Cytotoxic Activity of Rosa Damascene Mill, Allium sativum, Allium Hirtifolium Boiss, and Prosopis Farcta Extracts on Human Cervical Carcinoma Cell Line
Background: Cervical cancer is one of the major reasons of cancer-related mortality. Human papillomavirus is the most common sexually transmitted viral infection, which can lead to cervical cancer. There is no powerful chemotherapeutic agent for HPV infection and cervical cancer. Some plants have the proper potential to be used for treatment of cervical cancer caused by HPV type 18.
Materials and Methods: In this study, cytotoxic effect of extract of four indigenous Iranian plants including Rosa damascene mill, Allium sativum, Allium hirtifolium boiss and Prosopis farcta were investigated on the HeLa cell line. HeLa cells were incubated with different concentrations of extracts and then the cell viability was measured by MTT assay.
Results: The viable cell numbers were decreased by increase of the extracts concentration. The Allium sativum showed the higher cytotoxicity in all concentrations than the other ones. Afterwards, Allium hirtifolium Boiss, Rosa damascene mill, and Prosopis farcta showed maximum efficiency to decrease cell viability, respectively.
Conclusion: The above four mentioned plants might be used for death of HeLa cell harboring HPV type 18. Therefore, they could be employed as a chemotherapeutic agent in the cervical cancer treatment in future.
Young LS, Dawson CW. Epstein-Barr virus and nasopharyngeal carcinoma. Chinese journal of cancer. 2014;33(12):581.
Sawyer BG, Esquivel D, Smith C, Carter TY. The Detection of HPV-Infected Cells Using Real-time PCR. Clinical Laboratory Science. 2015;28(3).
Moscicki A-B. Impact of HPV infection in adolescent populations. Journal of Adolescent Health. 2005;37(6):3-9.
Maggino T, Casadei D, Panontin E, Fadda E, Zampieri M, Dona M, et al. Impact of an HPV diagnosis on the quality of life in young women. Gynecologic oncology. 2007;107(1):175-9.
Insinga RP, Glass AG, Rush BB. The health care costs of cervical human papillomavirus–related disease. American journal of obstetrics and gynecology. 2004;191(1):114-20.
Forman D, de Martel C, Lacey CJ, Soerjomataram I, Lortet-Tieulent J, Bruni L, et al. Global burden of human papillomavirus and related diseases. Vaccine. 2012;30:12-23.
Vinodhini K, Shanmughapriya S, Das BC, Natarajaseenivasan K. Prevalence and risk factors of HPV infection among women from various provinces of the world. Archives of gynecology and obstetrics. 2012;285(3):771-7.
Chao A, Lin C-T, Lai C-H. Updates in systemic treatment for metastatic cervical cancer. Current treatment options in oncology. 2014;15(1):1-13.
Eskander RN, Tewari KS. Chemotherapy in the treatment of metastatic, persistent, and recurrent cervical cancer. Current Opinion in Obstetrics and Gynecology. 2014;26(4):314-21.
Taraphdar AK, Roy M, Bhattacharya R. Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Current science. 2001:1387-96.
Tiwary BK, Bihani S, Kumar A, Chakraborty R, Ghosh R. The in vitro cytotoxic activity of ethno-pharmacological important plants of Darjeeling district of West Bengal against different human cancer cell lines. BMC complementary and alternative medicine. 2015;15(1):22.
Jahdi F, Khademi K, Khoei EM, Haghani H, Yazdanpanahi Z. Evaluation of the individual and medical factors associated with genital human papillomavirus in Iranian women. Scimetr. 2014;2(4).
Kawana K, Yasugi T, Taketani Y. Human papillomavirus vaccines: current issues & future. Indian Journal of Medical Research. 2009;130(3):341.
Oommen S, Anto RJ, Srinivas G, Karunagaran D. Allicin (from garlic) induces caspase-mediated apoptosis in cancer cells. European Journal of Pharmacology. 2004;485(1-3):97-103.
Islam MS, Kusumoto Y, Al-Mamun MA. Cytotoxicity and Cancer (HeLa) Cell Killing Efficacy of Aqueous Garlic (Allium sativum) Extract. Journal of Scientific Research. 2011; 3(2).
Hosseini FS, Falahati-Pour SK, Hajizadeh MR, Khoshdel A, Mirzaei MR, Ahmadirad H, et al. Persian shallot, Allium hirtifolium Boiss, induced apoptosis in human hepatocellular carcinoma cells. Cytotechnology. 2017;69(4):551-63.
Ghodrati Azadi H, Ghaffari SM, Riazi GH, Ahmadian S, Vahedi F. Antiproliferative activity of chloroformic extract of Persian Shallot, Allium hirtifolium, on tumor cell lines. Cytotechnology. 2008;56(3):179-85.
Omidi A, Ansari nik H, Ghazaghi M. Prosopis farcta beans increase HDL cholesterol and decrease LDL cholesterol in ostriches (Struthio camelus). Tropical animal health and production. 2013;45(2):431-4.
Zamiri-Akhlaghi A, Rakhshandeh H, Tayarani-Najaran Z, Mousavi SH. Study of cytotoxic properties of Rosa damascena extract in human cervix carcinoma cell line. Avicenna Journal of Phytomedicine. 2011;1(2):74-7.
Haghi G, Hatami A. Simultaneous quantification of flavonoids and phenolic acids in plant materials by a newly developed isocratic high-performance liquid chromatography approach. Journal of agricultural and food chemistry. 2010;58(20):10812-6.
Zu Y, Yu H, Liang L, Fu Y, Efferth T, Liu X, et al. Activities of ten essential oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 cancer cells. Molecules. 2010;15(5):3200-10.