Mycoplasma Infection in Pyospermic Infertile and Healthy Fertile Men
Background: Infections are one of the correctable causes of infertility with low cost and cost effective treatment. The 50% of infertile cases is related to men in some way, and 30% of them are absolutely related to them. Mycoplasmas are the smallest microorganisms with capability of DNA replication. Present study is planned to compare the mycoplasma infection in infertile men and men with established fertility.
Materials and Methods: 45 Semen samples were collected from case and control persons who reffered to Royan Infertility and Fertility Institute between 2004 and 2005 and stored in -20°C until time of test. DNA was extracted from semen using phenol chloroform. PCR reaction was done by mycoplasma specific primers.
Results: Mycoplasma genitalium gene was amplified in 6 (40%) cases from 15 infertile semen samples and 11 (36.6%) from 30 control semen samples.
Conclusion: Probability of genital infection, at least, in these studies group, is very lower than other communities' reports.
Blandford JM, Gift TL. Productivity losses attributable to untreated chlamydial infection and associated pelvic inflammatory disease in reproductive-aged women. Sex Transm Dis. 2006;33(10 Suppl):117-21.
Paavonen J, Eggert- Kruse W. Chlamydia trachomatis: impact on human reproduction. Hum reprod Update. 1999; 5(5): 433-47.
Yoshida K, Kobayashi N, Negishi T. Chlamydia trachomatis infection in the semen of asymptomatic infertile men: detection of the antigen by in situ hybridization. Urol Int. 1994;53(4):217-21.
Ochsendorf FR, Ozdemir K, Rabenau H, Fenner T, Oremek R, Milbradt R, et al. Chlamydia trachomatis and male infertility: chlamydia-IgA antibodies in seminal plasma are C. trachomatis specific and associated with an inflammatory response. J Eur Acad Dermatol Venereol. 1999;12(2):143-52.
Gonzalez-jimenez MA, Villanueva-Diaz CA. Epididymal stereocilia in semen of infertile men: evidence of chronic epididymitis? Andrologia. 2006;38(1):26-30.
Blattner RJ. Mycoplasma infection. J Pediatr. 1968;72(4):554-6.
Köchl S, Niederstätter H, Parson W. DNA extraction and quantitation of forensic samples using the phenol-chloroform method and real-time PCR. Methods Mol Biol. 2005;297:13-30.
Salari MH, Karimi A. Prevalence of ureaplasma urealyticum and mycoplasma genitalium in men with non-gonococcal urethritis. East Mediterr Health. 2003;9(3):291-5.
Zhao J, Xu C, Wang N. Epidemiological study on the infections of Mycoplasma and Chlamydia in immoral persons and healthy controls in seven areas of China Zhonghua Liu Xing Bing Hue Za Zhi. 1994;15(5): 263-6.
Maeda S, Deguchi T, Ishiko H, Matsumoto T, Naito S, Kumon H, et al. Detection of mycoplasma genitalium, mycoplasma hominis, ureaplasma parvum (biovar 1) and ureaplasma urealyticum (biovar 2) in patients with non-gonococcal urethritis using polymerase chain reaction-microtiter plate hybridization. Int J Urol. 2004;11(9):750-4.
Leung A, Fastick K Haddon LE, Horn CK, Ahuja D, Horner PJ. Mycoplasma genitalium is associated with symptomatic urethritis. Int J STD. 2006;17(5):285-8.
Mellenius H, Boman J, Lundgvist EN, Jensen JS. Mycoplasma genitalium should be suspected in unspecific urethritis and cervicitis. A study from Vasterbotten confirms the high prevalence of the bacteria. Lakartidningen. 2005;102(47):3538, 3540-1.
Anagrius C, Lore B, Jensen JS. Mycoplasma genitalium: prevalence, clinical significance, and transmission. Sex Transm Infect. 2005;81(6):458-62.
Daxboeck F, Zitta S, Stadler M, Iro E, Krause R. Mycoplasma hominis and ureaplasma urealyticum in patients with sterile pyuria. J Infect. 2005;51(1):54-8.
Jarvi K, Noss MB. Pyospermia and male infertility. Can J Urol. 1994;1(2):25-30.
Sigman M, Lopes L. The correlation between round cells and white blood cells in the semen. J Urol. 1993;149(5 Pt 2):1338-40.
Arata de Bellabarba G, Tortolero I, Villarroel V, Molina CZ, Bellabarba C, et al. (2000). Nonsperm cells in human semen and their relationship with semen parameters. Arch Androl. 2000;45(3):131-6.
Campbell M, Patric C. Campbell`s Urology, 8th edition, Philadelphia Saunders. 2002;1047–8.
Tsunoe H, Tanaka M, Nakayama H, Sano M, Nakamura G, Shin T, et al. High prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in female commercial sex workers in Japan. Int J STD AIDS. 2000;12:790-4.
Atkinson TP, Balish MF, Waites KB. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol Rev. 2008;32(6):956-73.
Waites KB, Balish MF, Atkinson TP. New insights into the pathogenesis and detection of Mycoplasma pneumonia Infections. Future Microbiol. 2008;3(6):635–48.
Miller-Catchpole R, Shattuck M, Kandalaft P, Variakojis D, Anastasi J, Abrahams C. The incidence and distribution of Mycoplasma fermentans (incognitus strain) in the Chicago AIDS autopsy series: an immunohistochemical study. Chicago Associated Pathologists. Mod Pathol. 1991;4(4):481-6.
Watson ME Jr, Storch GA. Recurrent Mycoplasma pneumoniae infection in a human immunodeficiency virus-positive child. Pediatr Infect Dis J. 2008;27(11):1037-8.
Shankar EM, Kumarasamy N, Vignesh R, Balakrishnan P, Solomon SS, Murugavel KG, et al. Epidemiological studies on pulmonary pathogens in HIV-positive and -negative subjects with or without community-acquired pneumonia with special emphasis on Mycoplasma pneumoniae. Jpn J Infect Dis. 2007;60(6):337-41.