Immunol 2001;8(4):724-30.

Brindley N, Matin A, Khan NA. Acanthamoeba castellanii: high antibody prevalence in racially and ethnically diverse populations. Exp Parasitol 2009;121(3):254-6.

Stockman LJ, Wright CJ, Visvesvara GS, Fields BS, Beach MJ. Prevalence of Acanthamoeba spp. and other free-living amoebae in household water, Ohio, USA—1990–1992. Parasitol Res 2011;108(3):621-7.

Pelletier JS, Miller D, Liang B, Capriotti JA. In vitro efficacy of a povidone–iodine 0.4% and dexamethasone 0.1% suspension against ocular pathogens. J Cataract Refract Surg 2011;37(4):763-6.

Khan NA. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol Rev 2006;30(4):564-95.

Khan NA. Acanthamoeba: biology and pathogenesis. 1 st ed. London: Caister Academic Press; 2009.

Rezaeian M, Niyyati M. Pathogenic Free Living Amebas In Human. Tehran: TUMS Publication; 2010.

Winiecka-Krusnell J, Linder E. Bacterial infections of free-living amoebae. Res Microbiol 2001;152(7):613-9.

Horn M, Wagner M. Bacterial Endosymbionts of Free‐living Amoebae. J Eukaryot Microbiol 2004;51(5):509-14.

Rezeaian M, Farnia S, Niyyati M, Rahimi F. Amoebic keratitis in Iran (1997-2007). Iran J Parasitol 2007;2(3):1-6.

Wikipedia. https://www.wikipedia.org/ 2017.

Page FC. A new key to freshwater and soil gymnamoebae: with instructions for culture. 1988.

Badirzadeh A, Niyyati M, Babaei Z, Amini H, Badirzadeh H, Rezaeian M. Isolation of free-living amoebae from sarein hot springs in ardebil province, iran. Iran J Parasitol 2011;6(2):1.

Solgi R, Niyyati M, Haghighi A, Taghipour N, Tabaei SJS, Eftekhar M, et al. Thermotolerant Acanthamoeba spp. isolated from therapeutic hot springs in Northwestern Iran. J Water Health 2012;10(4):650-6.

Niyyati M, Lasgerdi Z, Lorenzo-Morales J. Detection and molecular characterization of potentially pathogenic free-living amoebae from water sources in Kish Island, Southern Iran. Microbiol Insights 2015:8(s1):1-6.

Behniafar H, Niyyati M, Lasjerdi Z, Dodangeh S. High occurrence of potentially pathogenic free living amoebae in water bodies of kaleybar and khodaafarin, east azerbaijan province. Curr World Environ 2015;10(Special Issue 1 (2015)):727-31.

Nazar M, Haghighi A, Niyyati M, Eftekhar M, Tahvildar-Biderouni F, Taghipour N, et al. Genotyping of Acanthamoeba isolated from water in recreational areas of Tehran, Iran. J water health 2011;9(3):603-8.

GHADAR GJS, Solhjoo K, NOROUZ NM, Rohi R, ZIA JS. Isolation and identification of free living amoeba (Naegleria and Acanthamoeba) in Shiraz water resources by morphological criteria. PARS J Med Sci 2012;10(3):33-41.


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