Genotyping of Virulence Factors of Uropathogenic Escherichia coli by PCR
Novelty in Biomedicine,
Vol. 3 No. 4 (2015),
28 October 2015
,
Page 177-181
https://doi.org/10.22037/nbm.v3i4.8036
Abstract
Background: Escherichia coli is the most causative agent of urinary tract infections (UTIs). Apart from all human infectious diseases, UTI have a high prevalence and in most cases, Escherichia coli is a dominance bacterium which can cause pyelonephritis and cystitis. The aim of the study was to determine the occurrence of some virulence genes expressing fimbriae, production of hemolysin and aerobactin among a hundred Escherichia coli isolates obtained from in-and outpatients of Karaj Shahid Rajaii hospital, showing clinical and laboratory signs of UTI.
Materials and Methods: In this investigation we isolated Escherichia coli strains from urine samples of patients with UTI during the period of July to December 2012 and studied them for the presence of the virulence genes by PCR.
Results and Conclusion: The most abundant virulence factor in this study was fimH. The prevalence of the virulence factors for fimbriae type 1 (fimH gene), pyelonephritis associated pili (pap gene), S-family adhesions (sfa gene), hemolysin (hly gene) and aerobactin (aer gene), was 73%, 46%, 32%, 47%, 57%, respectively.
- Escherichia coli
- Urinary tract infection
- Virulence factors
- PCR
How to Cite
References
Santo E, Macedo C, Marin JM. Virulence factors of uropathogenic Escherichia coli from a university hospital in Ribeirao Preto, Sao Paulo, Brazil. Rev Inst Med Trop S Paulo. 2006;48(4):185-8.
Bahalo S, Tajbakhsh E, Tajbakhsh S, Momeni M, Tajbakhsh F. Detection of some virulence factors of Escherichia coli isolated from urinary tract infection isolated of children in Shahrekord Iran by Multiplex PCR. Middle-East J Scien Res. 2013;14(1):29-32.
Ribeiro TIBA M, Yano T, Da Silva LEITE D. Genotypic characterization of virulence factors in Escherichia coli strains from patients with cyctitis. Rev Inst Med Trop S Paulo. 2008;50(5):255-60.
Arisoy M, Aysev D, Ekim M, Ozel D, Kose K, Ozsoy ED, et al. Detection of virulence factors of Escherichia coli from children by multiplex PCR. Int J Clin Prac. 2005;60(2):170-3.
Le Bouguenec C, Archambaud M, Labigne A. Rpid and specific detection of the pap, afa, and sfa adhesin encoding operons in uropathogenic Escherichia coli strains by PCR. J Clin Mic. 1992:1189-93.
Yamamoto S, Terai A, Yuri K, Kurazono H, Takeda Y, Yoshida O. Detection of urovirulence factors in Escherichia coli by multiplex PCR. FEMS Immuno Med Mic. 1995;12:85-90.
Karimian A, Momtaz H, Madani M. Detection of uropathogenic Escherichia coli virulence factors in patients with urinary tract infections in Iran. African J Mic Res. 2012;6(39):6811-6.
Tarchouna M, Ferjani A, Selma WB, Boukadida J. Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection. Int J Infect Dis. 2013;17:450-3.
Oliveira FA, Paludo KS, Arend LNVS, Farah SMSS, Pedrosa FO, Souza EM, et al. Virulence characteristics and antimicrobial susceptibility of uropathogenic Escherichia coli strains. Genetics and Molecular Res. 2011;10(4):4114-25.
Mohajeri P, Khademi H, Ebrahimi R, Farahani A, Rezaei M. Frequency distribution of virulence factors in uropathogenic Escherichia coli isolated from Kermanshah in 2011-2012. Int J App Basic Med Res. 2014;4:111-6.
Soto SM, Zuniga P, Ulleryd P, Vila J. Acquisition of a pathogenicity island in an Escherichia coli clinical isolates causing febrile urinary tract infection. Eur J Clin Microbiol Infect Dis. 2011;30:1543-50.
Abe CM, Salvador FA, Falsetti IN, Vieira MA, et al. Uropathogenic Escherichia coli (UPEC) strains may carry virulence properties of diarrhoeagenic E. Coli. FEMS Immunoi Med Microbiol. 2008;52:397-406.
Blanco M, Blanco JE, Rodriguez E, Abalia I, et al. Detection of virulence genes in uropathogenic Escherichia coli by PCR comparison with results obtained using phenotypic methods. J Clin Micro. 1997;31:37-43.
Antao EM, Wieler LH and Ewers C. Adhesive threads of extraintestinal pathogenic Escherichia coli. Gut Pathog. 2009;1:22.
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