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A Simple, Inexpensive and Safe Method for DNA Extraction of Frigid and Clotted Blood Samples

Nasrin Mohammadi, Bahram Kazemi, Gholamreza Roozkhosh, Kamel Masoomi, Mohammad Taghi Farghadani
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Abstract

Background: Extraction of blood genomicDNAis one of the main approaches for clinical and molecular biology studies. Although several methods have been developed for extraction of blood genomic DNA, most of these methods consume long time and use expensive chemicals such as proteinase K and toxic organic solvent such as phenol and chloroform. The objective of this study was to developed easy and safe method forDNAextraction from clotted and frozen whole blood. This method has many advantages: time reducing, using inexpensive materials, without phenol and chloroform, achieving of high molecular weight and good quality genomicDNA.

Materials and Methods: DNA extraction was performed by two methods (new and phenol-chloroform method). Then quantity and quality parameters were evaluated by 1% agarose gel electrophoresis, Nano drop analysis and efficiency of Polymerase Chain Reaction (PCR).

Results: Extracted DNA from 500μL of blood samples were 457.7ng/μl and 212ng/μL and their purity (OD260/OD280) were 1.8 and 1.81 for new recommended and phenol–chloroform methods respectively. The PCR results indicated that D16S539 and CSF1PO loci were amplified.

Conclusion: These results shown that this method is simple, fast, safe and most economical.


Keywords

Genomic DNA extraction, Quality and quantity of DNA, Blood sample

References

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DOI: https://doi.org/10.22037/nbm.v3i3.9507