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Morphometric Plasticity of Nitric Oxide Containing Neurons in the Barrel Cortex of De-whiskered Rats

Mohammad Reza Afarinesh, Gila Behzadi
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Abstract

Background: The rodent somatosensory barrel cortex is an ideal model to examine the effect of experience-dependent plasticity on developing brain circuitry. Sensory deprivation such as whisker deprivation may affect neuroanatomical aspects of the brain during developmental processes. The present study designed to investigate the possible effects of whisker deprivation on the morphometric characteristics of NADPH-d positive neurons in the barrel field cortex of adolescent rats.

Materials and Methods: Pups were divided into the intact (n=4) and whisker-deprived groups (n=4). In whisker-deprived group, the total whiskers of subjects were trimmed every other day from postnatal day (PND) 0 to PND 60. NADPH-d histochemistry reaction was processed to quantitatively analyze the feature of NADPH-d containing neurons of barrel cortex.

Results: Our results showed that the number of NADPH-d positive neurons remained unchanged in whisker-deprived group compared to controls. The mean soma diameter, dendritic length and the number of 3rd order processes were significantly decreased in the whisker-deprived rats (p<0.05).

Conclusion: Our results indicate that postnatal whisker deprivation possibly alter NADPH-d/NOS neuronal features in the barrel cortex. The functional implications of these data may relate the plasticity of synaptic receptive field and developmental brain circuits.


Keywords

Barrel cortex, Nitric oxide, Plasticity, Whisker deprivation

References

. Oberlaender M, Ramirez A, Bruno RM. Sensory experience restructures thalamocortical axons during adulthood. Neuron. 2012;74(4):648-55.

Land PW, Simons DJ. Cytochrome oxidizer staining in the rat SmI barrel cortex. J Comp Neurol. 1985;238(2):225-35.

Schubert D, Kotter R, Staiger JF. Mapping functional connectivity in barrel-related columns reveals layer- and cell type-specific microcircuits. Brain Struct Funct 2007; 212(2): 107-19.

Waite PME, Tracey DJ. Trigeminal sensory system. In: Paxinos G, (Editor). The Rat Nervous System. 3rd ed. New York: Elsevier Academic Press.2004. p. 815-834.

Woolsey TA, Van der Loos H. The structural organization of layer IV in the somatosensory region (SI) of mouse cerebral cortex. The description of a cortical field composed of discrete cytoarchitectonic units. Brain Res. 1970;17(2):205-42.

Feldmeyer D, Brecht M, Helmchen F, Petersen CC, Poulet JF, Staiger JF, et al. Barrel cortex function. Prog Neurobiol. 2013;103:3-27.

Lee LJ, Chen WJ, Chuang YW, Wang YC. Neonatal whisker trimming causes long-lasting changes in structure and function of the somatosensory system. Exp Neurol. 2009;219(2):524-32.

Contestabile A. Roles of NMDA receptor activity and nitric oxide production in brain development. Brain Res Brain Res Rev. 2000;32(2-3):476-509.

Barbaresi P, Fabri M, Mensa E. Characterization of NO-producing neurons in the rat corpus callosum. Brain Behav. 2014;4(3):317-36.

Reif A, Jacob CP, Rujescu D, Herterich S, Lang S, Gutknecht L, et al. Influence of functional variant of neuronal nitric oxide synthase on impulsive behaviors in humans. Arch Gen Psychiatry. 2009;66(1):41-50.

Liuzzi FJ, Wu W, Scoville SA, Schinco FP. Development of nitric oxide synthase expression in the superficial dorsal horn of the rat spinal cord. Exp Neurol. 1993;121(2):275-8.

Hope BT, Michael GJ, Knigge KM, Vincent SR. Neuronal NADPH diaphorase is a nitric oxide synthase. Proc Natl Acad Sci U S A. 1991;88(7):2811-4.

Shlosberg D, Buskila Y, Abu-Ghanem Y, Amitai Y. Spatiotemporal alterations of cortical network activity by selective loss of NOS-expressing interneurons. Front Neural Circuits. 2012;6:3.

Jung J, Na C, Huh Y. Alterations in nitric oxide synthase in the aged CNS. Oxid Med Cell Longev. 2012;2012:718976.

Nogueira-Campos AA, Finamore DM, Imbiriba LA, Houzel JC, Franca JG. Distribution and morphology of nitrergic neurons across functional domains of the rat primary somatosensory cortex. Front Neural Circuits. 2012;6:57.

Wu MD, Kimura M, Hiromichi I, Helfert RH. A classification of NOergic neurons in the inferior colliculus of rat according to co-existence with classical amino acid transmitters. Okajimas Folia Anat Jpn. 2008;85(1):17-27.

Land PW, de Blas AL, Reddy N. Immunocytochemical localization of GABAA receptors in rat somatosensory cortex and effects of tactile deprivation. Somatosens Mot Res. 1995;12(2):127-41.

Briner A, De Roo M, Dayer A, Muller D, Kiss JZ, Vutskits L. Bilateral whisker trimming during early postnatal life impairs dendritic spine development in the mouse somatosensory barrel cortex. J Comp Neurol. 2010;518(10):1711-23.

Akbari Z, Rohani MH, Behzadi G. NADPH-d/NOS reactivity in the lumbar dorsal horn of congenitally hypothyroid pups before and after formalin pain induction. Int J Dev Neurosci. 2009;27(8):779-87.

Paxinos G, Watson C. The rat brain in stereotaxic coordinates. 6th ed. San Diego: Academic Press; 2007.

Hinova-Palova DV, Edelstein L, Landzhov B, Minkov M, Malinova L, Hristov S, et al. Topographical distribution and morphology of NADPH-diaphorase-stained neurons in the human claustrum. Front Syst Neurosci. 2014;8:96.

Jacobs B, Schall M, Prather M, Kapler E, Driscoll L, Baca S, et al. Regional dendritic and spine variation in human cerebral cortex: a quantitative golgi study. Cereb Cortex. 2001;11(6):558-71.

Xu X, Wang Z, Li Q, Xiao X, Lian Q, Xu W, et al. Endothelial nitric oxide synthase expression is progressively increased in primary cerebral microvascular endothelial cells during hyperbaric oxygen exposure. Oxid Med Cell Longev. 2009;2(1):7-13.

Hardingham N, Dachtler J, Fox K. The role of nitric oxide in pre-synaptic plasticity and homeostasis. Front Cell Neurosci. 2013;7:190.

Yan XX, Jen LS, Garey LJ. NADPH-diaphorase-positive neurons in primate cerebral cortex colocalize with GABA and calcium-binding proteins. Cereb Cortex. 1996;6(3):524-9.

Gonzalez-Soriano J, Contreras-Rodriguez J, Martinez-Sainz P, Martin-Palacios S, Marin-Garcia P, Rodriguez-Veiga E. NADPH-diaphorase distribution in the rabbit superior colliculus and co-localization with calcium-binding proteins. J Anat. 2002;200(Pt 3):297-308.

Beal MF, Kowall NW, Swartz KJ, Ferrante RJ. Homocysteic acid lesions in rat striatum spare somatostatin-neuropeptide Y (NADPH-diaphorase) neurons. Neurosci Lett. 1990;108(1-2):36-42.

Croul-Ottman CE, Brunjes PC. NADPH diaphorase staining within the developing olfactory bulbs of normal and unilaterally odor-deprived rats. Brain Res. 1988;460(2):323-8.

Ueno H, Shoshi C, Suemitsu S, Usui S, Sujiura H, Okamoto M. Somatosensory and visual deprivation each decrease the density of parvalbumin neurons and their synapse terminals in the prefrontal cortex and hippocampus of mice. Acta Med Okayama. 2013;67(3):135-43.

Freire MA, Gomes-Leal W, Carvalho WA, Guimaraes JS, Franca JG, Picanco-Diniz CW, et al. A morphometric study of the progressive changes on NADPH diaphorase activity in the developing rat's barrel field. Neurosci Res. 2004;50(1):55-66.

Micheva KD, Beaulieu C. Neonatal sensory deprivation induces selective changes in the quantitative distribution of GABA-immunoreactive neurons in the rat barrel field cortex. J Comp Neurol. 1995;361(4):574-84.

Fox K, Wong RO. A comparison of experience-dependent plasticity in the visual and somatosensory systems. Neuron. 2005;48(3):465-77.

Glazewski S. Experience-dependent changes in vibrissae evoked responses in the rodent barrel cortex. Acta Neurobiol Exp (Wars). 1998;58(4):309-20.

Wimmer VC, Broser PJ, Kuner T, Bruno RM. Experience-induced plasticity of thalamocortical axons in both juveniles and adults. J Comp Neurol. 2010;518(22):4629-48.

Chau LS, Akhtar O, Mohan V, Kondilis A, Galvez R. Rapid adult experience-dependent anatomical plasticity in layer IV of primary somatosensory cortex. Brain Res. 2014;1543:93-100.

Freire MA, Rocha EG, Oliveira JL, Guimaraes JS, Silveira LC, Elston GN, et al. Morphological variability of NADPH diaphorase neurons across areas V1, V2, and V3 of the common agouti. Brain Res. 2010;1318:52-63.

Stepanyants A, Hof PR, Chklovskii DB. Geometry and structural plasticity of synaptic connectivity. Neuron. 2002;34(2):275-88.

Sitnikova E. Neonatal sensory deprivation promotes development of absence seizures in adult rats with genetic predisposition to epilepsy. Brain Res. 2011;1377:109-18.




DOI: https://doi.org/10.22037/nbm.v3i2.8677