An Evaluation of the Plasma Levels of Frequently Used Pesticides in Dairy Cattle and Its Possible Correlation with the Occurrence of Follicular Cystic Ovarian Disease: A Case-Control Study
Background: Cystic ovarian disease (COD) is one of the common reproductive disorders which affecting the fertility of dairy cattle induces heavy financial burdens on herds owners. Various insecticides, fungicides and herbicides, collectively known as pesticides are frequently used in the agricultural systems of different countries. Given the fact that pesticides are known to have endocrine disrupting properties, exposure to these compounds may play a role in the development of COD.
Materials and Methods: The plasma concentrations of a complete profile of common pesticides including organophosphorus, organochlorine, and carbamate and pyrethroid compounds in the plasma of cattle with COD compared to healthy controls was examined. Moreover, plasma concentrations of inflammatory cytokines as well as oxidative stress parameters were investigated.
Results: No significant amounts of any of the pesticides investigated were detectable in the plasma of neither the healthy nor cystic cows. The plasma indices of total antioxidant capacity (TAC), thiol, lipid peroxidation (LPO), and reactive oxygen species (ROS) did not show any significant differences between the affected and the control groups. Tumor necrosis factors alpha (TNF-α), progesterone, lymphocyte, neutrophil, fibrinogen and MCHC had significantly higher amounts in the plasma of COD cows.
Conclusion: Findings of the present study do not support the notion that exposure to the studied pesticides is a contributing factor in the development of follicular cysts in dairy cattle. In addition, TNF-α might be affected as a factor in the pathogenesis of COD by an independent pathway of pesticides effect.
Kesler D, Garverick H. Ovarian cysts in dairy cattle: a review. Journal of animal science. 1982;55(5):1147-59.
Vanholder T, Opsomer G, De Kruif A. Aetiology and pathogenesis of cystic ovarian follicles in dairy cattle: a review. Reproduction Nutrition Development. 2006;46(2):105-19.
Farin PW, Estill CT. Infertility due to abnormalities of the ovaries in cattle. Veterinary Clinics of North America: Food Animal Practice. 1993;9(2):291-308.
Azziz R, Marin C, Hoq L, Badamgarav E, Song P. Health care-related economic burden of the polycystic ovary syndrome during the reproductive life span. The Journal of Clinical Endocrinology and Metabolism. 2005;90(8):4650-8.
Marelli BE, Diaz PU, Salvetti NR, Rey F, Ortega HH. mRNA expression pattern of gonadotropin receptors in bovine follicular cysts. Reproductive biology. 2014;14(4):276-81.
Silvia W, Hatler T, Nugent A, Da Fonseca LL. Ovarian follicular cysts in dairy cows: an abnormality in folliculogenesis. Domestic animal endocrinology. 2002;23(1):167-77.
Yoshioka K, Iwamura S, Kamomae H. Ultrasonic observations on the turnover of ovarian follicular cysts and associated changes of plasma LH, FSH, progesterone and oestradiol-17β in cows. Research in veterinary science. 1996;61(3):240-4.
Matiller V, Stangaferro M, Díaz P, Ortega H, Rey F, Huber E, et al. Altered Expression of Transforming Growth Factor‐Beta Isoforms in Bovine Cystic Ovarian Disease. Reproduction in Domestic Animals. 2014;49(5):813-23.
Baravalle M, Stassi A, Velázquez M, Belotti E, Rodriguez F, Ortega H, et al. Altered expression of pro-inflammatory cytokines in ovarian follicles of cows with cystic ovarian disease. Journal of comparative pathology. 2015;153(2):116-30.
Díaz PU, Stangaferro ML, Gareis NC, Silvia WJ, Matiller V, Salvetti NR, et al. Characterization of persistent follicles induced by prolonged treatment with progesterone in dairy cows: An experimental model for the study of ovarian follicular cysts. Theriogenology. 2015;84(7):1149-60.
Rizzo A, Minoia G, Trisolini C, Mutinati M, Spedicato M, Jirillo F, et al. Reactive Oxygen Species (ROS): involvement in bovine follicular cysts etiopathogenesis. Immunopharmacology and immunotoxicology. 2009;31(4):631-5.
Maqbool F, Mostafalou S, Bahadar H, Abdollahi M. Review of endocrine disorders associated with environmental toxicants and possible involved mechanisms. Life sciences. 2016;145:265-73.
Ventura C, Nieto MRR, Bourguignon N, Lux-Lantos V, Rodriguez H, Cao G, et al. Pesticide chlorpyrifos acts as an endocrine disruptor in adult rats causing changes in mammary gland and hormonal balance. The Journal of steroid biochemistry and molecular biology. 2016;156:1-9.
Asghari MH, Moloudizargari M, Bahadar H, Abdollahi M. A review of the protective effect of melatonin in pesticide-induced toxicity. Expert Opinion on Drug Metabolism & Toxicology. 2016:1-10.
Hodjat M, Rezvanfar MA, Abdollahi M. A systematic review on the role of environmental toxicants in stem cells aging. Food and Chemical Toxicology. 2015;86:298-308.
Mokarizadeh A, Faryabi MR, Rezvanfar MA, Abdollahi M. A comprehensive review of pesticides and the immune dysregulation: mechanisms, evidence and consequences. Toxicology mechanisms and methods. 2015;25(4):258-78.
Mostafalou S, Abdollahi M. Pesticides and human chronic diseases: evidences, mechanisms, and perspectives. Toxicology and applied pharmacology. 2013;268(2):157-77.
Karami-Mohajeri S, Abdollahi M. Toxic influence of organophosphate, carbamate, and organochlorine pesticides on cellular metabolism of lipids, proteins, and carbohydrates: a systematic review. Human & experimental toxicology. 2011;30(9):1119-40.
Rahimifard M, Navaei-Nigjeh M, Mahroui N, Mirzaei S, Siahpoosh Z, Nili-Ahmadabadi A, et al. Improvement in the function of isolated rat pancreatic islets through reduction of oxidative stress using traditional Iranian medicine. Cell J (Yakhteh). 2014;16(2):1-9.
Astaneie F, Afshari M, Mojtahedi A, Mostafalou S, Zamani MJ, Larijani B, et al. Total antioxidant capacity and levels of epidermal growth factor and nitric oxide in blood and saliva of insulin-dependent diabetic patients. Archives of Medical Research. 2005;36(4):376-81.
Benzie IF, Strain J. Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology. 1999;299:15-27.
Hu M, Dillard C. Plasma SH and GSH measurement. Methods Enzymol. 1994;233(385):87.
Lehotay SJ. Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study. J AOAC Int. 2007;90(2):485-520.
Noakes DE. Arthur's veterinary reproduction and obstetrics. Elsevier Health Sciences. 2009:431-43.
Youngquist R. Cystic follicular degeneration in the cow. Current therapy in theriogenology. 1986;2:243-6.
Balali-Mood M, Abdollahi M. Basic and clinical toxicology of organophosphorus compounds. Springer. 2014:1-10.
Talukder S, Ingenhoff L, Kerrisk K, Celi P. Plasma oxidative stress biomarkers and progesterone profiles in a dairy cow diagnosed with an ovarian follicular cyst. Veterinary Quarterly. 2014;34(2):113-7.
Vabre P, Gatimel N, Moreau J, Gayrard V, Picard-Hagen N, Parinaud J, et al. Environmental pollutants, a possible etiology for premature ovarian insufficiency: a narrative review of animal and human data. Environ Health. 2017;16(1):37.
Patel S. Disruption of aromatase homeostasis as the cause of a multiplicity of ailments: A comprehensive review. The Journal of Steroid Biochemistry and Molecular Biology. 2017; 168:19-25.
Guo Z, Qiu H, Wang L, Wang L, Wang C, Chen M, et al. Association of serum organochlorine pesticides concentrations with reproductive hormone levels and polycystic ovary syndrome in a Chinese population. Chemosphere. 2017;171:595-600.
Yang Q, Zhao Y, Qiu X, Zhang C, Li R, Qiao J. Association of serum levels of typical organic pollutants with polycystic ovary syndrome (PCOS): a case–control study. Human Reproduction. 2015;30(8):1964-73.
Rezvanfar MA, Saadat S, Shojaei Saadi HA, Mansoori P, Saeedi S, Gooshe M, et al. Cellular and molecular mechanisms of pentoxifylline's beneficial effects in experimental polycystic ovary. Theriogenology. 2015;83(6):968-77.
Rezvanfar MA, Ahmadi A, Saadi HA, Baeeri M, Abdollahi M. Mechanistic links between oxidative/nitrosative stress and tumor necrosis factor alpha in letrozole-induced murine polycystic ovary: biochemical and pathological evidences for beneficial effect of pioglitazone. Hum Exp Toxicol. 2012;31(9):887-97.
Rezvanfar MA, Rezvanfar MA, Ahmadi A, Shojaei-Saadi HA, Baeeri M, Abdollahi M. Molecular mechanisms of a novel selenium-based complementary medicine which confers protection against hyperandrogenism-induced polycystic ovary. Theriogenology. 2012;78(3):620-31.
Rezvanfar MA, Shojaei Saadi HA, Gooshe M, Abdolghaffari AH, Baeeri M, Abdollahi M. Ovarian Aging-Like Phenotype in the Hyperandrogenism-Induced Murine Model of Polycystic Ovary. Oxidative Medicine and Cellular Longevity. 2014:1-10.