Frequency of Intestinal Parasitic Infections among Individuals Referred to the Medical Center Laboratories in Nahavand City, Hamadan Province, Western Iran
Background: Intestinal parasitic infections (IPIs) cause serious public health problem in the world, especially those located in tropical and subtropical areas. This study was conducted with the aim of obtaining frequency of intestinal parasites in referred people to the Nahavand city laboratories, Hamadan province, western Iran.
Materials and Methods: A comparative cross-sectional study was conducted among checkup individuals and patients referred to laboratories of Nahavand County. A total of 371 stool samples (150 from checkup individuals and 221 from patients) were selected by using systematic random sampling during summer 2014. The stool specimens were examined macroscopically, and microscopically by using direct slide smear (saline wet mount and lugol staining), formaldehyde - diethyl ether concentration, trichrome staining and modified Ziehl-Neelsen staining techniques. The results were analyzed using SPSS version 16 and Chi-square test.
Results: Ninety two patients (24.8%) were infected with single or multiple intestinal parasites. The overall prevalence of IPIs in checkup individuals and patients was 21.3% and 27.1%, respectively. The frequency of the observed intestinal parasites was: Blastocystis spp. 72 (19.4%), Entamoeba coli 7 (1/9%), Endolimax nana 7 (1/9%), Giardia lamblia 5 (1/3%), Cryptosporidium spp. 3 (0.8%), Entamoeba hartmanni 3 (0.8%), Entamoeba histolitica/E. dispar 1 (0.3%), Trichomonas hominies 1 (0.3%), Chilomastix mesnili 1 (0.3%), Iodamoeba butschlii 1 (0.3% ) and Enterobius vermicularis egg l (0.3%).
Conclusion: The proportion of observed protozoan parasites 91 (24.5%) is higher than helminthes infection 1 (0.3%). The worm infections in Nahavand city was dramatically decreased over the past decades, induced increases in public health at the community level. Blastocystis spp. was the predominant intestinal parasite in people referred to the Nahavand city laboratories. Proportion of pathogenic IPIs among patients 4.07% (9 of 221) was higher in compare to the checkup individuals in which only one out of 150 (0.66%) Giardia lamblia was observed.
Ravdin Ji. Amebiasis. Clinical Infectious Diseases. 1995;1453-64.
Asenso-Okyere K, Chiang C, Andam K. Interactions between health and farm-labor productivity: Intl Food Policy Res Inst. 2011.
Eick R. An analysis of the presentation and management of intestinal parasites by short-term medical mission volunteers in paraguay. Parasites in Paraguay: Boonshoft School of Medicine
Wright State University. 2013.
Dagci H, Kurt Ö, Demirel M, Östan I, Azizi Nr, Mandiracioglu A, et al. The prevalence of intestinal parasites in the province of Izmir, Turkey. Parasitology Research. 2008;103(4):839-45.
Mengistu A, Gebre-Selassie S, Kassa T. Prevalence of intestinal parasitic infections among urban dwellers in southwest Ethiopia. Ethiopian Journal of Health Development. 2007;21(1):12-7.
Badparva E, Kheirandish F, Ebrahimzade F. Prevalence of intestinal parasites in Lorestan Province, West of Iran. Asian Pacific Journal of Tropical Disease. 2014;4:728-32.
Haque R, Mondal D, Kirkpatrick BD, Akther S, Farr BM, Sack RB, et al. Epidemiologic and clinical characteristics of acute diarrhea with emphasis on Entamoeba histolytica infections in preschool children in an urban slum of Dhaka, Bangladesh. The American Journal of Tropical Medicine and Hygiene. 2003;69(4):398-405.
Faustini A, Marinacci C, Fabrizi E, Marangi M, Recchia O, Pica R, et al. The impact of the Catholic Jubilee in 2000 on infectious diseases. A case-control study of giardiasis, Rome, Italy 2000–2001. Epidemiology and Infection. 2006;134(03):649-58.
Stanley Jr Sl. Amoebiasis. The Lancet. 2003; 361(9362):1025-34.
Medema G, Teunis P, Blokker M, Deere D, Davison A, Charles P, et al. WHO guidelines for drinking water quality: Cryptosporidium. WHO, New York. 2006;138.
De Silva Nr, Brooker S, Hotez Pj, Montresor A, Engels D, Savioli L. Soil-transmitted helminth infections: updating the global picture. Trends in parasitology. 2003;19(12):547-51.
Haghighi A, Khorashad A, Mojarad E, Kazemi B, Nejad M, Rasti S. Frequency of enteric protozoan parasites among patients with gastrointestinal complaints in medical centers of Zahedan, Iran. Transactions Of The Royal Society Of Tropical Medicine And Hygiene. 2009;103(5):452-4.
Kheirandish F, Tarahi Mj, Ezatpour B. Prevalence of intestinal parasites among food handlers in western iran. Revista Do Instituto De Medicina Tropical De São Paulo. 2014;56(2):111-4.
Rokni M. The present status of human helminthic diseases in Iran. Annals of Tropical Medicine And Parasitology. 2008;102(4):283-95.
Sayyari A, Imanzadeh F, Bagheri Yazdi S, Karami H, Yaghoobi M. Prevalence of intestinal parasitic infections in the islamic republic of Iran. East Mediterr Health J. 2005;11(3):377-83.
Niyyati M, Rezaeian M, Zahabion F, Hajarzadeh R, Kia E. A survey on intestinal parasitic infections in patients referred to a hospital in tehran. Pak J Med Sci. 2009;25(1):87-90.
Fallah M, Haghighi A. Cryptosporidiosis in children with diarrhea submitted to health centers in the west of Iran (Hamedan). Medical Journal of the Islamic Republic of Iran (Mjiri). 1996;9(4):315-7.
Jafari R, Fallah M, Yousofi Darani H, Yousefi HA, Mohaghegh MA, Latifi M, et al. Prevalence of intestinal parasitic infections among rural inhabitants of Hamadan city, Iran, 2012. Avicenna J Clin Microbiol Infect. 2014;1:E21445.
Garcia L. Laboratory identification of the microsporidia. Journal of Clinical Microbiology. 2002;40(6):1892-901.
Balcioglu IC, Kurt Ö, Limoncu ME, Dinç G, Gümüş M, Kilimcioglu AA, et al. Rural life, lower socioeconomic status and parasitic infections. Parasitology International. 2007;56(2):129-33.
Okyay P, Ertug S, Gultekin B, Onen O, Beser E. Intestinal parasites prevalence and related factors in school children, a western city sample-turkey. BMC Public Health. 2004;4(1):64.
Moosavi A, Haghighi A, Mojarad EN, Zayeri F, Alebouyeh M, Khazan H, Et Al. Genetic variability of blastocystis sp. Isolated from symptomatic and asymptomatic individuals in Iran. Parasitology Research. 2012;111(6):2311-5.
Mowlavi G, Mirahmadi H, Rezaeian M, Kia E, Rokni M, Golestan B, et al. Prevalence of intestinal parasites in tribal parts of khuzestan province during 2005-07. Govaresh. 2008;12(4):219-28.
Kuzehkanani Ab, Rezaei S, Babaei Z, Niyyati M, Hashemi S, Rezaeian M. Enteric protozoan parasites in rural areas of bandar-abbas, southern iran: comparison of past and present situation. Iranian Journal of Public Health. 2011;40(1):80.
Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clinical Microbiology Reviews. 2008;21(4):639-65.
Kia E, Hosseini M, Nilforoushan M, Meamar A, Rezaeian M. Study of intestinal protozoan parasites in rural inhabitants of mazandaran province, northern iran. Iranian Journal Of Parasitology. 2008;3(1):21-5.
Hooshyar H, Bagherian T, Baghbani F. Prevalence of intestinal parasitic infections among patients referred to Kashan reference laboratory in 2007-2011. Jundishapur Journal of Health Sciences. 2013;5(1):18-22.